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Arsenic interception by cell wall of bacteria observed with surface-enhanced Raman scattering.

Abstract：

:The purpose of this study was to determine the interactions between arsenic (As) resistant bacteria and As, using surface-enhanced Raman scattering (SERS) and Fourier transform infrared (FTIR) spectroscopy. According to our 16S rDNA results, eight bacteria isolated from the environment can be identified to four genera (Arthrobacter, Pseudomonas, Sphingomonas, and Acinetobacter). The bacteria were separated into cell wall and protoplast in the study to assess the As(V) attack. The As(V) stress on bacteria could be identified with SERS, but not with FTIR. The bacteria in our study primarily resist As(V) through sequestration of As(V) by the cell wall. The change in SERS peaks and their relationships with cell wall suggested that As(V) mainly interacts with functional groups on the cell wall including polysaccharides and flavin derivates.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Tian H,Zhuang G,Ma A,Jing C

doi

10.1016/j.mimet.2012.03.018

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

153-8

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(12)00119-4

journal_volume

89

pub_type

杂志文章

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