Abstract：
:Cell wall glycopolymers (CWG) represent an important component of the Gram-positive cell envelope with many biological functions. The mycelial soil bacterium Streptomyces coelicolor A3(2) incorporates two distinct CWGs, polydiglycosylphosphate (PDP) and teichulosonic acid, into the cell wall of its vegetative mycelium but only little is known about their role in the complex life cycle of this microorganism. In this study we established assays to measure the total amount of CWGs in mycelial cell walls and spore walls, to quantify the individual CWGs and to determine the length of PDP. By applying these assays, we discovered that the relative amount of CWGs, especially of PDP, is reduced in spores compared to vegetative mycelium. Furthermore we found that PDP extracted from mycelial cell walls consisted of at least 19 repeating units, whereas spore walls contained substantially longer PDP polymers.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Sigle S,Steblau N,Wohlleben W,Muth Gdoi
10.1016/j.mimet.2016.07.002subject
Has Abstractpub_date
2016-09-01 00:00:00pages
52-57eissn
0167-7012issn
1872-8359pii
S0167-7012(16)30170-1journal_volume
128pub_type
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