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SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome.

Abstract：

:We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Benson RW,Cafarelli TM,Godoy VG

doi

10.1016/j.mimet.2010.12.020

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

479-81

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(10)00451-3

journal_volume

84

pub_type

杂志文章

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