A new chromogenic agar medium for detection of potentially virulent Yersinia enterocolitica.


:Several outbreaks of foodborne yersiniosis have been documented and this disease continues to be source of infections transmitted through foods. The selective agars most commonly used to isolate Yersinia enterocolitica in clinical, food and environmental samples, cefsulodin-irgasan-novobiocin (CIN) and MacConkey (MAC) agars, lack the ability to differentiate potentially virulent Y. enterocolitica from other Yersinia that may be present as well as some other bacterial spp. This study proposes the use of an agar medium, Y. enterocolitica chromogenic medium (YeCM), for isolation of potentially virulent Y. enterocolitica. This agar contains cellobiose as the fermentable sugar, a chromogenic substrate and selective inhibitors for suppression of colony formation by many competing bacteria. All strains of potentially virulent Yersinia of biotypes 1B, and biotypes 2-5 formed convex, red bulls-eye colonies on YeCM that were very similar to those described for CIN agar. However, Y. enterocolitica biotype 1A and other related Yersinia formed colonies that were purple/blue on YeCM while they formed typical red bulls-eye colonies on CIN agar. When a mixture of potentially virulent Y. enterocolitica biotype 1B, Y. enterocolitica biotype 1A and 5 other bacterial species was used to artificially contaminate tofu and then spread-plated on three selective agars, Y. enterocolitica biotype 1B colonies were easily distinguished from other strains on YeCM. However, Y. enterocolitica biotype 1B colonies were indistinguishable from many other colonies on CIN and only distinguishable from those of C. freundii on MAC. When colonies were picked and identified from these agars, typical colonies from YeCM were confirmed only as Y. enterocolitica biotype 1B. Typical colonies on CIN and MAC were found to belong to several competing species and biotypes.


J Microbiol Methods


Weagant SD




Has Abstract


2008-02-01 00:00:00














  • A preliminary evaluation of a new enzyme immunoassay to detect Chlamydia pneumoniae-specific antibodies.

    abstract::New enzyme immunoassays (EIAs) for determination of specific IgG, IgA, and IgM antibody titers to Chlamydia pneumoniae were evaluated independently in three research laboratories. Specificity of the EIAs was enhanced by removing LPS from the chlamydial antigen. The performance of these EIAs was evaluated in comparison...

    journal_title:Journal of microbiological methods

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Ossewaarde JM,Tuuminen T,Boersma WG,Sandström M,Palomäki P,Boman J

    更新日期:2000-12-15 00:00:00

  • Toward an international standard for PCR-based detection of Escherichia coli O157. Part 1. Assay development and multi-center validation.

    abstract::As part of a major European research project, a diagnostic PCR assay, including an internal amplification control, was developed and validated in a collaborative trial for the detection of Escherichia coli O157. The assay is based on amplification of sequences of the rfbE O157 gene. The collaborative trial, including ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,多中心研究


    authors: Abdulmawjood A,Bülte M,Cook N,Roth S,Schönenbrücher H,Hoorfar J

    更新日期:2003-12-01 00:00:00

  • Evaluation of Ag containing hydroxyapatite coatings to the Candida albicans infection.

    abstract::In this research work, the synthesis of Ag doped hydroxyapatite coatings for dental or orthopedic implants was performed. The main goal was to determine the influence of Ag content on the roughness and antimicrobial performance of the prepared thin films. The films were deposited on Ti6Al4V alloy by means of RF magnet...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Ciuca S,Badea M,Pozna E,Pana I,Kiss A,Floroian L,Semenescu A,Cotrut CM,Moga M,Vladescu A

    更新日期:2016-06-01 00:00:00

  • A novel method for the measurement of dissolved adenosine and guanosine triphosphate in aquatic habitats: applications to marine microbial ecology.

    abstract::A novel method for the measurement of dissolved adenosine-5'-triphosphate and guanosine-5'-triphosphate (D-ATP and D-GTP, respectively) in marine and freshwater habitats was developed and applied to samples collected from the oligotrophic North Pacific Ocean. Both D-ATP and D-GTP are co-precipitated by authigenically ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Björkman KM,Karl DM

    更新日期:2001-11-01 00:00:00

  • Methods used for the detection and subtyping of Listeria monocytogenes.

