qPCR assays to quantify genes and gene expression associated with microbial perchlorate reduction.

Abstract:

:Quantitative PCR (qPCR) assays targeting cld (developed in this work) and pcrA (previously described) were used to quantify these perchlorate-related genes in a perchlorate-reducing enrichment culture. Transcript copies were quantified in perchlorate-reducing Rhodocyclaceae strain JDS4. Oxygen and nitrate inhibited expression of cld and pcrA.

journal_name

J Microbiol Methods

authors

De Long SK,Kinney KA,Kirisits MJ

doi

10.1016/j.mimet.2010.09.002

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

270-4

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(10)00306-4

journal_volume

83

pub_type

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