Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers.

Abstract:

:A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.

journal_name

J Microbiol Methods

authors

Santos CS,Ramos JN,Vieira VV,Pinheiro CS,Meyer R,Alcantara-Neves NM,Ramos RT,Silva A,Hirata R Jr,Felicori L,de Alegría Puig CR,Navas J,Azevedo V,Mattos-Guaraldi AL,Pacheco LGC

doi

10.1016/j.mimet.2017.09.002

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

33-35

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(17)30242-7

journal_volume

142

pub_type

杂志文章
  • Effects of humic substances on fluorometric DNA quantification and DNA hybridization.

    abstract::DNA extracts from sediment and water samples are often contaminated with coextracted humic-like impurities. Estuarine humic substances and vascular plant extract were used to evaluate the effect of the presence of such impurities on DNA hybridization and quantification. The presence of humic substances and vascular pl...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(01)00296-2

    authors: Bachoon DS,Otero E,Hodson RE

    更新日期:2001-10-01 00:00:00

  • Analysis of major band of Enterobacter sakazakii by ERIC-PCR and development of a species-specific PCR for detection of Ent. sakazakii in dry food samples.

    abstract::ERIC (Enterobacterial Repetitive Intergenic Consensus)-PCR was employed to generate stable and reproductive ERIC-PCR fingerprints of Ent. sakazakii ATCC51329. Moreover, this study also cloned and sequenced a major band of Ent. sakazakii (ATCC51329) ERIC-PCR fingerprints. The major band was amplified with primer ERIC2 ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.07.012

    authors: Ye Y,Wu Q,Zhou Y,Dong X,Zhang J

    更新日期:2008-12-01 00:00:00

  • Optimizing ultracentrifugation conditions for DNA-based stable isotope probing (DNA-SIP).

    abstract::DNA-SIP (DNA-based stable isotope probing) is increasingly being employed in soil microbial ecology to identify those microbes assimilating the 13C/15N labelled substrate. Isopycnic gradient centrifugation is the primary experimental process for conducting DNA-SIP. However, diverse centrifugal conditions have been use...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.105938

    authors: Wang J,Zhang X,Yao H

    更新日期:2020-06-01 00:00:00

  • Optimization of electrotransformation (ETF) conditions in lactic acid bacteria (LAB).

    abstract::Lactic acid bacteria (LAB) play important roles in the food industry, animal husbandry and medicine which are closely related to human life. Modern gene engineering technology is the major means to reveal gene functions, study metabolic pathways and metabolomes of strains and improve the properties of strains. However...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.mimet.2020.105944

    authors: Wang C,Cui Y,Qu X

    更新日期:2020-07-01 00:00:00

  • A reverse-hybridization test for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs.

    abstract::The S. PneumoStrip test is a recently developed reverse hybridization strip-based commercial assay that allows for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs, according to their specific gene sequences. The test was validated with reference strains of 92 different pneumococcal ser...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2017.08.010

    authors: Marimón JM,Morales M,Gamen S,Manrique A,Ercibengoa M,Cilla G

    更新日期:2017-12-01 00:00:00

  • The identification of carbapenemase types in Enterobacteriaceae by using molecular assay and phenotyping confirmation tests.

    abstract::In this study, we aimed to identify the molecular carbapenemase types of the Enterobacteriaceae isolates and to evaluate the performance of manually prepared and commercially available combination disc methods and the modified Hodge test. One hundred and forty carbapenemase producing isolates and 45 isolates as contro...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.03.010

    authors: Genc O,Aksu E,Gulcan A

    更新日期:2016-06-01 00:00:00

  • A genomic library-based amplification approach (GL-PCR) for the mapping of multiple IS6110 insertion sites and strain differentiation of Mycobacterium tuberculosis.

