Abstract:
:A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Santos CS,Ramos JN,Vieira VV,Pinheiro CS,Meyer R,Alcantara-Neves NM,Ramos RT,Silva A,Hirata R Jr,Felicori L,de Alegría Puig CR,Navas J,Azevedo V,Mattos-Guaraldi AL,Pacheco LGCdoi
10.1016/j.mimet.2017.09.002subject
Has Abstractpub_date
2017-11-01 00:00:00pages
33-35eissn
0167-7012issn
1872-8359pii
S0167-7012(17)30242-7journal_volume
142pub_type
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