    abstract::Listeria monocytogenes is an important foodborne pathogen responsible for non-invasive and invasive diseases in the elderly, pregnant women, neonates and immunocompromised populations. This bacterium has many similarities with other non-pathogenic Listeria species which makes its detection from food and environmental ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Jadhav S,Bhave M,Palombo EA

    更新日期:2012-03-01 00:00:00

  • Development and standardization of an in-house indirect ELISA for detection of duck antibody to fowl cholera.

    abstract::Serological tests, such as agglutination and indirect hemagglutination assay (IHA), have been used to identify antibodies against Pasteurella multocida in poultry sera, but none are highly sensitive. An enzyme-linked immunosorbent assays (ELISA) has been used with varying degrees of success in attempts to monitor sero...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Poolperm P,Varinrak T,Kataoka Y,Tragoolpua K,Sawada T,Sthitmatee N

    更新日期:2017-11-01 00:00:00

  • An improved high throughput sequencing method for studying oomycete communities.

    abstract::Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sapkota R,Nicolaisen M

    更新日期:2015-03-01 00:00:00

  • Isolation of Brucella abortus total RNA from B. abortus-infected murine RAW macrophages.

    abstract::Brucella is a Gram-negative facultative bacterium that persists intracellularly in macrophages. However, the intracellular survival mechanisms used by Brucella are not fully understood. Isolation of Brucella RNA from infected macrophages has been challenging, and the inability to isolate sufficient Brucella RNA from i...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Covert J,Eskra L,Splitter G

    更新日期:2005-03-01 00:00:00

  • An array of multiplex PCR assays for detection of staphylococcal chromosomal cassette mec (SCCmec) types among staphylococcal isolates.

    abstract::Staphylococcal Cassette Chromosome mec (SCCmec) element plays a key role in development of resistance by carrying different resistance factors. Therefore, routine and rapid diagnostic approach is considered advantageous for the easy detection of SCCmec elements. So, here we have described the use of three sets of mult...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Bhowmik D,Das BJ,Pandey P,Chetri S,Chanda DD,Bhattacharjee A

    更新日期:2019-11-01 00:00:00

  • Development and validation of evolutionary algorithm software as an optimization tool for biological and environmental applications.

    abstract::A flexible, extendable tool for the optimization of (micro)biological processes and protocols using evolutionary algorithms was developed. It has been tested using three different theoretical optimization problems: 2 two-dimensional problems, one with three maxima and one with five maxima and a river autopurification ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sys K,Boon N,Verstraete W

    更新日期:2004-06-01 00:00:00

  • Suppression subtractive hybridisation and real-time PCR for strain-specific quantification of the probiotic Bifidobacterium animalis BAN in broiler feed.

    abstract::To ensure quality management during the production processes of probiotics and for efficacy testing in vivo, accurate tools are needed for the identification and quantification of probiotic strains. In this study, a strain-specific qPCR assay based on Suppression Subtractive Hybridisation (SSH) for identifying unique ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Fibi S,Klose V,Mohnl M,Weber B,Haslberger AG,Sattler VA

    更新日期:2016-04-01 00:00:00

  • Optimization of electrotransformation (ETF) conditions in lactic acid bacteria (LAB).

    abstract::Lactic acid bacteria (LAB) play important roles in the food industry, animal husbandry and medicine which are closely related to human life. Modern gene engineering technology is the major means to reveal gene functions, study metabolic pathways and metabolomes of strains and improve the properties of strains. However...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Wang C,Cui Y,Qu X

    更新日期:2020-07-01 00:00:00

  • Genotypic characterization of Salmonella by multilocus sequence typing, pulsed-field gel electrophoresis and amplified fragment length polymorphism.

    abstract::Molecular typing is an important tool in surveillance and outbreak investigations of human Salmonella infections. In this study, three molecular typing methods were used to investigate the discriminatory ability, reproducibility and the genetic relationship between 110 Salmonella enterica subspecies enterica isolates....

