当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > A comparative study of biofilm formation by Shiga toxigenic Escherichia coli using epifluorescence microscopy on stainless steel and a microtitre plate method.

A comparative study of biofilm formation by Shiga toxigenic Escherichia coli using epifluorescence microscopy on stainless steel and a microtitre plate method.

Abstract：

:Attachment of Shiga toxigenic Escherichia coli (STEC) to surfaces and the formation of biofilms may enhance persistence in a food processing environment and present a risk of contaminating products. Seven strains of STEC and three non-STEC strains were selected to compare two biofilm quantification methods; epifluorescence microscopy on stainless steel (SS) and a microtitre plate assay. The influence of prior growth in planktonic (nutrient broth) and sessile (nutrient agar) culture on biofilm production, as well as expression of surface structures and the possession of antigen 43 (encoded by agn43) on biofilm formation were also investigated. Biofilms were produced in diluted nutrient broth at 25 degrees C for 24 and 48 h. Curli expression was determined using congo red indicator agar, while the presence of agn43 was determined using polymerase chain reaction. No correlation was found between counts for epifluorescence microscopy on SS and the absorbance values obtained with the microtitre plate method for planktonic and sessile grown cultures. Different abilities of individual STEC strains to attach to SS and microtitre plates were found with some strains attaching better to each surface following growth in either planktonic or sessile culture. All O157 STEC strains had low biofilm counts on SS for planktonic and sessile grown cultures; however, one STEC O157:H- strain (EC516) had significantly greater (p<0.05) biofilm production on microtitre plates compared to the other O157 STEC strains. EC516 and other STEC (O174:H21 and O91:H21) strains expressing curli fimbriae were found to produce significantly greater (p<0.05) biofilms on microtitre plates compared to the non-curli expressing strains. No relationship was found between the production of type-I fimbriae, motility, agn43 and bacterial physicochemical properties (previously determined) and biofilm formation on SS or microtitre plates. Variations between the two biofilm determination methods may suggest that the biofilm production on microtitre plates may not be appropriate to represent other surfaces such as SS and that caution should be taken when selecting a method to quantify biofilm production on a surface.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Rivas L,Dykes GA,Fegan N

doi

10.1016/j.mimet.2006.11.014

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

44-51

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(06)00344-7

journal_volume

69

pub_type

杂志文章

相关文献

JOURNAL OF MICROBIOLOGICAL METHODS文献大全
  • WITHDRAWN: Multiplex particle refining system to enhance Cryptosporidium recovery for surface water filtration methods.

    abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.org/doi:10.1016/j.mimet.2012.12.009. The duplicate article has therefore been withdrawn. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.12.009

    authors： Pires NM,Dong T

    更新日期：2012-12-20 00:00:00

  • Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells.

    abstract：:A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strain...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.01.021

    authors： Matsuoka H,Shigetomi T,Funabashi H,Saito M,Igimi S

    更新日期：2013-04-01 00:00:00

  • Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds.

    abstract：:A real-time quantitative polymerase chain reaction (Q-PCR) assay was developed for detecting and quantifying Escherichia coli in water samples from agricultural watersheds. The assay included optimization of DNA extraction and purification from water samples, and Q-PCR amplification conditions using newly designed spe...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.02.016

    authors： Khan IU,Gannon V,Kent R,Koning W,Lapen DR,Miller J,Neumann N,Phillips R,Robertson W,Topp E,van Bochove E,Edge TA

    更新日期：2007-06-01 00:00:00

  • Development of a simple root model to study the effects of single exudates on the development of bacterial community structure.

    abstract：:The plant root interface is a hot spot for microbial activities. Root exudates are the key compounds that drive microbial performance. However quality and amount of root exudates are highly dynamic in time and space, thus a direct influence of a single compound on a microbial community composition is fairly impossible...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.04.003

    authors： Ziegler M,Engel M,Welzl G,Schloter M

    更新日期：2013-07-01 00:00:00

  • A novel and convenient self-drying system for bacterial preservation.

    abstract：:An effective microbial preservation technology for the long-term storage of viable prokaryotic cells is described. The method combines an almost instantaneous drying step with minimal stress to the cells during drying to maximize survival on rehydration. This is achieved by contact of a microlitre aliquot of a bacteri...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.02.014

    authors： Hays HC,Millner PA,Jones JK,Rayner-Brandes MH

    更新日期：2005-10-01 00:00:00

  • A novel 96-well gel-based assay for determining antifungal activity against filamentous fungi.