    abstract::Evidence suggests that insertion of the IS6110 element is not without consequence to the biology of Mycobacterium tuberculosis complex strains. Thus, mapping of multiple IS6110 insertion sites in the genome of biomedically relevant clinical isolates would result in a better understanding of the role of this mobile ele...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2006.03.021

    authors: Namouchi A,Mardassi H

    更新日期:2006-11-01 00:00:00

  • A novel CMOS image sensor system for quantitative loop-mediated isothermal amplification assays to detect food-borne pathogens.

    abstract::Loop-mediated isothermal amplification (LAMP) is considered as one of the alternatives to the conventional PCR and it is an inexpensive portable diagnostic system with minimal power consumption. The present work describes the application of LAMP in real-time photon detection and quantitative analysis of nucleic acids ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.12.002

    authors: Wang T,Kim S,An JH

    更新日期:2017-02-01 00:00:00

  • A toolbox to measure changes in the cell wall glycopolymer composition during differentiation of Streptomyces coelicolor A3(2).

    abstract::Cell wall glycopolymers (CWG) represent an important component of the Gram-positive cell envelope with many biological functions. The mycelial soil bacterium Streptomyces coelicolor A3(2) incorporates two distinct CWGs, polydiglycosylphosphate (PDP) and teichulosonic acid, into the cell wall of its vegetative mycelium...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.07.002

    authors: Sigle S,Steblau N,Wohlleben W,Muth G

    更新日期:2016-09-01 00:00:00

  • Rapid antibiotic susceptibility phenotypic characterization of Staphylococcus aureus using automated microscopy of small numbers of cells.

    abstract::Staphylococcus aureus remains a leading, virulent pathogen capable of expressing complex drug resistance that requires up to 2-4 days for laboratory analysis. In this study, we evaluate the ability of automated microscopy of immobilized live bacterial cells to differentiate susceptible from non-susceptible responses o...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2013.12.021

    authors: Price CS,Kon SE,Metzger S

    更新日期:2014-03-01 00:00:00

  • Impact of sampling and DNA extraction methods on skin microbiota assessment.

    abstract::The skin microbiota is characterized by high intra- and inter-variability among individuals, due to a multitude of intrinsic and extrinsic parameters such as genetics, lifestyles or pollution. This variability may be heightened due to sampling method as the skin is a multilayer organ and its outermost layer consists o...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.105880

    authors: Boulesnane Y,Leloup J,Lerch TZ,Roynette A,Pensé-Lhéritier AM,Mielcarek C,Changey F

    更新日期:2020-04-01 00:00:00

  • Permeabilization and hybridization protocols for rapid detection of Bacillus spores using fluorescence in situ hybridization.

    abstract:BACKGROUND:Fluorescence in situ hybridization (FISH) is not adapted for the detection of bacterial spores because of their resistance to conventional permeabilization treatments. Since spore-forming bacteria have important ecological, economical, and medical roles, their in situ detection needs to be improved. The aim ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.12.009

    authors: Filion G,Laflamme C,Turgeon N,Ho J,Duchaine C

    更新日期:2009-04-01 00:00:00

  • An improved high throughput sequencing method for studying oomycete communities.

    abstract::Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2015.01.013

    authors: Sapkota R,Nicolaisen M

    更新日期:2015-03-01 00:00:00

  • Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining.

    abstract::An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g(-1) for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g(-1) soil. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2013.07.019

    authors: Orlofsky E,Gillor O,Melli A,Miller W,Wuertz S,Bernstein N,Shapiro K

    更新日期:2013-09-01 00:00:00

  • Wintergreen oil: a novel method in Wheatley's trichrome staining technique.

    abstract::Permanent staining of faecal smears by Wheatley's trichrome technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome staining technique...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2012.08.004

    authors: Salleh FM,Anuar TS,Yasin AM,Moktar N

    更新日期:2012-10-01 00:00:00

  • Is sonication of antibiotic-loaded cement spacers useful in two-stage revision of prosthetic joint infection?

