Microanalysis of antimicrobial properties of human fluids.


:Host defense responses of animals and plants to pathogenic microbes are mediated in part by the release of antimicrobial substances into tissue fluids. Exploration of the antimicrobial properties of tissue fluids is often limited by their small quantity. We have developed assays of antimicrobial activity that require only 1 microl of fluid. Using normal nasal secretions as a model mucosal fluid we demonstrated that the kinetics of the 1 microl colony-forming unit (CFU) assays were equivalent to the larger CFU assays. The handling of viscous mucin-containing fluids was facilitated by pretreatment with N-acetylcysteine (NAC), a treatment that did not alter the performance of the assay. This low-volume assay will facilitate studies of the antimicrobial properties of scarce biological fluids.


J Microbiol Methods


Cole AM,Wu M,Kim YH,Ganz T




Has Abstract


2000-07-01 00:00:00














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    journal_title:Journal of microbiological methods

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    authors: Walberg M,Gaustad P,Steen HB

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    journal_title:Journal of microbiological methods

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  • Optimization of processing conditions for the quantification of enterococci biofilms using microtitre-plates.

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    journal_title:Journal of microbiological methods

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    authors: Extremina CI,Costa L,Aguiar AI,Peixe L,Fonseca AP

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    journal_title:Journal of microbiological methods

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    authors: Morgenegg S,Maibach R,Chaperon DN,Herzog K,Altwegg M

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    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Errampalli D,Leung K,Cassidy MB,Kostrzynska M,Blears M,Lee H,Trevors JT

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    journal_title:Journal of microbiological methods

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    authors: Rivas L,Dykes GA,Fegan N

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    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Yokouchi H,Takeyama H,Miyashita H,Maruyama T,Matsunaga T

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    pub_type: 杂志文章


    authors: Doherty SB,Wang L,Ross RP,Stanton C,Fitzgerald GF,Brodkorb A

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    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Chakraborty D,Shelvapulle S,Reddy KR,Kulkarni RV,Puttaiahgowda YM,Naveen S,Raghu AV

    更新日期:2019-05-01 00:00:00

  • Establishment of a semi-automated pathogen DNA isolation from whole blood and comparison with commercially available kits.

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    pub_type: 杂志文章


    authors: Wiesinger-Mayr H,Jordana-Lluch E,Martró E,Schoenthaler S,Noehammer C

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  • Development of a strain-specific assay for detection of viable Lactobacillus sp. HOFG1 after application to cattle feed.

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    journal_title:Journal of microbiological methods

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Bai X,Liu Z,Ji S,Gottschalk M,Zheng H,Xu J

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    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


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    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Zupan J,Mavri J,Raspor P

    更新日期:2009-12-01 00:00:00