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A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay.

Abstract：

:Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P=0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Elwin K,Robinson G,Hadfield SJ,Fairclough HV,Iturriza-Gómara M,Chalmers RM

doi

10.1016/j.mimet.2012.02.006

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

38-40

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(12)00060-7

journal_volume

89

pub_type

杂志文章

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