Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements.

Abstract:

:Surface interfacial physiology is particularly important to unicellular organisms with regard to maintenance of optimal cell function. Bacterial cell surfaces possess net negative electrostatic charge by virtue of ionized phosphoryl and carboxylate substituents on outer cell envelope macromolecules which are exposed to the extracellular environment. The degree of peripheral electronegativity influences overall cell surface polarity and can be assessed on the basis of zeta potential which is most often determined by estimating the electrophoretic mobility of cells in an electric field. The purpose of this review is to provide bacteriologists with assistance as they seek to better understand available instrumentation and fundamental principles concerning the estimation of zeta potential as it relates to bacterial surface physiology.

journal_name

J Microbiol Methods

authors

Wilson WW,Wade MM,Holman SC,Champlin FR

doi

10.1016/s0167-7012(00)00224-4

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

153-64

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167701200002244

journal_volume

43

pub_type

杂志文章,评审
  • A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis.

    abstract::We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2018.09.018

    authors: Aono A,Chikamatsu K,Yamada H,Igarashi Y,Murase Y,Takaki A,Mitarai S

    更新日期:2018-11-01 00:00:00

  • A simple binomial test for estimating sequencing errors in public repository 16S rRNA sequences.

    abstract::Sequences in public databases may contain a number of sequencing errors. A double binomial model describing the distribution of indel-excluded similarity coefficients (S) among repeatedly sequenced 16S rRNA was previously developed and it produced a confidence interval of S useful for testing sequence identity among s...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2007.11.013

    authors: Zo YG,Colwell RR

    更新日期:2008-02-01 00:00:00

  • Hybridization of genomic DNA to microarrays: a challenge for the analysis of environmental samples.

    abstract::The use of DNA microarrays for detection and identification of bacteria and genes of interest from various environments (e.g. soil, sediment, water column...) is a major challenge for microbiologists working on functional diversity. So far, most of the genomic methods that have been described rely on the use of taxono...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.mimet.2006.11.007

    authors: Avarre JC,de Lajudie P,Béna G

    更新日期:2007-05-01 00:00:00

  • Influence of PCR cycle number on 16S rRNA gene amplicon sequencing of low biomass samples.

    abstract::The objective of this study was to evaluate the effects of increased PCR cycle number on sequencing results from samples with low microbial biomass, including bovine milk, and murine pelage and blood. We hypothesized that subjecting DNA from such samples to higher PCR cycle numbers would increase 16S rRNA sequencing c...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106033

    authors: Witzke MC,Gullic A,Yang P,Bivens NJ,Adkins PRF,Ericsson AC

    更新日期:2020-09-01 00:00:00

  • Use of both 16S rRNA and engineered functional genes with real-time PCR to quantify an engineered, PCB-degrading Rhodococcus in soil.

    abstract::A real-time PCR (RTm-PCR) assay using fluorescently labeled oligonucleotides (TaqMan probes) was used to detect and quantify the recombinant Rhodococcus sp. strain RHA1(fcb) in soil. One primer and probe set targeted a hypervariable region of the 16S rRNA gene unique to strain RHA1(fcb) and its phylogenetic relatives,...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(02)00067-2

    authors: Rodrigues JL,Aiello MR,Urbance JW,Tsoi TV,Tiedje JM

    更新日期:2002-10-01 00:00:00

  • Long-term monitoring of biofilm growth and disinfection using a quartz crystal microbalance and reflectance measurements.

    abstract::A biofilm reactor was constructed to monitor the long-term growth and removal of biofilms as monitored by the use of a quartz crystal microbalance (QCM) and a novel optical method. The optical method measures the reflectance of white light off the surface of the quartz crystal microbalance electrode (gold) for determi...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2006.01.016

    authors: Reipa V,Almeida J,Cole KD

    更新日期:2006-09-01 00:00:00

  • Improving the selection efficiency of the counter-selection marker pheS* for the genetic engineering of Bacillus amyloliquefaciens.

