Abstract：
:Small-colony variants (SCVs) are slow-growing subpopulations of various auxotrophic bacterial strains. Thymidine-dependent SCVs (TD-SCVs) are unable to synthesize thymidine; hence, these variants fail to grow in a medium without thymidine. In this study, we used 10 TD-SCVs of Staphylococcus aureus, of which four strains possessed mecA. We compared the efficacy of a newly modified medium containing thymidine for the detection of TD-SCVs of methicillin-resistant S. aureus (MRSA) to the efficacy of routinely used laboratory media. We observed that none of the 10 TD-SCVs of S. aureus grew in Mueller-Hinton agar, and four TD-SCVs of MRSA failed to grow on all MRSA screening media, except for the ChromID™ MRSA medium. Laboratory tests conducted using medium with thymidine incorporated showed that thymidine did not affect the minimum inhibitory concentrations of oxacillin and cefoxitin for clinical isolates of S. aureus, and was able to detect MRSA, including TD-SCVs. These findings showed that thymidine-incorporated media are able to detect TD-SCVs of MRSA without altering the properties of other clinically isolated MRSA strains.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Horiuchi K,Matsumoto T,Ota Y,Kasuga E,Negishi T,Yaguchi T,Sugano M,Honda Tdoi
10.1016/j.mimet.2015.01.007subject
Has Abstractpub_date
2015-03-01 00:00:00pages
40-4eissn
0167-7012issn
1872-8359pii
S0167-7012(15)00016-0journal_volume
110pub_type
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