Abstract:
:We performed a comparison of four selected protocols from the literature using microtitre-plate and colorimetric biomass assay for evaluation of enterococci biofilm formation ability and optimized a protocol after the identification and stringent performance of biofilm formation steps. The optimized protocol uses a dynamic model that provided a greater discrimination of enterococci biofilm formation ability, and could better simulate in vivo real conditions. Moreover different biofilm quantification approaches, such as the colorimetric biomass (crystal violet), the resazurin and CFU's assays could be used with the optimized protocol, with adequate reproducibility. This study also recognizes that parameters such as the biofilm formation index (BFi), the cut-off values and the Z' factor provide greater accuracy, possibility of inter and intra laboratory comparison and quality evaluation of the biofilm screening assays, respectively.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Extremina CI,Costa L,Aguiar AI,Peixe L,Fonseca APdoi
10.1016/j.mimet.2010.11.007subject
Has Abstractpub_date
2011-02-01 00:00:00pages
167-73issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(10)00386-6journal_volume
84pub_type
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