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Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277.

Abstract：

:To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/mug of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Ravindran CR,Suguna S,Shanmugasundaram S

doi

10.1016/j.mimet.2005.11.011

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

174-6

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(05)00365-9

journal_volume

66

pub_type

杂志文章

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