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Analysis of the genetic distribution among members of Clostridium botulinum group I using a novel multilocus sequence typing (MLST) assay.

Abstract：

:Clostridium botulinum is the etiological agent of botulism. Due to food-borne poisoning and the potential use of the extremely toxic botulinum neurotoxin (BoNT) from C. botulinum in bioterror or biocrime related actions, reliable high resolution typing methods for discriminating C. botulinum strains are needed. Partial sequencing of the adk, atpH, gyrB, proC, rpoD and spo0A genes from 51 various C. botulinum/sporogenes isolates was performed, resulting in 37 different sequence types (STs). Analysis of the sequence data revealed a genetic distribution in five larger clusters with a loose correlation to the BoNT serotypes. The developed MLST assay had a slightly lower resolution ability when compared to the MLVA (multilocus variable number of tandem repeat analysis), but the two methods resulted in similar subclusters of the strains possessing the BoNT serotypes A, B and F. The current work presents the development of a novel MLST assay useful for genotyping C. botulinum related to basic phylogenetic research and trace-back analysis in microbial forensic studies.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Olsen JS,Scholz H,Fillo S,Ramisse V,Lista F,Trømborg AK,Aarskaug T,Thrane I,Blatny JM

doi

10.1016/j.mimet.2013.11.003

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

84-91

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(13)00341-2

journal_volume

96

pub_type

杂志文章

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