    abstract::Bacillus subtilis pheS was genetically modified to obtain a counter-selection marker with high selection efficiency in Bacillus amyloliquefaciens. The application of the new replication-thermosensitive integrative vector pNZTM1, containing this marker, pheSBsT255S/A309G, with a two-step replacement recombination proce...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2018.03.011

    authors: Kharchenko MS,Teslya PN,Babaeva MN,Zakataeva NP

    更新日期:2018-05-01 00:00:00

  • Optimization of media components for production of antimicrobial compound by Brevibacillus brevis EGS9 isolated from mangrove ecosystem.

    abstract::The aim of the present study was to show the efficiency of multi-factorial experimental design to investigate factors affecting the antimicrobial compound production by Brevibacillus brevis EGS9 isolated from mangrove sediment soil. The isolate utilizing different carbon and nitrogen sources showed different growth ch...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2017.09.010

    authors: T A,P SK

    更新日期:2017-11-01 00:00:00

  • A comparison of silver ion to streptavidin coated microplates.

    abstract::Direct comparisons are made between covalently linked streptavidin and silver ion coated microplates. Both coatings can immobilize biotinylated molecules. Silver ion coated microplate wells can immobilize 1.8 times higher amounts of biotin labeled horseradish peroxidase. The quantitation range and capacity for the cap...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(00)00248-7

    authors: Bonen MR,García AA,Hoffman SA

    更新日期:2001-03-01 00:00:00

  • A new microarray platform for whole-genome expression profiling of Mycobacterium tuberculosis.

    abstract::Microarrays have allowed gene expression profiling to progress from the gene level to the genome level, and oligonucleotide microarrays have become the platform of choice for large-scale, targeted gene expression studies. cDNA arrays and spotted oligonucleotide arrays have gradually given way to in situ synthesized ol...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2013.12.009

    authors: Venkataraman B,Vasudevan M,Gupta A

    更新日期:2014-02-01 00:00:00

  • The influence of agar brands and micronutrients in the growth optimization of Granulicella sp. (Acidobacteriota).

    abstract::Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2021.106148

    authors: Costa OYA,Kuramae EE

    更新日期:2021-01-20 00:00:00

  • EUCAST recommendations for antimicrobial susceptibility testing applied to the three main Campylobacter species isolated in humans.

    abstract::Antimicrobial susceptibility testing of Campylobacter isolates is of great importance for treatment options especially in systemic diseases. The European Committee for Antimicrobial Susceptibility Testing (EUCAST) recently proposed epidemiological cut-offs (ECOFFs) for a limited number of antimicrobial compounds and f...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2015.10.018

    authors: Sifré E,Salha BA,Ducournau A,Floch P,Chardon H,Mégraud F,Lehours P

    更新日期:2015-12-01 00:00:00

  • Carbohydrates and glycoproteins of Bacillus anthracis and related bacilli: targets for biodetection.

    abstract::The spore is the form released in a bioterrorism attack. There is a real need for definition of new targets for Bacillus anthracis that might be incorporated into emerging biodetection technologies. Particularly of interest are macromolecules found in B. anthracis that are (1) spore-specific, (2) readily accessible on...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/s0167-7012(03)00095-2

    authors: Fox A,Stewart GC,Waller LN,Fox KF,Harley WM,Price RL

    更新日期:2003-08-01 00:00:00

  • Comparative SELDI-TOF-MS profiling of low-molecular-mass proteins from Lignosus rhinocerus (Cooke) Ryvarden grown under stirred and static conditions of liquid fermentation.

    abstract::Mushrooms are considered as important source of biologically active compounds which include low-molecular-mass protein/peptides (LMMP). In this study, we attempted to profile the LMMP from Lignosus rhinocerus, a wild medicinal mushroom, grown by static cultures (SC) and in stirred tank reactor (STR). Crude water extra...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2011.07.005

    authors: Lau BF,Aminudin N,Abdullah N

    更新日期:2011-10-01 00:00:00

  • Revisiting the mechanistic pathways for bacterial mediated synthesis of noble metal nanoparticles.

    abstract::Synthesis and application of reliable nanoscale materials is a progressive domain and the limelight of modern nanotechnology. Conventional physicochemical approaches for the synthesis of metal nanoparticles have become obsolete owing to costly and hazardous materials. There is a need to explore alternative, cost-effec...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.mimet.2019.02.010

    authors: Ali J,Ali N,Wang L,Waseem H,Pan G

    更新日期:2019-04-01 00:00:00

  • 13CO2 pulse labelling of plants in tandem with stable isotope probing: methodological considerations for examining microbial function in the rhizosphere.