    abstract:BACKGROUND:In a two-stage exchange protocol for prosthetic joint infection (PJI), bacteria surviving over the antibiotic-loaded cement spacers may cause the persistence of infection with renewed clinical symptoms following the surgery. Culture after sonication of removed prosthesis is more sensitive than conventional p...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2018.12.006

    authors: Sambri A,Maso A,Storni E,Donati ME,Pederzoli A,Dallari D,Bianchi G,Donati DM

    更新日期:2019-01-01 00:00:00

  • Towards a unified system for detecting waterborne pathogens.

    abstract::Currently, there is no single method to collect, process, and analyze a water sample for all pathogenic microorganisms of interest. Some of the difficulties in developing a universal method include the physical differences between the major pathogen groups (viruses, bacteria, protozoa), efficiently concentrating large...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/s0167-7012(03)00023-x

    authors: Straub TM,Chandler DP

    更新日期:2003-05-01 00:00:00

  • Detection of Cyclospora cayetanensis using a quantitative real-time PCR assay.

    abstract::Cyclospora cayetanensis, a coccidian parasite, with a fecal-oral life cycle, has become recognized worldwide as an emerging human pathogen. Clinical manifestations include prolonged gastroenteritis. While most cases of infection with C. cayetanensis in the United States have been associated with foodborne transmission...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(02)00209-9

    authors: Varma M,Hester JD,Schaefer FW 3rd,Ware MW,Lindquist HD

    更新日期:2003-04-01 00:00:00

  • Actinomyces naeslundii GroEL-dependent initial attachment and biofilm formation in a flow cell system.

    abstract::Actinomyces naeslundii is an early colonizer with important roles in the development of the oral biofilm. The effects of butyric acid, one of short chain fatty acids in A. naeslundii biofilm formation was observed using a flow cell system with Tryptic soy broth without dextrose and with 0.25% sucrose (TSB sucrose). Si...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2014.12.021

    authors: Arai T,Ochiai K,Senpuku H

    更新日期:2015-02-01 00:00:00

  • Development of a method for heterologous gene expression in Enterobacter amnigenus, a potential host for the biological control of mosquito larvi.

    abstract::An integrative plasmid containing a 1.3 kb fragment of chromosomal DNA from Enterobacter amnigenus was constructed. The Omega fragment encoding spectinomycin/streptomycin resistance was cloned into the unique BglII site of the resulting plasmid, and the interrupted fragment was transferred via plasmid pMAK705 by elect...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(01)00384-0

    authors: Nantapong N,Tanapongpipat S,Cole J,Panyim S

    更新日期:2002-05-01 00:00:00

  • Development of a multiplex PCR assay for the detection of major virulence genes in Vibrio cholerae including non-O1 and non-O139 serogroups.

    abstract::Vibrio cholerae strains producing cholera toxin (CT) and toxin co-regulated pilus (TCP) and belonging to O1 and O139 serogroups are responsible for cholera. However, non-CT producing V. cholerae from non-O1/non-O139 serogroups have been increasingly isolated from diarrheal stools and extra-intestinal infections. In th...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2018.12.012

    authors: Awasthi SP,Chowdhury N,Neogi SB,Hinenoya A,Hatanaka N,Chowdhury G,Ramamurthy T,Yamasaki S

    更新日期:2019-02-01 00:00:00

  • An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira.

    abstract::Thanks to their photosynthetic and nutritive properties, cyanobacteria of the Arthrospira genus are of interest as food supplements, as efficient oxygen producing life support system organisms for manned space flight, and for the production of biofuels. Despite these potential valuable applications, full genome sequen...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2009.11.012

    authors: Morin N,Vallaeys T,Hendrickx L,Natalie L,Wilmotte A

    更新日期:2010-02-01 00:00:00

  • Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR.

