Abstract:
:Novel noninvasive techniques for the removal of biological contaminants to generate clean or sterile materials are in demand by the medical, pharmaceutical and food industries. The sterilization method described here uses supercritical fluid carbon dioxide (SF-CO(2)) containing 3.3% water and 0.1% hydrogen peroxide (v/v/v) to achieve from four to eight log viability reduction of all tested microbial species, including vegetative cells, spores and biofilms. The sterilization method employs moderate pressure and temperature (80 atm, 50°C) and a short (30-minute) treatment time. The procedure kills various opportunistic pathogens that often persist in biofilm structures, fungal spores commonly associated with nosocomial infections, and Bacillus pumilus SAFR-032 endospores that are notoriously hard to eradicate by conventional sterilization techniques.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Checinska A,Fruth IA,Green TL,Crawford RL,Paszczynski AJdoi
10.1016/j.mimet.2011.07.008subject
Has Abstractpub_date
2011-10-01 00:00:00pages
70-5issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(11)00258-2journal_volume
87pub_type
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