    abstract::Recently developed 13CO2 pulse labelling and stable isotope probing (SIP) methods offer the potential to track 13C-labelled plant photosynthate into phylogenetic groups of microbial taxa in the rhizosphere, permitting an examination of the link between soil microbial diversity and carbon flow in situ. We tested the fe...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2004.03.011

    authors: Griffiths RI,Manefield M,Ostle N,McNamara N,O'Donnell AG,Bailey MJ,Whiteley AS

    更新日期:2004-07-01 00:00:00

  • A TaqMan-PCR protocol for quantification and differentiation of the phytopathogenic Clavibacter michiganensis subspecies.

    abstract::Real-time TaqMan-PCR assays were developed for detection, differentiation and absolute quantification of the pathogenic subspecies of Clavibacter michiganensis (Cm) in one single PCR run. The designed primer pair, targeting intergenic sequences of the rRNA operon (ITS) common in all subspecies, was suitable for the am...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(02)00152-5

    authors: Bach HJ,Jessen I,Schloter M,Munch JC

    更新日期:2003-01-01 00:00:00

  • A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica.

    abstract::Development of a high-throughput eukaryotic screening procedure is important to increase success in obtaining improved enzymes through directed enzyme evolution. This procedure was developed for the yeast Yarrowia lipolytica which becomes the second eukaryotic host for this purpose. The extracellular lipase Lip2 was u...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2007.06.008

    authors: Bordes F,Fudalej F,Dossat V,Nicaud JM,Marty A

    更新日期:2007-09-01 00:00:00

  • Interrogation of single nucleotide polymorphisms in gnd provides a novel method for molecular serogrouping of clinically important Shiga toxin producing Escherichia coli (STEC) targeted by regulation in the United States, including the "big six" non-O157

    abstract::Escherichia coli O157:H7 has frequently been associated with foodborne infections and is considered an adulterant in raw non-intact beef in the U.S. Shiga toxin-producing E. coli (STEC) belonging to serogroups O26, O45, O103, O111, O121, and O145 (known as the "big six" non-O157) were estimated to cause >70% of foodbo...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.07.005

    authors: Elder JR,Bugarel M,den Bakker HC,Loneragan GH,Nightingale KK

    更新日期:2016-10-01 00:00:00

  • Use of green fluorescent protein to quantify the growth of Colletotrichum during infection of tobacco.

    abstract::To develop a quantitative assay of fungal growth inside plant tissues, strains of Colletotrichum destructivum and Colletotrichum orbiculare were transformed with a modified green fluorescent protein (GFP) gene fused with a glyceraldehyde-3-phosphate dehydrogenase promoter from Aspergillus nidulans. Transformants expre...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(02)00234-8

    authors: Chen N,Hsiang T,Goodwin PH

    更新日期:2003-04-01 00:00:00

  • Consistent production of chlorine-stressed bacteria from non-chlorinated secondary sewage effluents for use in the U.S. Environmental Protection Agency Alternate Test Procedure protocol.

    abstract::The U.S. Environmental Protection Agency Alternative Test Procedure protocol outlines a method to produce chlorine-stressed bacteria for water quality testing. Achieving consistent results is challenging due effluent variability. We describe a starting point for generating chlorine-stressed samples from secondary effl...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2019.105651

    authors: Hutchison JR,Widder MW,Brooks SM,Brennan LM,Souris L,Divito VT,van der Schalie WH,Ozanich RM

    更新日期:2019-08-01 00:00:00

  • Real-time PCR for quantification of eleven individual Fusarium species in cereals.

    abstract::Contamination of cereals with Fusarium species is one of the major sources of mycotoxins in food and feed. Quantification of biomass of Fusarium species is essential to understand the interactions of individual species in disease development. In this study quantitative real-time PCR assays based on the elongation fact...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.10.016

    authors: Nicolaisen M,Suproniene S,Nielsen LK,Lazzaro I,Spliid NH,Justesen AF

    更新日期:2009-03-01 00:00:00

  • A culture apparatus for maintaining H2 at sub-nanomolar concentrations.

