Abstract：
:Clostridium difficile is an enteric pathogen that causes approximately 20% to 30% of antibiotic-associated diarrhea. In recent years, there has been a substantial rise in the rate of C. difficile infections as well as the emergence of virulent and antibiotic resistant C. difficile strains. So, there is an urgent need for the identification of therapeutic potential targets and development of new drugs for the treatment and prevention of C. difficile infections. In the current study, we used a hybrid approach by combining sequence similarity-based approach and protein-protein interaction network topology-based approach to identify and characterize the potential drug targets of C. difficile. A total of 155 putative drug targets of C. difficile were identified and the metabolic pathway analysis of these putative drug targets using DAVID revealed that 46 of them are involved in 9 metabolic pathways. In-silico characterization of these proteins identified seven proteins involved in pathogen-specific peptidoglycan biosynthesis pathway. Three promising targets viz. homoserine dehydrogenase, aspartate-semialdehyde dehydrogenase and aspartokinase etc. were found to be involved in multiple enzymatic pathways of the pathogen. These 3 drug targets are of particular interest as they can be used for developing effective drugs against multi-drug resistant C. difficile strain 630 in the near future.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Lohani M,Dhasmana A,Haque S,Wahid M,Jawed A,Dar SA,Mandal RK,Areeshi MY,Khan Sdoi
10.1016/j.mimet.2017.02.008subject
Has Abstractpub_date
2017-05-01 00:00:00pages
6-10eissn
0167-7012issn
1872-8359pii
S0167-7012(17)30048-9journal_volume
136pub_type
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