当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > The first performance report for the Bio-Rad Dx CT/NG/MG assay for simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in urogenital samples.

The first performance report for the Bio-Rad Dx CT/NG/MG assay for simultaneous detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasma genitalium in urogenital samples.

Abstract：

:We evaluated the clinical performance of the Bio-Rad Dx CT/NG/MG assay for the detection of Chlamydia trachomatis, Mycoplasma genitalium and Neisseria gonorrhoeae in urogenital samples in comparison with the Roche COBAS® TaqMan® CT assay for C. trachomatis and an in-house TaqMan PCR assay for M. genitalium. Swab specimens were cultured for N. gonorrhoeae. In this prospective study, urogenital samples were obtained from symptomatic and asymptomatic patients attending the sexually transmitted disease clinic of Bordeaux, France, from January to April 2010. A total of 658 clinical specimens (259 male and 180 female urines, 191 vaginal, 21 endocervical and 7 urethral swabs) from 453 patients were analyzed. The prevalence of C. trachomatis and M. genitalium infections was 8.1% (21/260) and 1.9% (5/260) in men and 10.4% (20/193) and 2.1% (4/193) in women, respectively. The Bio-Rad Dx CT/NG/MG clinical sensitivity was 100% for C. trachomatis and M. genitalium in men and women. In male urine, the clinical specificity was 99.6% for C. trachomatis and 100% for M. genitalium. In women, the specificity was 99.5% for swabs and 100% for urines for detecting C. trachomatis and M. genitalium. All seven N. gonorrhoeae PCR-positive samples were also positive by culture. Patients were co-infected in 5/57 cases (8.8%), with C. trachomatis and M. genitalium in three cases, and C. trachomatis and N. gonorrhoeae in two cases. In conclusion, the Bio-Rad Dx CT/NG/MG assay can be recommended for the simultaneous detection of C. trachomatis, M. genitalium and N. gonorrhoeae in urogenital specimens of symptomatic and asymptomatic individuals.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Le Roy C,Le Hen I,Clerc M,Arfel V,Normandin F,Bébéar C,de Barbeyrac B

doi

10.1016/j.mimet.2012.03.009

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

193-7

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(12)00108-X

journal_volume

89

pub_type

杂志文章

相关文献

JOURNAL OF MICROBIOLOGICAL METHODS文献大全
  • Strain discrimination among B. anthracis and related organisms by characterization of bclA polymorphisms using PCR coupled with agarose gel or microchannel fluidics electrophoresis.

    abstract：:The bclA gene codes for the protein backbone of the exosporium glycoprotein BclA of B. anthracis. BclA has a central collagen-like region formed by polymorphic GXX repeats and conserved amino- and carboxy-termini. It is noted here that the bclA gene is also present in the genome of Bacillus cereus and Bacillus thuring...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.04.032

    authors： Castanha ER,Swiger RR,Senior B,Fox A,Waller LN,Fox KF

    更新日期：2006-01-01 00:00:00

  • Status of methods for assessing bacterial cell surface charge properties based on zeta potential measurements.

    abstract：:Surface interfacial physiology is particularly important to unicellular organisms with regard to maintenance of optimal cell function. Bacterial cell surfaces possess net negative electrostatic charge by virtue of ionized phosphoryl and carboxylate substituents on outer cell envelope macromolecules which are exposed t...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章,评审

    doi：10.1016/s0167-7012(00)00224-4

    authors： Wilson WW,Wade MM,Holman SC,Champlin FR

    更新日期：2001-01-01 00:00:00

  • Polymerase chain reaction fingerprints of methicillin-resistant Staphylococcus aureus clinical isolates in Greece are related to certain antibiotypes.

    abstract：:Characterization of clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and representatives of three MRSA clones from other hospitals was performed by arbitrarily primed polymerase chain reaction (AP-PCR) and antibiotic susceptibility patterns. All isolates were mecA-positive and two main antibioty...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(02)00249-x

    authors： Bartzavali-Louki C,Dimitracopoulos G,Spiliopoulou I

    更新日期：2003-06-01 00:00:00

  • Optimization of a Nile Red method for rapid lipid determination in autotrophic, marine microalgae is species dependent.

    abstract：:Several studies have been conducted to develop rapid methods for quantification of lipid content in microalgae, as an alternative for time consuming gravimetric methods. Different studies showed that lipid staining with Nile Red in whole cell suspensions and subsequently quantification by the use of a spectrofluoromet...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.09.009

    authors： Balduyck L,Veryser C,Goiris K,Bruneel C,Muylaert K,Foubert I

    更新日期：2015-11-01 00:00:00

  • Estimation of viable Escherichia coli O157 in surface waters using enrichment in conjunction with immunological detection.

