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Evaluation of 5'-nuclease and hybridization probe assays for the detection of shiga toxin-producing Escherichia coli in human stools.

Abstract：

:5'-Nuclease and a hybridization probe assays for the detection of shiga toxin-producing Escherichia coli were validated with regard to selectivity, analytical sensitivity, reproducibility and clinical performance. Both assays were capable of detecting the classical stx(1) and stx(2) genes when challenged with reference strains of E. coli (n=40), although 1 to 4 minority sequence variants, whose clinical relevance is limited (stx(1c), stx(1d), and stx(2f)), were detected less efficiently or not at all by one or both assays. No cross reaction was observed for both assays with 37 strains representing other gastrointestinal pathogens, or normal gastrointestinal flora. Analytical sensitivity ranged from 3.07 to 3.52 log(10) and 3.42 to 4.63 log(10) CFU/g of stool for 5'-nuclease and hybridization probe assay, respectively. Reproducibility was high with coefficients of variation of

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Schuurman T,Roovers A,van der Zwaluw WK,van Zwet AA,Sabbe LJ,Kooistra-Smid AM,van Duynhoven YT

doi

10.1016/j.mimet.2007.05.016

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

406-15

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(07)00184-4

journal_volume

70

pub_type

杂志文章

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