当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > A reverse-hybridization test for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs.

A reverse-hybridization test for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs.

Abstract：

:The S. PneumoStrip test is a recently developed reverse hybridization strip-based commercial assay that allows for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs, according to their specific gene sequences. The test was validated with reference strains of 92 different pneumococcal serotypes and with a selection of 75 clinical isolates representing 55 serotypes, showing 100% sensitivity and specificity. The test was also applied to 64 pneumococcal invasive isolates (23 different serotypes) consecutively collected between June 2016 and March 2017, with 60 (93.8%) being serotyped. Four isolates belonging to serotypes 13, 29, and 35B (2 isolates), which are not included in the test, did not produce a hybridization signal with serotype specific probes. The identification of most serotypes causing invasive pneumococcal disease together with the simplicity of performance and results interpretation, and the use of routine laboratory equipment make this test very suitable for most clinical and research laboratories.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Marimón JM,Morales M,Gamen S,Manrique A,Ercibengoa M,Cilla G

doi

10.1016/j.mimet.2017.08.010

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

13-16

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(17)30218-X

journal_volume

143

pub_type

杂志文章

相关文献

JOURNAL OF MICROBIOLOGICAL METHODS文献大全
  • A single-pair method to screen Rickettsia-infected and uninfected whitefly Bemisia tabaci populations.

    abstract：:Bacterial endosymbionts such as Rickettsia and Wolbachia play prominent roles in the development and behaviour of their insect hosts, such as whiteflies, aphids, psyllids and mealybugs. Accumulating studies have emphasized the importance of establishing experimental insect populations that are either lacking or bearin...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.105797

    authors： Liu Y,Fan ZY,An X,Shi PQ,Ahmed MZ,Qiu BL

    更新日期：2020-01-01 00:00:00

  • Development of RNA aptamers for detection of Salmonella Enteritidis.

    abstract：:We developed and evaluated RNA aptamers to analyze their potential for use in detecting Salmonella Enteritidis. The selected aptamer was observed to specifically bind to Salmonella Enteritidis without any cross-reactivity to other Salmonella serovars. Thus, this study suggests that aptamers specific to Salmonella Ente...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.01.014

    authors： Hyeon JY,Chon JW,Choi IS,Park C,Kim DE,Seo KH

    更新日期：2012-04-01 00:00:00

  • A new high-throughput AFLP approach for identification of new genetic polymorphism in the genome of the clonal microorganism Mycobacterium tuberculosis.

    abstract：:We have here applied high-throughput amplified fragment length polymorphism (htAFLP) analysis to strains belonging to the five classical species of the Mycobacterium tuberculosis complex. Using 20 strains, three enzyme combinations and eight selective amplification primer pairs, 24 AFLP reactions were performed per st...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2003.09.018

    authors： van den Braak N,Simons G,Gorkink R,Reijans M,Eadie K,Kremers K,van Soolingen D,Savelkoul P,Verbrugh H,van Belkum A

    更新日期：2004-01-01 00:00:00

  • An integrated culture and real-time PCR method to assess viability of disinfectant treated Bacillus spores using robotics and the MPN quantification method.

    abstract：:Using robotics and the MPN technique, a 96-microwell method was developed to compare two procedures for enumeration of viable chlorine-treated B. atrophaeus spores: broth-culture enrichment followed by real-time polymerase chain reaction analysis; and filter plating on agar. Recoveries of chlorine-treated spores were ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.07.011

    authors： Varughese EA,Wymer LJ,Haugland RA

    更新日期：2007-10-01 00:00:00

  • Proposal of protocols using D-glutamine to optimize the 2,3-bis(2-methoxy-4-nitro-5-sulfophenly)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay for indirect estimation of microbial loads in biofilms of medical importance.

    abstract：:Due to technical problems, biofilm biomasses are difficult to be precisely determined. One reliable strategy is based on the colorimetry of formazan compounds derived from tetrazolium salt reduction. XTT presents some desirable properties that make the biofilm measurements easier. However, cells entrapped within the e...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.12.018

    authors： Gobor T,Corol G,Ferreira LE,Rymovicz AU,Rosa RT,Campelo PM,Rosa EA

    更新日期：2011-02-01 00:00:00

  • A TaqMan-PCR protocol for quantification and differentiation of the phytopathogenic Clavibacter michiganensis subspecies.