    abstract：:In recent years the global rise in antibiotic resistance and environmental consciousness lead to a renewed fervour to find and develop novel antibiotics, including antifungals. However, the influence of the environment on antifungal activity is often disregarded and many in vitro assays may cause the activity of certa...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.09.025

    authors： Troskie AM,Vlok NM,Rautenbach M

    更新日期：2012-12-01 00:00:00

  • Eco-friendly, economic fungal universal medium from watermelon peel waste.

    abstract：:Watermelon peel waste (WPW) is a zero value material containing a high amount of nutrients that could be utilized in microbial growth medium production. In the present work, WPW was used to produce universal fungal growth medium with great nutritional profile to use instead of other expensive media particularly the Po...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.105802

    authors： Hasanin MS,Hashem AH

    更新日期：2020-01-01 00:00:00

  • Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS.

    abstract：:A protein database was constructed and validated with identification rate over 90% for the 4 most common Gram-negative bacteria on agar plates. By protein masses comparison, 120 bacteria of the 4 species from blood culture bottles were identified. The concordance was high (Kappa=0.906) between our method and conventio...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.07.021

    authors： Xiao D,Yang Y,Jiang W,Zhang H,Liu H,Yu H,Xie C,Zhong M,Chen L,Huang W

    更新日期：2014-10-01 00:00:00

  • Use of denaturing gradient gel electrophoresis for analysis of the stool microbiota of hospitalized patients.

    abstract：:Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified ribosomal RNA gene amplicons was used to study the stool microbiota of hospitalized patients and to examine the effect of antibiotic therapy. For one patient, 16 anaerobic species identified by random cloning and sequencing of PCR-amplified rRNA genes fro...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(03)00059-9

    authors： Donskey CJ,Hujer AM,Das SM,Pultz NJ,Bonomo RA,Rice LB

    更新日期：2003-08-01 00:00:00

  • Long term storage of fastidious bacteria (Neisseria spp. and Haemophilus spp.) with swab preservation at -80 °C.

    abstract：:Preservation of strains is important to further studies and characterization; we have shown a technique of long-term preservation of Neisseria spp. and Haemophilus spp. by freezing cotton tip swabs impregnated with fastidious organisms at -80 °C without the addition of cryoprotectants. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2020.105969

    authors： Villa L,Torreblanca A,Otero L,Carreño F,Campo R,López-Escobar M,Vazquez F

    更新日期：2020-08-01 00:00:00

  • A new method for the preservation of axenic fungal cultures.

    abstract：:Microbiological work requires a reliable source of cultures that are not only well defined and taxonomically determined, but are also adequately preserved without changes in their morphological, physiological and genetic traits. Here we describe an easy, cost effective and rapid method for reliably preserving filament...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.02.009

    authors： Hu X,Webster G,Xie L,Yu C,Li Y,Liao X

    更新日期：2014-04-01 00:00:00

  • Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers.

    abstract：:A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.09.002

    authors： Santos CS,Ramos JN,Vieira VV,Pinheiro CS,Meyer R,Alcantara-Neves NM,Ramos RT,Silva A,Hirata R Jr,Felicori L,de Alegría Puig CR,Navas J,Azevedo V,Mattos-Guaraldi AL,Pacheco LGC

    更新日期：2017-11-01 00:00:00

  • Detection and typing of Helicobacter pylori cagA/vacA genes by radioactive, one-step polymerase chain reaction in stool samples from children.

    abstract：:The detection and molecular typing of Helicobacter pylori virulence genes in human stool specimens by polymerase chain reaction (PCR) require an adequate amount of bacterial DNA and an appropriately adjusted PCR protocol. DNA was isolated from stool samples of 39 H. pylori-infected and nine uninfected Colombian childr...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(02)00158-6

    authors： Sicinschi LA,Correa P,Bravo LE,Schneider BG

    更新日期：2003-02-01 00:00:00

  • Adhesion of Yersinia enterocolitica to non-cultured epithelial cells from pig and rabbit ilea.