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Torpdahl M,Skov MN,Sandvang D,Baggesen DL

    更新日期:2005-11-01 00:00:00

  • Identification and profiling of volatile metabolites of the biocontrol fungus Trichoderma atroviride by HS-SPME-GC-MS.

    abstract::In the present study we describe a method, which is based on solid phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS) and which can be used for the profiling of microbial volatile organic compounds (MVOCs) in the headspace (HS) of cultures of filamentous fungi. The method comprises th...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Stoppacher N,Kluger B,Zeilinger S,Krska R,Schuhmacher R

    更新日期:2010-05-01 00:00:00

  • Improving the selection efficiency of the counter-selection marker pheS* for the genetic engineering of Bacillus amyloliquefaciens.

    abstract::Bacillus subtilis pheS was genetically modified to obtain a counter-selection marker with high selection efficiency in Bacillus amyloliquefaciens. The application of the new replication-thermosensitive integrative vector pNZTM1, containing this marker, pheSBsT255S/A309G, with a two-step replacement recombination proce...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Kharchenko MS,Teslya PN,Babaeva MN,Zakataeva NP

    更新日期:2018-05-01 00:00:00

  • Molecular detection and quantification of viable probiotic strains in animal feedstuffs using the commercial direct fed microbial Lactobacillus animalis NP51 as a model.

    abstract::Lactobacillus animalis NP51 is a direct-fed microbial strain (DFM) extensively used as a pre-harvest food safety mitigation in feedlot cattle due to its antagonistic effects against human foodborne pathogens such as Salmonella and Escherichia coli O157:H7. NP51 not only promotes overall gut health but interferes with ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Ayala DI,Chen JC,Bugarel M,Loneragan GH,den Bakker HC,Kottapalli KR,Brashears MM,Nightingale KK

    更新日期:2018-06-01 00:00:00

  • An IC-PCR method for detection of Cryptosporidium and Giardia in natural surface waters in Finland.

    abstract::We developed an immunocapture-based polymerase chain reaction (PCR) assay for simultaneous detection of Cryptosporidium parvum oocysts and Giardia intestinalis cysts in surface water. Using primer pairs Cry9/Cry15 and LaxA/LaxB for Cryptosporidium and Gdh1/Gdh4 for Giardia, the sensitivity of the entire detection proc...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Rimhanen-Finne R,Hörman A,Ronkainen P,Hänninen ML

    更新日期:2002-08-01 00:00:00

  • Comparative assessment of next-generation sequencing, denaturing gradient gel electrophoresis, clonal restriction fragment length polymorphism and cloning-sequencing as methods for characterizing commercial microbial consortia.

    abstract::Characterization of commercial microbial consortia products for human and environmental health risk assessment is a major challenge for regulatory agencies. As a means to develop an approach to assess the potential environmental risk of these products, research was conducted to compare four genomics methods for charac...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Samarajeewa AD,Hammad A,Masson L,Khan IU,Scroggins R,Beaudette LA

    更新日期:2015-01-01 00:00:00

  • A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material.

    abstract::This study investigated the various commercially available kits and 'in-house' methods to extract DNA from Gram-negative and Gram-positive bacteria, yeast and fungal agents in commonly employed blood culture material. The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template prepar...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Millar BC,Jiru X,Moore JE,Earle JA

    更新日期:2000-10-01 00:00:00

  • Live imaging of the genetically intractable obligate intracellular bacteria Orientia tsutsugamushi using a panel of fluorescent dyes.

    abstract::Our understanding of the molecular mechanisms of bacterial infection and pathogenesis are disproportionally derived from a small number of well-characterised species and strains. One reason for this is the enormous time and resources required to develop a new organism into experimental system that can be interrogated ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Atwal S,Giengkam S,VanNieuwenhze M,Salje J

    更新日期:2016-11-01 00:00:00

  • Optimal methods for evaluating antimicrobial activities from plant extracts.

    abstract::The search for antimicrobial agents from plants has been a growing interest in the last few decades. However, results generated from many of these studies cannot be directly compared due to the absence of standardization in particular antimicrobial methods employed. The need for established methods with consistent res...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Othman M,Loh HS,Wiart C,Khoo TJ,Lim KH,Ting KN

    更新日期:2011-02-01 00:00:00

  • Actinomyces naeslundii GroEL-dependent initial attachment and biofilm formation in a flow cell system.

