Use of an alternative Archaea-specific probe for methanogen detection.

Abstract:

:An alternative 16S rRNA-targeted oligonucleotide probe specific for Archaea was developed and used for detection of methanogens in anaerobic reactors. The designed probe was checked for its specificity by computer-aided comparative sequence analysis. For in situ application, optimal stringency conditions were adjusted by performing whole cell hybridization using target and nontarget organisms. Anaerobic sludge samples were examined by in situ hybridization for methanogenic populations. The relative abundance of methanogens was monitored with epifluorescence microscopy. Individual cells could be visualized with strong fluorescence signals after hybridization with the newly developed probe.

journal_name

J Microbiol Methods

authors

Jupraputtasri W,Boonapatcharoen N,Cheevadhanarak S,Chaiprasert P,Tanticharoen M,Techkarnjanaruk S

doi

10.1016/j.mimet.2004.11.017

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

95-104

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(04)00329-X

journal_volume

61

pub_type

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