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A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential.

Abstract：

:We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Gentry DR,Wilding I,Johnson JM,Chen D,Remlinger K,Richards C,Neill S,Zalacain M,Rittenhouse SF,Gwynn MN

doi

10.1016/j.mimet.2010.08.012

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

254-6

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(10)00278-2

journal_volume

83

pub_type

杂志文章

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