Development of a novel in vitro onychomycosis model for the evaluation of topical antifungal activity.


:A novel in vitro onychomycosis model was developed to easily predict the topical activity potential of novel antifungal drugs. The model encompasses drug activity and diffusion through bovine hoof slices in a single experimental set-up. Results correspond well with the antifungal susceptibility assay and Franz cell diffusion test.


J Microbiol Methods


Sleven R,Lanckacker E,Boulet G,Delputte P,Maes L,Cos P




Has Abstract


2015-05-01 00:00:00












  • A tetracycline-inducible expression vector for Streptococcus agalactiae allowing controllable gene expression.

    abstract::Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Lartigue MF,Bouloc P

    更新日期:2014-01-01 00:00:00

  • A simple method for high molecular-weight genomic DNA extraction suitable for long-read sequencing from spores of an obligate biotroph oomycete.

    abstract::Long-read sequencing technologies are having a major impact on our approaches to studying non-model organisms and microbial communities. By significantly reducing the cost and facilitating the genome assembly pipelines, any laboratory can now develop its own genomics program regardless of the complexity of the genome ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Penouilh-Suzette C,Fourré S,Besnard G,Godiard L,Pecrix Y

    更新日期:2020-09-12 00:00:00

  • A single-pair method to screen Rickettsia-infected and uninfected whitefly Bemisia tabaci populations.

    abstract::Bacterial endosymbionts such as Rickettsia and Wolbachia play prominent roles in the development and behaviour of their insect hosts, such as whiteflies, aphids, psyllids and mealybugs. Accumulating studies have emphasized the importance of establishing experimental insect populations that are either lacking or bearin...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Liu Y,Fan ZY,An X,Shi PQ,Ahmed MZ,Qiu BL

    更新日期:2020-01-01 00:00:00

  • Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux.

    abstract::A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Tienaho J,Sarjala T,Franzén R,Karp M

    更新日期:2015-11-01 00:00:00

  • Carbohydrates and glycoproteins of Bacillus anthracis and related bacilli: targets for biodetection.

    abstract::The spore is the form released in a bioterrorism attack. There is a real need for definition of new targets for Bacillus anthracis that might be incorporated into emerging biodetection technologies. Particularly of interest are macromolecules found in B. anthracis that are (1) spore-specific, (2) readily accessible on...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Fox A,Stewart GC,Waller LN,Fox KF,Harley WM,Price RL

    更新日期:2003-08-01 00:00:00

  • Rapid antibiotic susceptibility testing by resazurin using thin film platinum as a bio-electrode.

    abstract::Traditional antibiotic susceptibility testing methods take several days to confirm and start disease treatment. The lack of new antibiotics or drugs warrants a need for optimization of current diagnostic tools for immediate antibiotic susceptibility testing. We present a rapid screening method to evaluate the response...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Mishra P,Singh D,Mishra KP,Kaur G,Dhull N,Tomar M,Gupta V,Kumar B,Ganju L

    更新日期:2019-07-01 00:00:00

  • Hybridization of genomic DNA to microarrays: a challenge for the analysis of environmental samples.

    abstract::The use of DNA microarrays for detection and identification of bacteria and genes of interest from various environments (e.g. soil, sediment, water column...) is a major challenge for microbiologists working on functional diversity. So far, most of the genomic methods that have been described rely on the use of taxono...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Avarre JC,de Lajudie P,Béna G

    更新日期:2007-05-01 00:00:00

  • Automated counting of bacterial colonies on agar plates based on images captured at near-infrared light.

    abstract::Counting colonies is usually used in microbiological analysis to assess if samples meet microbiological criteria. Although manual counting remains gold standard, the process is subjective, tedious, and time-consuming. Some developed automatic counting methods could save labors and time, but their results are easily af...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Zhu G,Yan B,Xing M,Tian C

    更新日期:2018-10-01 00:00:00

  • A novel selective medium for the isolation and distribution of Rothia dentocariosa in oral cavities.

    abstract::Rothia dentocariosa is an indigenous microbial flora of dental plaque. To examine the bacterium population in oral cavities, a novel selective medium, designated RDSM, was developed for the isolation of R. dentocariosa. RDSM was prepared by adding 0.5 μg/ml of lincomycin, 10 μg/ml of colistin, and 40% sucrose to Heart...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Uchibori S,Tsudukibashi O,Goto H,Kobayashi T,Aida M

    更新日期:2012-10-01 00:00:00

  • An improved high throughput sequencing method for studying oomycete communities.