    abstract：:Real-time TaqMan-PCR assays were developed for detection, differentiation and absolute quantification of the pathogenic subspecies of Clavibacter michiganensis (Cm) in one single PCR run. The designed primer pair, targeting intergenic sequences of the rRNA operon (ITS) common in all subspecies, was suitable for the am...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(02)00152-5

    authors： Bach HJ,Jessen I,Schloter M,Munch JC

    更新日期：2003-01-01 00:00:00

  • Methodological issues in the quantification of subgingival microorganisms using the checkerboard technique.

    abstract：:The reproducibility and reliability of quantitative microbiological assessments using the DNA-DNA hybridization "checkerboard method" (CKB) were assessed. The data originated from 180 chronic periodontitis patients, who were enrolled in a clinical trial and sampled at baseline, and 3 and 12m post-therapy. The samples ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.01.010

    authors： Dahlen G,Preus HR,Baelum V

    更新日期：2015-03-01 00:00:00

  • The use of activated charcoal for the removal of PCR inhibitors from oyster samples.

    abstract：:Activated charcoal is a carbonaceous adsorbent with a high internal porosity, and hence a large internal surface area. Cells of a strain of Escherichia coli O157:H7 seeded into oyster tissue homogenates were completely bound to untreated charcoal after an incubation period of 15 min at room temperature. In contrast, a...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.09.012

    authors： Abolmaaty A,Gu W,Witkowsky R,Levin RE

    更新日期：2007-02-01 00:00:00

  • Population estimates of Thiobacillus thioparus in composting biofilters by PCR analysis.

    abstract：:A direct most-probable-number polymerase chain reaction method (MPN-PCR) was used to monitor populations of Thiobacillus thioparus in compost biofilters used to treat air contaminated with dymethyl disulphide and ammonia. The PCR method quantified this bacterium at numbers ranging from log 2 to log 8 cells per gram of...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.08.001

    authors： Gibson MJ,Trevors JT,Otten L

    更新日期：2006-05-01 00:00:00

  • YTOX: a rapid toxicity test based on the dehydrogenase activity of Saccharomyces cerevisiae for detection of contaminants in water samples.

    abstract：:A simple generic toxicity method (test) is proposed using baker's yeast to mediate the reduction of the colourless triphenyltetrazolium chloride (TTC) to red, 1,3,5-triphenyl formazan, which can be extracted by dimethyl sulfoxide (DMSO), enabling the identification of reducible toxic compounds (e.g. cadmium, fipronil)...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.04.008

    authors： Gomes LH,Duarte KMR,Kamogawa MY,Ferrarezi JA,Andrino FG,Tavares ACL,da Silva APM,Laguna SE,de Lira SP

    更新日期：2019-06-01 00:00:00

  • A single-step transconjugation system for gene deletion in Aggregatibacter actinomycetemcomitans.

    abstract：:Aggregatibacter (A.) actinomycetemcomitans is a periodontopathogenic bacterium causing aggressive periodontitis. Here we describe a single-step transconjugation system as novel and easily applicable protocol for site-specific genetic manipulation of A. actinomycetemcomitans. Deletion of PgaC, which is involved in the ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.04.002

    authors： Stumpp NS,Konze SA,Gerardy-Schahn R,Stiesch M,Buettner FFR

    更新日期：2018-05-01 00:00:00

  • Evaluation of urine for Leishmania infantum DNA detection by real-time quantitative PCR.

    abstract：:The availability of some sorts of biological samples which require noninvasive collection methods has led to an even greater interest in applying molecular biology on visceral leishmaniasis (VL) diagnosis, since these samples increase the safety and comfort of both patients and health professionals. In this context, t...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.10.002

    authors： Pessoa-E-Silva R,Mendonça Trajano-Silva LA,Lopes da Silva MA,da Cunha Gonçalves-de-Albuquerque S,de Goes TC,Silva de Morais RC,Lopes de Melo F,de Paiva-Cavalcanti M

    更新日期：2016-12-01 00:00:00

  • A two-step procedure for automatic and accurate segmentation of volumetric CLSM biofilm images.

