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Development of a strain-specific assay for detection of viable Lactobacillus sp. HOFG1 after application to cattle feed.

Abstract：

:A strain-specific assay was developed for the detection of viable Lactobacillus on cattle feed. The DNA sequences of the 16S rRNA gene and four different 16S/23S rRNA intergenic spacer regions (ISR) from Lactobacillus sp. HOFG1 were determined. Based on these sequences, a strain-specific primer was designed for the amplification of one of the ISRs. When combined with a Lactobacillus group primer, the polymerase chain reaction (PCR) assay detected only Lactobacillus sp. HOFG1 and not other closely related L. animalis or L. murinus strains. The feed assay uses a combination of enrichment culturing and PCR to detect and enumerate viable Lactobacillus sp. HOFG1 after its application onto cattle feed. The high degree of primer specificity and use of selective culturing allows for the detection of viable Lactobacillus which is useful in tracking bacteria applied to complex feed mixtures that contain a high background of endogenous bacteria.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Flint JF,Angert ER

doi

10.1016/j.mimet.2004.12.002

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

235-43

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(04)00357-4

journal_volume

61

pub_type

杂志文章

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