Abstract：
:Aggregatibacter (A.) actinomycetemcomitans is a periodontopathogenic bacterium causing aggressive periodontitis. Here we describe a single-step transconjugation system as novel and easily applicable protocol for site-specific genetic manipulation of A. actinomycetemcomitans. Deletion of PgaC, which is involved in the synthesis of biofilm matrix, led to a reduced biofilm formation.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Stumpp NS,Konze SA,Gerardy-Schahn R,Stiesch M,Buettner FFRdoi
10.1016/j.mimet.2018.04.002subject
Has Abstractpub_date
2018-05-01 00:00:00pages
74-77eissn
0167-7012issn
1872-8359pii
S0167-7012(18)30084-8journal_volume
148pub_type
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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