Abstract:
:A rapid bead assay for detecting pathogenic bacteria with a simple microfluidic chip-based system was developed. Five oligonucleotide probes corresponding to the 16S rRNA of the targeted bacteria were coupled covalently to fluorescent beads. Four species of bacteria (Escherichia coli, Salmonella enterica subsp. enterica serovar Enteritidis, Yersinia enterocolitica, and Bacillus cereus) were used as representative food-borne pathogenic bacteria. The RNAs extracted from pure cultures of these microorganisms were fluorescently labeled and hybridized to the oligonucleotide probes-immobilized fluorescent beads (Bead assay). The duplexes of RNAs and the probes-immobilized beads were analyzed with the commercially available microfluidic chip-based system. This bead assay provided results within 3 h following RNA extraction from bacterial cells.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Ikeda M,Yamaguchi N,Tani K,Nasu Mdoi
10.1016/j.mimet.2006.03.014subject
Has Abstractpub_date
2006-11-01 00:00:00pages
241-7issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(06)00086-8journal_volume
67pub_type
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