    abstract：:A simple and reliable method was developed to isolate intact epithelial cells from pig and rabbit ilea and these were used to investigate the adhesion of Yersinia enterocolitica. Hydrophobic interaction was eliminated by treating the bacterial culture with 0.8 M tetramethyl urea (TMU). Virulent strains of Y. enterocol...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.08.009

    authors： Hussein HM,Fenwick SG,Lumsden JS

    更新日期：2007-02-01 00:00:00

  • Construction and evaluation of pMycoFos, a fosmid shuttle vector for Mycobacterium spp. with inducible gene expression and copy number control.

    abstract：:Molecular tools for Gram-positive bacteria such as Mycobacterium are less well-developed than those for Gram-negatives such as Escherichiacoli. This has slowed the molecular-genetic characterisation of Mycobacterium spp, which is unfortunate, since this genus has high medical, environmental and industrial significance...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2011.06.005

    authors： Ly MA,Liew EF,Le NB,Coleman NV

    更新日期：2011-09-01 00:00:00

  • High density whole genome fingerprinting of methicillin-resistant and -susceptible strains of Staphylococcus aureus in search of phenotype-specific molecular determinants.

    abstract：:AFLP is a selective restriction fragment amplification method generating DNA fingerprints for microbial isolates. We present high-throughput AFLP (htAFLP) to characterize molecular markers associated with bacterial phenotypes. Methicillin-resistant and -susceptible isolates of Staphylococcus aureus have been used for ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.07.013

    authors： Savelkoul PH,Melles DC,Buffing N,Gorkink R,Simons G,van Belkum A

    更新日期：2007-10-01 00:00:00

  • Hybridization monitor: a method for identifying differences between complex genomes.

    abstract：:We have developed a method to identify and amplify differential fragments between two complex genomes. This technique, named hybridization-monitored genome differential analysis (HMDA), incorporates a monitor system into a PCR-based solid subtraction hybridization that tracks the entire hybridization process. This is ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.05.010

    authors： Yueqing C,Zhengbo H,Zhongkang W,Youping Y,Guoxiong P,Yuxian X

    更新日期：2006-03-01 00:00:00

  • Use of green fluorescent protein to quantify the growth of Colletotrichum during infection of tobacco.

    abstract：:To develop a quantitative assay of fungal growth inside plant tissues, strains of Colletotrichum destructivum and Colletotrichum orbiculare were transformed with a modified green fluorescent protein (GFP) gene fused with a glyceraldehyde-3-phosphate dehydrogenase promoter from Aspergillus nidulans. Transformants expre...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(02)00234-8

    authors： Chen N,Hsiang T,Goodwin PH

    更新日期：2003-04-01 00:00:00

  • A culture apparatus for maintaining H2 at sub-nanomolar concentrations.

    abstract：:We devised a microbial culture apparatus capable of maintaining sub-nanomolar H2 concentrations. This apparatus provides a method for study of interspecies hydrogen transfer by externally fulfilling the thermodynamic requirement for low H2 concentrations, thereby obviating the need for use of cocultures to study some ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(99)00125-6

    authors： Valentine DL,Reeburgh WS,Blanton DC

    更新日期：2000-02-01 00:00:00

  • Integration of biological pre-treatment methods for increased energy recovery from paper and pulp biosludge.

    abstract：:The paper and pulp industry (PPI) produces high quantities of solid and liquid discharge and is regarded as the most polluting industry in the world causing adverse effects to environments and human beings. Hence changes in the way PPI sludge and waste materials are treated is urgently required. Nearly, 10 million ton...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.mimet.2019.03.015

    authors： Chakraborty D,Shelvapulle S,Reddy KR,Kulkarni RV,Puttaiahgowda YM,Naveen S,Raghu AV

    更新日期：2019-05-01 00:00:00

  • Analysis of a diverse assemblage of diazotrophic bacteria from Spartina alterniflora using DGGE and clone library screening.

    abstract：:Methods to assess the diversity of the diazotroph assemblage in the rhizosphere of the salt marsh cordgrass, Spartina alterniflora were examined. The effectiveness of nifH PCR-denaturing gradient gel electrophoresis (DGGE) was compared to that of nifH clone library analysis. Seventeen DGGE gel bands were sequenced and...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2008.02.005

    authors： Lovell CR,Decker PV,Bagwell CE,Thompson S,Matsui GY

    更新日期：2008-05-01 00:00:00

  • Codon-improved fluorescent proteins in investigation of Staphylococcus aureus host pathogen interactions.

