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An integrated culture and real-time PCR method to assess viability of disinfectant treated Bacillus spores using robotics and the MPN quantification method.

Abstract：

:Using robotics and the MPN technique, a 96-microwell method was developed to compare two procedures for enumeration of viable chlorine-treated B. atrophaeus spores: broth-culture enrichment followed by real-time polymerase chain reaction analysis; and filter plating on agar. Recoveries of chlorine-treated spores were improved by broth enrichment over filter plating, whereas recoveries of non-treated spores were not different in the two procedures.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Varughese EA,Wymer LJ,Haugland RA

doi

10.1016/j.mimet.2007.07.011

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

66-70

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(07)00261-8

journal_volume

71

pub_type

杂志文章

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