    abstract::Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sapkota R,Nicolaisen M

    更新日期:2015-03-01 00:00:00

  • A multi-amplicon 16S rRNA sequencing and analysis method for improved taxonomic profiling of bacterial communities.

    abstract::Metagenomic sequencing of bacterial samples has become the gold standard for profiling microbial populations, but 16S rRNA profiling remains widely used due to advantages in sample throughput, cost, and sensitivity even though the approach is hampered by primer bias and lack of specificity. We hypothesized that a hybr...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Schriefer AE,Cliften PF,Hibberd MC,Sawyer C,Brown-Kennerly V,Burcea L,Klotz E,Crosby SD,Gordon JI,Head RD

    更新日期:2018-11-01 00:00:00

  • Method for evaluating mold growth on ceiling tile.

    abstract::A method to extract mold spores from porous ceiling tiles was developed using a masticator blender. Ceiling tiles were inoculated and analyzed using four species of mold. Statistical analysis comparing results obtained by masticator extraction and the swab method was performed. The masticator method was demonstrated a...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Betancourt DA,Dean TR,Menetrez MY

    更新日期:2005-06-01 00:00:00

  • Quantitative reverse transcription polymerase chain reaction analysis of Porphyromonas gingivalis gene expression in vivo.

    abstract::An etiological relationship between periodontitis, a significant oral health problem, and the anaerobe Porphyromonas gingivalis may be related to the expression of a variety of putative virulence factors. The objective of the experiments described here was to develop a quantitative reverse transcription polymerase cha...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Shelburne CE,Gleason RM,Germaine GR,Wolff LF,Mullally BH,Coulter WA,Lopatin DE

    更新日期:2002-04-01 00:00:00

  • Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro.

    abstract::Volatile compounds (VCs) are produced by all microorganisms as part of their normal metabolism. The aim of this study was to determine whether bacterial VC profiles could be used to discriminate between selected bacterial species and strains in vitro. Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) was used to quan...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Thorn RM,Reynolds DM,Greenman J

    更新日期:2011-02-01 00:00:00

  • Comparison study of membrane filtration direct count and an automated coliform and Escherichia coli detection system for on-site water quality testing.

    abstract::This study compared an automated Escherichia coli and coliform detection system with the membrane filtration direct count technique for water testing. The automated instrument performed equal to or better than the membrane filtration test in analyzing E. coli-spiked samples and blind samples with interference from Pro...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Habash M,Johns R

    更新日期:2009-10-01 00:00:00

  • Development and characterization of a 3D oral mucosa model as a tool for host-pathogen interactions.

    abstract::The aim of this study was to (i) design, develop and validate a practical and physiologically relevant reconstituted in vitro oral mucosa tissue model and (ii) to assess its applicability in in vitro host-pathogen interactions with C. albicans and S. aureus. Co-culture organotypic constructions were created by incorpo...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: de Carvalho Dias K,de Sousa DL,Barbugli PA,Cerri PS,Salih VM,Vergani CE

    更新日期:2018-09-01 00:00:00

  • Immobilization of microalgae cells in alginate facilitates isolation of DNA and RNA.

    abstract::Isolation of nucleic acids from Chlorella is difficult, given the chemically complex nature of their cell walls and variable production of metabolites. Immobilization of microalgae in polymers adds additional difficulty. Here, we modified, amended, and standardized methods for isolation of nucleic acids and compared t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Lopez BR,Hernandez JP,Bashan Y,de-Bashan LE

    更新日期:2017-04-01 00:00:00

  • Construction of probe of the plant growth-promoting bacteria Bacillus subtilis useful for fluorescence in situ hybridization.

    abstract::Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many crops and are used as inoculants. PGPB colonization is an important trait for success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to distinguishing, by fluorescence in situ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Posada LF,Alvarez JC,Hu CH,de-Bashan LE,Bashan Y

    更新日期:2016-09-01 00:00:00

  • Building a molecular Listeria monocytogenes database to centralize and share PFGE typing data from food, environmental and animal strains throughout Europe.

    abstract::The European Union Reference Laboratory (EURL) for Listeria monocytogenes (Lm) collaborates with a network of 35 National Reference Laboratories (NRLs) throughout Europe. Most of these NRLs are in charge of detecting and typing Lm strains from food, environment and animals, which are isolated nationally. The past few ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Félix B,Danan C,Van Walle I,Lailler R,Texier T,Lombard B,Brisabois A,Roussel S

    更新日期:2014-09-01 00:00:00

  • Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments.