    abstract：:The use of a minimal lactose enrichment broth (MLB) in conjunction with immunomagnetic electrochemiluminescence detection (IM-ECL) was evaluated for the estimation of viable Escherichia coli O157 populations in surface water samples. In principle, E. coli O157 populations (C(initial E. coli O157)) can be derived from ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2004.03.017

    authors： Shelton DR,Higgins JA,Van Kessel JA,Pachepsky YA,Belt K,Karns JS

    更新日期：2004-08-01 00:00:00

  • Proteome mining for the identification and in-silico characterization of putative drug targets of multi-drug resistant Clostridium difficile strain 630.

    abstract：:Clostridium difficile is an enteric pathogen that causes approximately 20% to 30% of antibiotic-associated diarrhea. In recent years, there has been a substantial rise in the rate of C. difficile infections as well as the emergence of virulent and antibiotic resistant C. difficile strains. So, there is an urgent need ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.02.008

    authors： Lohani M,Dhasmana A,Haque S,Wahid M,Jawed A,Dar SA,Mandal RK,Areeshi MY,Khan S

    更新日期：2017-05-01 00:00:00

  • Reproducibility and repeatability of six high-throughput 16S rDNA sequencing protocols for microbiota profiling.

    abstract：:Culture-independent molecular techniques and advances in next generation sequencing (NGS) technologies make large-scale epidemiological studies on microbiota feasible. A challenge using NGS is to obtain high reproducibility and repeatability, which is mostly attained through robust amplification. We aimed to assess th...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.03.003

    authors： Raju SC,Lagström S,Ellonen P,de Vos WM,Eriksson JG,Weiderpass E,Rounge TB

    更新日期：2018-04-01 00:00:00

  • Effects of field conditions on fecal microbiota.

    abstract：:Gut microbiota can provide great insight into host health, and studies of the gut microbiota in wildlife are becoming more common. However, the effects of field conditions on gut microbial samples are unknown. This study addresses the following questions: 1) How do environmental factors such as sunlight and insect inf...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.09.017

    authors： Hale VL,Tan CL,Niu K,Yang Y,Cui D,Zhao H,Knight R,Amato KR

    更新日期：2016-11-01 00:00:00

  • Microextraction and Gas Chromatography/Mass Spectrometry for improved analysis of geosmin and other fungal "off" volatiles in grape juice.

    abstract：:Geosmin is a volatile fungal metabolite with an earthy aroma produced in grape products from rotten grapes. The accumulation of geosmin in grapes is caused by the interaction of Botrytis cinerea and Penicillium expansum. Solid Phase Microextraction (SPME) has great utility for collecting volatile compounds in wine. Ho...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.07.013

    authors： Morales-Valle H,Silva LC,Paterson RR,Oliveira JM,Venâncio A,Lima N

    更新日期：2010-10-01 00:00:00

  • Interrogation of single nucleotide polymorphisms in gnd provides a novel method for molecular serogrouping of clinically important Shiga toxin producing Escherichia coli (STEC) targeted by regulation in the United States, including the "big six" non-O157

    abstract：:Escherichia coli O157:H7 has frequently been associated with foodborne infections and is considered an adulterant in raw non-intact beef in the U.S. Shiga toxin-producing E. coli (STEC) belonging to serogroups O26, O45, O103, O111, O121, and O145 (known as the "big six" non-O157) were estimated to cause >70% of foodbo...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.07.005

    authors： Elder JR,Bugarel M,den Bakker HC,Loneragan GH,Nightingale KK

    更新日期：2016-10-01 00:00:00

  • The identification of carbapenemase types in Enterobacteriaceae by using molecular assay and phenotyping confirmation tests.

    abstract：:In this study, we aimed to identify the molecular carbapenemase types of the Enterobacteriaceae isolates and to evaluate the performance of manually prepared and commercially available combination disc methods and the modified Hodge test. One hundred and forty carbapenemase producing isolates and 45 isolates as contro...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.03.010

    authors： Genc O,Aksu E,Gulcan A

    更新日期：2016-06-01 00:00:00

  • Establishment of a semi-automated pathogen DNA isolation from whole blood and comparison with commercially available kits.

    abstract：:Molecular methods for bacterial pathogen identification are gaining increased importance in routine clinical diagnostic laboratories. Achieving reliable results using DNA based technologies is strongly dependent on pre-analytical processes including isolation of target cells and their DNA of high quality and purity. I...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2011.03.003

    authors： Wiesinger-Mayr H,Jordana-Lluch E,Martró E,Schoenthaler S,Noehammer C

    更新日期：2011-06-01 00:00:00

  • An array of multiplex PCR assays for detection of staphylococcal chromosomal cassette mec (SCCmec) types among staphylococcal isolates.

