Abstract：
:In this study, a simple and rapid method was developed to transform protein A producing Staphylococcus aureus cells into red color and red fluorescent nanobioparticles, which were homogeneous, dispersive and relatively stable with a uniform size of 800 nm. The method consists of reaction with a monotetrazolium redox dye at 25°C for 15 min and heat inactivation at 65°C for 30 min. This method provided the first S. aureus nanobioparticles with the dual property of red color and red fluorescence. Attributed to the IgG binding site known as protein A on their surface, the nanobioparticles could be used as vectors for immunoassays of many bacteria and viruses. Coagglutination test of Escherichia coli O157:H7 observed by naked eyes showed that the detection limitation of the nanobioprobes was 1∗10(6) CFU/ml, which was about 100 times more sensitive than the natural uncolored S. aureus bioprobes. Red fluorescence detection and analysis of the coagglutination product by a microplate reader lowered the detection limit to 2.5∗10(4) CFU/ml.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Hu W,Zhang Y,Yang H,Yu J,Wei Hdoi
10.1016/j.mimet.2015.04.018subject
Has Abstractpub_date
2015-08-01 00:00:00pages
47-53eissn
0167-7012issn
1872-8359pii
S0167-7012(15)00159-1journal_volume
115pub_type
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