Abstract:
:AFLP is a selective restriction fragment amplification method generating DNA fingerprints for microbial isolates. We present high-throughput AFLP (htAFLP) to characterize molecular markers associated with bacterial phenotypes. Methicillin-resistant and -susceptible isolates of Staphylococcus aureus have been used for this model study in conjunction with the available S. aureus genome sequences. This facilitates the calculation of theoretical AFLP fingerprints, comparison of these fingerprints with genuine experimental fingerprints, and the subsequent identification of polymorphic AFLP markers without sequence analysis. Analysis of 46 MRSA and 46 MSSA strains by 39 different AFLP reactions generated more than 2500 fragments per strain and an overall number of 6180 scorable markers within all strains. We successfully identify MRSA specific markers and elaborate on the general applicability of the htAFLP approach. This method can be applied to any microbial species for which at least one full-genome sequence is available.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Savelkoul PH,Melles DC,Buffing N,Gorkink R,Simons G,van Belkum Adoi
10.1016/j.mimet.2007.07.013subject
Has Abstractpub_date
2007-10-01 00:00:00pages
44-54issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(07)00257-6journal_volume
71pub_type
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