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A panel of bioluminescent whole-cell bacterial biosensors for the screening for new antibacterial substances from natural extracts.

Abstract：

:Whole-cell bacterial biosensors can be applied for the screening of antibacterial properties of extracts. We constructed a biosensor panel consisting of four different bacterial biosensor strains: Escherichia, Staphylococcus, Acinetobacter and Pseudomonas for expanded screening potential. The functionality of the panel was first evaluated with known antibacterial compounds: ethanol, naphthoquinones (juglone, lawsone, plumbagin) and a flavonoid (quercetin). Natural extracts comprise a vast source of potential new antibacterials for diverse functional purposes. To demonstrate the utilization of the panel for screening of a demanding sample material, round-leaved sundew (Drosera rotundifolia) extracts were used as an example. Differences between field- and laboratory originating sundew extracts could be detected. This demonstrates the efficiency of the developed biosensor panel in the rapid screening of the antibacterial properties of plant extracts.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Poikulainen E,Tienaho J,Sarjala T,Santala V

doi

10.1016/j.mimet.2020.106083

subject

Has Abstract

pub_date

2020-10-14 00:00:00

pages

106083

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(20)30799-5

journal_volume

178

pub_type

杂志文章

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