    abstract::We devised a microbial culture apparatus capable of maintaining sub-nanomolar H2 concentrations. This apparatus provides a method for study of interspecies hydrogen transfer by externally fulfilling the thermodynamic requirement for low H2 concentrations, thereby obviating the need for use of cocultures to study some ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(99)00125-6

    authors: Valentine DL,Reeburgh WS,Blanton DC

    更新日期:2000-02-01 00:00:00

  • A new method for the construction of a mutant library with a predictable occurrence rate using Poisson distribution.

    abstract::A yeast transcriptional activator, Gcn4p, induces the expression of genes that are involved in amino acid and purine biosynthetic pathways under amino acid starvation. Gcn4p has an acidic activation domain in the central region and a bZIP domain in the C-terminus that is divided into the DNA-binding motif and dimeriza...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2007.02.010

    authors: Seong KM,Park H,Kim SJ,Ha HN,Lee JY,Kim J

    更新日期:2007-06-01 00:00:00

  • Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection.

    abstract::In this study, a simple and rapid method was developed to transform protein A producing Staphylococcus aureus cells into red color and red fluorescent nanobioparticles, which were homogeneous, dispersive and relatively stable with a uniform size of 800 nm. The method consists of reaction with a monotetrazolium redox d...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2015.04.018

    authors: Hu W,Zhang Y,Yang H,Yu J,Wei H

    更新日期:2015-08-01 00:00:00

  • Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS.

    abstract::A protein database was constructed and validated with identification rate over 90% for the 4 most common Gram-negative bacteria on agar plates. By protein masses comparison, 120 bacteria of the 4 species from blood culture bottles were identified. The concordance was high (Kappa=0.906) between our method and conventio...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2014.07.021

    authors: Xiao D,Yang Y,Jiang W,Zhang H,Liu H,Yu H,Xie C,Zhong M,Chen L,Huang W

    更新日期:2014-10-01 00:00:00

  • Development and validation of a droplet digital PCR assay for the detection and quantification of Bartonella species within human clinical samples.

    abstract::This report describes the development, optimization, and validation of a ddPCR assay for the detection of Bartonella spp. DNA within several sample matrices, including clinical blood samples from patients with or without documented Bartonella spp. bacteremia. The Bartonella spp. ddPCR assay was developed based upon pr...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106022

    authors: Maggi RG,Richardson T,Breitschwerdt EB,Miller JC

    更新日期:2020-09-01 00:00:00

  • A highly sensitive and multiplexed method for focused transcript analysis.

    abstract::We describe a novel, multiplexed method for focused transcript analysis of tens to hundreds of genes. In this method TRAC (transcript analysis with aid of affinity capture) mRNA targets, a set of amplifiable detection probes of distinct sizes and biotinylated oligo(dT) capture probe are hybridized in solution. The for...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2006.03.013

    authors: Kataja K,Satokari RM,Arvas M,Takkinen K,Söderlund H

    更新日期:2006-10-01 00:00:00

  • Development of a novel in vitro onychomycosis model for the evaluation of topical antifungal activity.

    abstract::A novel in vitro onychomycosis model was developed to easily predict the topical activity potential of novel antifungal drugs. The model encompasses drug activity and diffusion through bovine hoof slices in a single experimental set-up. Results correspond well with the antifungal susceptibility assay and Franz cell di...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2015.03.009

    authors: Sleven R,Lanckacker E,Boulet G,Delputte P,Maes L,Cos P

    更新日期:2015-05-01 00:00:00

  • Use of the 16S--23S ribosomal genes spacer region in studies of prokaryotic diversity.

    abstract::The description of microbial diversity by molecular culture-independent techniques most often involves the amplification of the 16S rRNA by PCR gene and either analysis of the diversity of amplified molecules (community fingerprinting) that allows the simultaneous study of many samples or the cloning and sequencing of...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(99)00011-1

    authors: García-Martínez J,Acinas SG,Antón AI,Rodríguez-Valera F

    更新日期:1999-05-01 00:00:00