Abstract：
:The gas vesicles of the cyanobacterium Microcystis sp. collapse under pressures ranging from 0.65-1.10 MPa, determined from turbidity changes in a pressure nephelometer. In turgid cells, collapse occurs at a lower range of pressures; the difference is equal to the cell turgor pressure. The turgor pressure decreases, however, as gas vesicles collapse; this decrease is minimised by calculating the turgor pressure in samples with few of their gas vesicles collapsed. Previously, pressure and turbidity were measured in discrete steps, using analogue meters, or continuously, using chart recorders: turgor pressure was calculated from the mean or median collapse pressures. We describe modifications allowing continuous digital recording; the output was modelled with polynomial or sigmoid functions, the latter providing the best fit over the full collapse-pressure curve; turgor pressure could then be calculated for any point on the collapse-pressure curve. The shape of the collapse-pressure curve was affected by the rate of pressure rise; curves were similar to those from step-wise methods when the pressure was raised at approximately 4 kPa s(-1). Under a rapid, almost instantaneous, rise in pressure there was a larger initial decrease in turgor and from the subsequent recovery the hydraulic conductivity of the cell surface could be calculated; the new method gave improved measurements of the cell volumetric elastic modulus. Following collapse of half the gas vesicles, cells recovered their full turgor pressure after 3 h. This suggests turgor homeostasis. These methods are applicable to other bacteria with gas vesicles, including Escherichia coli, if it could be genetically modified to express transgenic gas vesicles.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Holland DP,Walsby AEdoi
10.1016/j.mimet.2009.02.005subject
Has Abstractpub_date
2009-05-01 00:00:00pages
214-24issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(09)00058-Xjournal_volume
77pub_type
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