    abstract::Actinomyces naeslundii is an early colonizer with important roles in the development of the oral biofilm. The effects of butyric acid, one of short chain fatty acids in A. naeslundii biofilm formation was observed using a flow cell system with Tryptic soy broth without dextrose and with 0.25% sucrose (TSB sucrose). Si...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Arai T,Ochiai K,Senpuku H

    更新日期:2015-02-01 00:00:00

  • Validation of DNA and RNA real-time assays for food analysis using the hilA gene of Salmonella enterica serovars.

    abstract::In Europe, alternative methods for the detection of food-borne pathogens can be used instead of the standard ISO/CEN reference protocol, if validated according to the protocol outlined in ISO 16140, 2003. In this study, the performance of two novel methods for the detection of Salmonella sp. using real-time PCR techno...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: McCabe EM,Burgess CM,Walsh D,O'Regan E,McGuinness S,Barry T,Fanning S,Duffy G

    更新日期:2011-01-01 00:00:00

  • Hydrophysical correlation and water mass indication of optical physiological parameters of picophytoplankton in Prydz Bay during autumn 2008.

    abstract::Flow cytometry (FCM) is efficient in detecting both abundance and optical physiological parameters including cell size and cellular carbon content-side scatter (SSC), carotenoids-green and orange fluorescence (FL1 and FL2), and red fluorescence-chlorophylls (FL3) can be obtained by FCM. The utilization of these physio...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Zhang F,Ma Y,Lin L,He J

    更新日期:2012-12-01 00:00:00

  • Adhesion of Yersinia enterocolitica to non-cultured epithelial cells from pig and rabbit ilea.

    abstract::A simple and reliable method was developed to isolate intact epithelial cells from pig and rabbit ilea and these were used to investigate the adhesion of Yersinia enterocolitica. Hydrophobic interaction was eliminated by treating the bacterial culture with 0.8 M tetramethyl urea (TMU). Virulent strains of Y. enterocol...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Hussein HM,Fenwick SG,Lumsden JS

    更新日期:2007-02-01 00:00:00

  • Adherence and viability of intestinal bacteria to differentiated Caco-2 cells quantified by flow cytometry.

    abstract::Recent developments in host-microbe research give rise to a growing demand for rapid and accurate methods to quantify bacterial adhesion to epithelial cells. Here, we describe a new flow cytometric method to determine the amount and viability of gut bacteria, adhered to a monolayer of differentiated cells. The latter ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Grootaert C,Boon N,Zeka F,Vanhoecke B,Bracke M,Verstraete W,Van de Wiele T

    更新日期:2011-07-01 00:00:00

  • Application of Cytosense flow cytometer for the analysis of airborne bacteria collected by a high volume impingement sampler.

    abstract::Characterization of airborne bacterial cells requires efficient collection, concentration, and analysis techniques, particularly to overcome the challenge of their dilute nature in outdoor environments. This study aims to establish a rapid and reliable approach for quantification of bacteria in air samples. To do this...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Jang J,Hendriksen NB,Jakobsen HH,Gosewinkel U

    更新日期:2018-11-01 00:00:00

  • Real-time quantitative PCR detection of Mycobacterium avium subsp. paratuberculosis and differentiation from other mycobacteria using SYBR Green and TaqMan assays.

    abstract::Sensitive real-time sequence detection methods based on two different chemistries were developed for Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of Johne's disease in cattle. One is based on the detection of SYBR Green bound to PCR products and the second method is more specific, detecting t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Ravva SV,Stanker LH

    更新日期:2005-12-01 00:00:00

  • Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments.

    abstract::Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: O'Sullivan LA,Webster G,Fry JC,Parkes RJ,Weightman AJ

    更新日期:2008-12-01 00:00:00

  • Application of green fluorescent protein to measure antimicrobial efficacy and the kinetics of cell death against Escherichia coli.

    abstract::Industrial antimicrobials have been extensively used to control unwanted microbial growth by incorporation into a variety of products such as plastics and paints, reducing biodeterioration and biofouling and extending the lifespan of the product. Industrial antimicrobials generally have broad sites of action affecting...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Greenhalgh R,Greenhalgh M,Alshareef F,Robson GD

    更新日期:2017-10-01 00:00:00