    abstract：:Here we present the use of three fluorescent proteins in Staphylococcus aureus, Cerulean, PA-GFP, and mRFPmars. All molecules have an improved codon adaptation for expression in the A + T rich organisms and extend the fluorescent protein portfolio in staphylococcal research. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.07.022

    authors： Paprotka K,Giese B,Fraunholz MJ

    更新日期：2010-10-01 00:00:00

  • A genomic library-based amplification approach (GL-PCR) for the mapping of multiple IS6110 insertion sites and strain differentiation of Mycobacterium tuberculosis.

    abstract：:Evidence suggests that insertion of the IS6110 element is not without consequence to the biology of Mycobacterium tuberculosis complex strains. Thus, mapping of multiple IS6110 insertion sites in the genome of biomedically relevant clinical isolates would result in a better understanding of the role of this mobile ele...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.03.021

    authors： Namouchi A,Mardassi H

    更新日期：2006-11-01 00:00:00

  • Dual fluorescence system for flow cytometric analysis of Escherichia coli transcriptional response in multi-species context.

    abstract：:When studying interspecies interactions in a bacterial consortium, it may be desirable to analyze one species' transcriptional response as influenced by the other species. We developed a dual fluorescence system of Escherichia coli for Fluorescence-Activated Cell Sorter (FACS)-based analysis for such a purpose. First,...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2008.09.015

    authors： Miao H,Ratnasingam S,Pu CS,Desai MM,Sze CC

    更新日期：2009-02-01 00:00:00

  • Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes.

    abstract：:Two rapid in situ enumeration methods using fluorescent probes were used to assess the physiological activities of Klebsiella pneumoniae biofilms on stainless steel. Fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and rhodamine 123 (Rh 123), were chosen to perform this study. CTC is a soluble redox ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/0167-7012(94)90058-2

    authors： Yu FP,McFeters GA

    更新日期：1994-01-01 00:00:00

  • Optimization of processing conditions for the quantification of enterococci biofilms using microtitre-plates.

    abstract：:We performed a comparison of four selected protocols from the literature using microtitre-plate and colorimetric biomass assay for evaluation of enterococci biofilm formation ability and optimized a protocol after the identification and stringent performance of biofilm formation steps. The optimized protocol uses a dy...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.11.007

    authors： Extremina CI,Costa L,Aguiar AI,Peixe L,Fonseca AP

    更新日期：2011-02-01 00:00:00

  • Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

    abstract：:A rapid and reliable bacterial source tracking (BST) method is essential to counter risks to human health posed by fecal contamination of surface waters. Genetic fingerprinting methods, such as repetitive sequence based-PCR (rep-PCR), have shown promise as BST tools but are time-consuming and labor-intensive. In this ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.10.012

    authors： Siegrist TJ,Anderson PD,Huen WH,Kleinheinz GT,McDermott CM,Sandrin TR

    更新日期：2007-03-01 00:00:00

  • Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization.

    abstract：:Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-p...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.11.005

    authors： Gou H,Li J,Cai R,Song S,Li M,Yang D,Jiang Z,Li Y,Chu P,Li C

    更新日期：2018-01-01 00:00:00

  • Construction of a conditional lethal Salmonella mutant via genetic recombination using the ara system and asd gene.

    abstract：:In order to construct a conditional lethal Salmonella mutant, an arabinose-regulated recombinant genetic system was used. The Salmonella aspartate semialdehyde dehydrogenase (asd) gene was localized under the control of araC P(araBAD) in a plasmid to create the araC P(araBAD)::asd cassette. The cassette was cloned int...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2011.08.004

    authors： Kim SW,Kang HY,Hur J,Gal SW,Bang WY,Cho KK,Kim CW,Bahk JD,Lee JH

    更新日期：2011-11-01 00:00:00

  • Quantifying biofilm structure using image analysis.

    abstract：:We have developed and implemented methods of extracting morphological features from images of biofilms in order to quantify the characteristics of the inherent heterogeneity. This is a first step towards quantifying the relationship between biofilm heterogeneity and the underlying processes, such as mass-transport dyn...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(99)00097-4

    authors： Yang X,Beyenal H,Harkin G,Lewandowski Z

    更新日期：2000-01-01 00:00:00