    abstract::Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: O'Sullivan LA,Webster G,Fry JC,Parkes RJ,Weightman AJ

    更新日期:2008-12-01 00:00:00

  • Towards a unified system for detecting waterborne pathogens.

    abstract::Currently, there is no single method to collect, process, and analyze a water sample for all pathogenic microorganisms of interest. Some of the difficulties in developing a universal method include the physical differences between the major pathogen groups (viruses, bacteria, protozoa), efficiently concentrating large...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Straub TM,Chandler DP

    更新日期:2003-05-01 00:00:00

  • Detection of an alkene monooxygenase in vinyl chloride-oxidizing bacteria with GeneFISH.

    abstract::Fluorescence in situ hybridization (FISH) can provide information on the morphology, spatial arrangement, and local environment of individual cells enabling the investigation of intact microbial communities. GeneFISH uses polynucleotide probes and enzymatic signal amplification to detect genes that are present in low ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Richards PM,Mattes TE

    更新日期:2021-01-22 00:00:00

  • A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica.

    abstract::Development of a high-throughput eukaryotic screening procedure is important to increase success in obtaining improved enzymes through directed enzyme evolution. This procedure was developed for the yeast Yarrowia lipolytica which becomes the second eukaryotic host for this purpose. The extracellular lipase Lip2 was u...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Bordes F,Fudalej F,Dossat V,Nicaud JM,Marty A

    更新日期:2007-09-01 00:00:00

  • Strain discrimination among B. anthracis and related organisms by characterization of bclA polymorphisms using PCR coupled with agarose gel or microchannel fluidics electrophoresis.

    abstract::The bclA gene codes for the protein backbone of the exosporium glycoprotein BclA of B. anthracis. BclA has a central collagen-like region formed by polymorphic GXX repeats and conserved amino- and carboxy-termini. It is noted here that the bclA gene is also present in the genome of Bacillus cereus and Bacillus thuring...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Castanha ER,Swiger RR,Senior B,Fox A,Waller LN,Fox KF

    更新日期:2006-01-01 00:00:00

  • Evaluation of RNA degradation in pure culture and field Microcystis samples preserved with various treatments.

    abstract::RNA-based molecular technique (RT-qPCR) is a promising method for microcystin monitoring in lakes and reservoirs, but great lability of RNA in cyanobacterial samples limits its application. To date, no studies have investigated how to effectively preserve RNA in cyanobacterial samples. In this study, four different tr...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Li X,Qiu D,Chen S,Li J,Luo C,Hu D,Li J,Zhu J,Chen H,Li S,Yu X

    更新日期:2019-09-01 00:00:00

  • A simple and rapid method of bacterial transformation.

    abstract::Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered susp...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Wilharm G,Lepka D,Faber F,Hofmann J,Kerrinnes T,Skiebe E

    更新日期:2010-02-01 00:00:00

  • A custom amplicon sequencing approach to detect resistance associated mutations and sequence types in Mycoplasma genitalium.

    abstract:BACKGROUND:Mycoplasma genitalium resistance to antibiotic treatments is increasing, with very limited treatment alternatives on the horizon. Surveillance via sequencing of multiple M. genitalium loci would allow: monitoring of known antibiotic resistance mutations, associations between resistance/treatment failure and ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Plummer EL,Murray GL,Bodiyabadu K,Su J,Garland SM,Bradshaw CS,Read TRH,Tabrizi SN,Danielewski JA

    更新日期:2020-12-01 00:00:00

  • Adherence and viability of intestinal bacteria to differentiated Caco-2 cells quantified by flow cytometry.

    abstract::Recent developments in host-microbe research give rise to a growing demand for rapid and accurate methods to quantify bacterial adhesion to epithelial cells. Here, we describe a new flow cytometric method to determine the amount and viability of gut bacteria, adhered to a monolayer of differentiated cells. The latter ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Grootaert C,Boon N,Zeka F,Vanhoecke B,Bracke M,Verstraete W,Van de Wiele T

    更新日期:2011-07-01 00:00:00

  • A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material.

    abstract::This study investigated the various commercially available kits and 'in-house' methods to extract DNA from Gram-negative and Gram-positive bacteria, yeast and fungal agents in commonly employed blood culture material. The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template prepar...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Millar BC,Jiru X,Moore JE,Earle JA

    更新日期:2000-10-01 00:00:00

  • Rapid identification of Armillaria species by PCR-DGGE.

    abstract::The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armil...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Longa CM,La Porta N

    更新日期:2014-12-01 00:00:00