    abstract：:Staphylococcal Cassette Chromosome mec (SCCmec) element plays a key role in development of resistance by carrying different resistance factors. Therefore, routine and rapid diagnostic approach is considered advantageous for the easy detection of SCCmec elements. So, here we have described the use of three sets of mult...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.105733

    authors： Bhowmik D,Das BJ,Pandey P,Chetri S,Chanda DD,Bhattacharjee A

    更新日期：2019-11-01 00:00:00

  • Real-time reverse transcription PCR for the quantification of the mntH expression of Salmonella enterica as a function of growth phase and phagosome-like conditions.

    abstract：:This article presents an experimental design for measuring the mRNA expression in Salmonella enterica of the mntH gene in phagosome-mimicking conditions. The expression of mntH was quantified by real-time reverse transcription PCR for different S. enterica strains of porcine origin under different biological growth co...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.11.003

    authors： Botteldoorn N,Van Coillie E,Grijspeerdt K,Werbrouck H,Haesebrouck F,Donné E,D'Haese E,Heyndrickx M,Pasmans F,Herman L

    更新日期：2006-07-01 00:00:00

  • On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35.

    abstract：:In this study an on-line electrochemical method was developed to examine the one-step growth cycle (OSGC) of the bacteriophage Bam35. The on-line conditions for monitoring the OSGC and the effect of aeration on the duration of the OSGC were defined. The data indicate that binding of phenyldicarbaundecaborane anions to...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.12.023

    authors： Daugelavicius R,Gaidelyte A,Cvirkaite-Krupovic V,Bamford DH

    更新日期：2007-04-01 00:00:00

  • Evaluation of Ag containing hydroxyapatite coatings to the Candida albicans infection.

    abstract：:In this research work, the synthesis of Ag doped hydroxyapatite coatings for dental or orthopedic implants was performed. The main goal was to determine the influence of Ag content on the roughness and antimicrobial performance of the prepared thin films. The films were deposited on Ti6Al4V alloy by means of RF magnet...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.03.016

    authors： Ciuca S,Badea M,Pozna E,Pana I,Kiss A,Floroian L,Semenescu A,Cotrut CM,Moga M,Vladescu A

    更新日期：2016-06-01 00:00:00

  • Evaluation of two automated enzyme-immunoassays for detection of thermophilic campylobacters in faecal samples from cattle and swine.

    abstract：:We evaluated the performance of two enzyme-immunoassays (EIA) for the detection of naturally occurring, thermophilic Campylobacter spp. found in faecal samples from cattle (n = 21 and n = 26) and swine (n = 43) relative to the standard culture method, and also assuming that none of the tests was the definitive standar...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(99)00080-9

    authors： Hoorfar J,Nielsen EM,Stryhn H,Andersen S

    更新日期：1999-10-01 00:00:00

  • GenomeComp: a visualization tool for microbial genome comparison.

    abstract：:We have developed a software tool, GenomeComp, for summarizing, parsing and visualizing the genome sequences comparison results derived from voluminous BLAST textual output. With GenomeComp, the variation between genomes can be easily highlighted, such as repeat regions, insertions, deletions and rearrangements of gen...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(03)00094-0

    authors： Yang J,Wang J,Yao ZJ,Jin Q,Shen Y,Chen R

    更新日期：2003-09-01 00:00:00

  • A novel screening method based on menadione mediated rapid reduction of tetrazolium salt for testing of anti-mycobacterial agents.

    abstract：:A microplate-based rapid, inexpensive and robust technique is developed by using tetrazolium salt 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and menadione to determine the viability of Mycobacterium tuberculosis, Mycobacterium bovis BCG and Mycobacterium smegmatis bacilli in microp...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.11.013

    authors： Singh U,Akhtar S,Mishra A,Sarkar D

    更新日期：2011-02-01 00:00:00

  • In vitro cell culture methods for investigating Campylobacter invasion mechanisms.

    abstract：:Studying the mechanisms of Campylobacter pathogenesis is complicated by the lack of simple animal models that mimic the disease seen in humans. In vitro cell culture methods provide a useful alternative to investigate the interactions between Campylobacter and the host epithelium that occur during infection. In the ge...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.mimet.2004.12.003

    authors： Friis LM,Pin C,Pearson BM,Wells JM

    更新日期：2005-05-01 00:00:00

  • Relating growth dynamics and glucoamylase excretion of individual Saccharomyces cerevisiae cells.

