Abstract:
:The purpose of this study was to develop a biosensor based on surface plasmon resonance (SPR) for the rapid identification of C. jejuni in broiler samples. We examined the specificity and sensitivity of commercial antibodies against C. jejuni with six Campylobacter strains and six non-Campylobacter bacterial strains. Antigen-antibody interactions were studied using enzyme-linked immunosorbent assay (ELISA) and a commercially available SPR biosensor platform (Spreeta). Campylobacter cells killed with 0.5% formalin had significant lower antibody reactivity when compared to live cells, or cells inactivated with 0.5% thimerosal or heat (70 degrees C for 3 min) using ELISA. The SPR biosensor showed a good sensitivity with commercial antibodies against C. jejuni at 10(3) CFU/ml and a low cross reactivity with Salmonella serotype typhimurium. The sensitivity of the SPR was similar when testing spiked broiler meat samples. However, research is still needed to reduce the high background observed when sampling meat products.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Wei D,Oyarzabal OA,Huang TS,Balasubramanian S,Sista S,Simonian ALdoi
10.1016/j.mimet.2006.12.002subject
Has Abstractpub_date
2007-04-01 00:00:00pages
78-85issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(06)00349-6journal_volume
69pub_type
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