    abstract::Four different bacterial DNA extraction strategies and two different qPCR probe chemistries were studied for detection of Stapylococcus aureus from white cheeses. Method employing trypsin treatment followed by a commercial kit application and TaqMan probe-based qPCR was the most sensitive one detecting higher counts t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2014.06.022

    authors: Kadiroğlu P,Korel F,Ceylan C

    更新日期:2014-10-01 00:00:00

  • A simple, rapid, sensitive method detecting homoserine lactone (HSL)-related compounds in microbial extracts.

    abstract::A simple, rapid, sensitive microtiter plate method detecting N-acyl homoserine lactone (HSL)-related compounds was established using an Agrobacterium tumefaciens strain harboring a traG::lacZ/traR reporter gene responsive to HSLs. This strain did not produce its own HSL, but the traG::lacZ reporter gene was induced on...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2005.06.003

    authors: Singh MP,Greenstein M

    更新日期:2006-04-01 00:00:00

  • Immobilization in alginate as a technique for the preservation of Bacillus thuringiensis var. israelensis for long-term preservation.

    abstract::Technique for immobilization using sodium alginate as the matrix to preserve Bacillus thuringiensis var. israelensis isolates for long time storage was developed. Two strains of B. thuringiensis var. israelensis viz., VCRC B-17 and WHO standard strain IPS-82 were immobilized in alginate matrix and preserved at 4 degre...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2007.11.001

    authors: Prabakaran G,Hoti SL

    更新日期:2008-01-01 00:00:00

  • Relating growth dynamics and glucoamylase excretion of individual Saccharomyces cerevisiae cells.

    abstract::We have developed a novel flow cytometric procedure that allows determinations of properties of protein excretion in the growth medium on a cell-by-cell basis in Saccharomyces cerevisiae. The procedure is based on labelling of a periplasmically secreted protein with antibodies conjugated to a fluorescent marker such a...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(00)00171-8

    authors: Porro D,Venturini M,Brambilla L,Alberghina L,Vanoni M

    更新日期:2000-09-01 00:00:00

  • Correction pen based paper fluidic device for the detection of multiple gene targets of Leptospira using Loop Mediated Isothermal Amplification.

    abstract::Paper-based nucleic acid testing techniques are increasingly in demand. Hence, we have developed a simple and cheap paper fluidic device to detect multiple gene targets in Leptospira. Fluidic channels of the penta-clover device are drawn using a correction pen on Whatman filter paper 1. The fluid blocks the pores of t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.105962

    authors: Varsha V,Aishwarya S,Murchana S,Naveen G,Ramya M,Rathinasabapathi P

    更新日期:2020-07-01 00:00:00

  • Strain discrimination among B. anthracis and related organisms by characterization of bclA polymorphisms using PCR coupled with agarose gel or microchannel fluidics electrophoresis.

    abstract::The bclA gene codes for the protein backbone of the exosporium glycoprotein BclA of B. anthracis. BclA has a central collagen-like region formed by polymorphic GXX repeats and conserved amino- and carboxy-termini. It is noted here that the bclA gene is also present in the genome of Bacillus cereus and Bacillus thuring...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2005.04.032

    authors: Castanha ER,Swiger RR,Senior B,Fox A,Waller LN,Fox KF

    更新日期:2006-01-01 00:00:00

  • A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential.

    abstract::We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2010.08.012

    authors: Gentry DR,Wilding I,Johnson JM,Chen D,Remlinger K,Richards C,Neill S,Zalacain M,Rittenhouse SF,Gwynn MN

    更新日期:2010-11-01 00:00:00

  • Quantification of bacterial lead resistance via activity assays.

    abstract::The level of microbial resistance to heavy metals is an important issue for the microbial ecology of heavy metal-contaminated habitats. However, assays based upon growth in nutrient media will overestimate the resistance level due to metal ion interactions with inorganic and organic components. The analysis of Pb-resi...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(99)00032-9

    authors: Konopka A,Zakharova T

    更新日期:1999-07-01 00:00:00