    abstract：:We have developed a novel flow cytometric procedure that allows determinations of properties of protein excretion in the growth medium on a cell-by-cell basis in Saccharomyces cerevisiae. The procedure is based on labelling of a periplasmically secreted protein with antibodies conjugated to a fluorescent marker such a...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(00)00171-8

    authors： Porro D,Venturini M,Brambilla L,Alberghina L,Vanoni M

    更新日期：2000-09-01 00:00:00

  • A robust and fast bacteria counting method using CdSe/ZnS core/shell quantum dots as labels.

    abstract：:A rapid, sensitive fluorescence measurement method for detecting the bacterial count using CdSe/ZnS as a fluorescence marker was described. High-quality CdSe/ZnS nanocrystals were synthesized and successfully conjugated with bacteria. The fluorescence intensity was proportional to bacterial count in the range of 10(2)...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.09.019

    authors： Fu X,Huang K,Liu S

    更新日期：2009-12-01 00:00:00

  • RNA-conjugated template-switching RT-PCR method for generating an Escherichia coli cDNA library for small RNAs.

    abstract：:The cDNA conversion of RNA molecules is a prerequisite for their analysis. In the case of prokaryotic RNAs, cDNA conversion is difficult due to a lack of the long poly(A) tails that are found in eukaryotic mRNAs. The full cDNAs for eukaryotic mRNAs can be amplified by the reverse transcription polymerase chain reactio...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.05.011

    authors： Ko JH,Lee Y

    更新日期：2006-03-01 00:00:00

  • A tetracycline-inducible expression vector for Streptococcus agalactiae allowing controllable gene expression.

    abstract：:Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.10.020

    authors： Lartigue MF,Bouloc P

    更新日期：2014-01-01 00:00:00

  • A selective medium for the isolation of Corynebacterium species in oral cavities.

    abstract：:Corynebacterium matruchotii is a microbial inhabitant in the oral cavity of humans and is associated with the formation of dental calculi. C. matruchotii forms highly specific morphological units, which are referred to as corn-cobs. Although other Corynebacterium species have frequently been isolated from the oral cav...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.06.005

    authors： Tsuzukibashi O,Uchibori S,Shinozaki-Kuwahara N,Kobayashi T,Takada K,Hirasawa M

    更新日期：2014-09-01 00:00:00

  • A novel method for the measurement of dissolved adenosine and guanosine triphosphate in aquatic habitats: applications to marine microbial ecology.

    abstract：:A novel method for the measurement of dissolved adenosine-5'-triphosphate and guanosine-5'-triphosphate (D-ATP and D-GTP, respectively) in marine and freshwater habitats was developed and applied to samples collected from the oligotrophic North Pacific Ocean. Both D-ATP and D-GTP are co-precipitated by authigenically ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(01)00301-3

    authors： Björkman KM,Karl DM

    更新日期：2001-11-01 00:00:00

  • Development and characterization of a 3D oral mucosa model as a tool for host-pathogen interactions.

    abstract：:The aim of this study was to (i) design, develop and validate a practical and physiologically relevant reconstituted in vitro oral mucosa tissue model and (ii) to assess its applicability in in vitro host-pathogen interactions with C. albicans and S. aureus. Co-culture organotypic constructions were created by incorpo...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.07.004

    authors： de Carvalho Dias K,de Sousa DL,Barbugli PA,Cerri PS,Salih VM,Vergani CE

    更新日期：2018-09-01 00:00:00

  • Influence of PCR cycle number on 16S rRNA gene amplicon sequencing of low biomass samples.

    abstract：:The objective of this study was to evaluate the effects of increased PCR cycle number on sequencing results from samples with low microbial biomass, including bovine milk, and murine pelage and blood. We hypothesized that subjecting DNA from such samples to higher PCR cycle numbers would increase 16S rRNA sequencing c...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2020.106033

    authors： Witzke MC,Gullic A,Yang P,Bivens NJ,Adkins PRF,Ericsson AC

    更新日期：2020-09-01 00:00:00

  • Screening for peptides binding on Phytophthora capsici extracts by phage display.

    abstract：:Phytophthora blight of pepper is a completely destructive plant disease caused by the oomycete pathogen Phytophthora capsici Leonian. Phytophthora disease is responsible for major losses in pepper production and the pathogen can survive in soil in the absence of the host plant for many years. Currently, there are no e...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.04.006

    authors： Park HY,Park HC,Yoon MY

    更新日期：2009-07-01 00:00:00

  • Comparison of culture and biochemical tests with PCR for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli.

    abstract：:Traditional culture and biochemical tests (CBT) were compared with PCR for sensitivity and detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in seeded faeces and clinical samples from diarrhoeic pigs. A duplex PCR system was developed based on primers detecting the tlyA-gene of B. hyodysenteriae and t...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.12.008

    authors： Råsbäck T,Fellström C,Gunnarsson A,Aspán A

    更新日期：2006-08-01 00:00:00