Abstract:
:This study investigated the various commercially available kits and 'in-house' methods to extract DNA from Gram-negative and Gram-positive bacteria, yeast and fungal agents in commonly employed blood culture material. The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template preparation kit, Puregene DNA extraction kit, boiling, glass beads/sonication and wash/alkali/heat lysis. The results indicated that a simple wash/alkali/heat lysis method was the most sensitive, reproducible, simple and cost-effective extraction method. This was the only method which removed any PCR inhibitors and inherent DNA which existed in virgin BacT/Alert aerobic, anaerobic and paediatric blood culture material. Contaminating microbial DNA from Lactococcus lactis or Bacillus coagulans was identified in all batches of BacT/Alert FAN aerobic blood culture material examined.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Millar BC,Jiru X,Moore JE,Earle JAdoi
10.1016/s0167-7012(00)00174-3subject
Has Abstractpub_date
2000-10-01 00:00:00pages
139-47issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(00)00174-3journal_volume
42pub_type
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