    abstract：:This paper presents a robust two-step segmentation procedure for the study of biofilm structure. Without user intervention, the procedure segments volumetric biofilm images generated by a confocal laser scanning microscopy (CLSM). This automated procedure implements an anisotropic diffusion filter as a preprocessing s...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.05.022

    authors： Yerly J,Hu Y,Jones SM,Martinuzzi RJ

    更新日期：2007-09-01 00:00:00

  • Estimation of viable Escherichia coli O157 in surface waters using enrichment in conjunction with immunological detection.

    abstract：:The use of a minimal lactose enrichment broth (MLB) in conjunction with immunomagnetic electrochemiluminescence detection (IM-ECL) was evaluated for the estimation of viable Escherichia coli O157 populations in surface water samples. In principle, E. coli O157 populations (C(initial E. coli O157)) can be derived from ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2004.03.017

    authors： Shelton DR,Higgins JA,Van Kessel JA,Pachepsky YA,Belt K,Karns JS

    更新日期：2004-08-01 00:00:00

  • Comparative SELDI-TOF-MS profiling of low-molecular-mass proteins from Lignosus rhinocerus (Cooke) Ryvarden grown under stirred and static conditions of liquid fermentation.

    abstract：:Mushrooms are considered as important source of biologically active compounds which include low-molecular-mass protein/peptides (LMMP). In this study, we attempted to profile the LMMP from Lignosus rhinocerus, a wild medicinal mushroom, grown by static cultures (SC) and in stirred tank reactor (STR). Crude water extra...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2011.07.005

    authors： Lau BF,Aminudin N,Abdullah N

    更新日期：2011-10-01 00:00:00

  • Effects of field conditions on fecal microbiota.

    abstract：:Gut microbiota can provide great insight into host health, and studies of the gut microbiota in wildlife are becoming more common. However, the effects of field conditions on gut microbial samples are unknown. This study addresses the following questions: 1) How do environmental factors such as sunlight and insect inf...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.09.017

    authors： Hale VL,Tan CL,Niu K,Yang Y,Cui D,Zhao H,Knight R,Amato KR

    更新日期：2016-11-01 00:00:00

  • Robust at-line quantification of poly(3-hydroxyalkanoate) biosynthesis by flow cytometry using a BODIPY 493/503-SYTO 62 double-staining.

    abstract：:Poly(3-hydroxyalkanoates) (PHAs) are bio-based and biodegradable polyesters which have been considered as a promising alternative to petrol-based plastics. Their bacterial production is a dynamic process in which intracellular polymerization and depolymerization are closely linked and depend on the availability of car...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.10.003

    authors： Karmann S,Follonier S,Bassas-Galia M,Panke S,Zinn M

    更新日期：2016-12-01 00:00:00

  • Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-13C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface.

    abstract：:Specific fatty acids from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) recovered from a per 13C-labeled bacteria can be detected in environmental samples and used as measures of bacterial transport in the subsurface. Detection of palmitic acid (16:0) and oleic acid (18:1) at m/z 271 (255+16) and 299 (28...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(00)00245-1

    authors： Lytle CA,Fuller ME,Gan YM,Peacock A,DeFlaun MF,Onstott TC,White DC

    更新日期：2001-04-01 00:00:00

  • Validation of reference genes for quantitative gene expression analysis in Ralstonia pseudosolanacearum CQPS-1 under environment stress.

    abstract：:Quantitative real-time reverse transcriptase PCR (qRT-PCR) has become the method choice for quantification of gene expression changes, however, the accuracy of the method depends on the stability of reference genes. Ralstonia pseudosolanacearum (R. pseudosolanacearum) is an important plant pathogen, infecting >450 pla...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.04.004

    authors： Yang L,Chen J,Liu Y,Zhang S,Li S,Ding W

    更新日期：2018-05-01 00:00:00

  • Subtle changes in host cell density cause a serious error in monitoring of the intracellular growth of Chlamydia trachomatis in a low-oxygen environment: Proposal for a standardized culture method.

    abstract：:We monitored Chlamydia trachomatis growth in HeLa cells cultured with either DMEM or RPMI medium containing 10% FCS under 2% or 21% O2 conditions for 2 days. Bacterial numbers, host cell numbers, and fibrosis-related gene expression in the host cells were estimated by an inclusion forming unit assay, a cell counting a...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.09.007

    authors： Sakai K,Matsuo J,Watanabe T,Okubo T,Nakamura S,Yamaguchi H

    更新日期：2018-10-01 00:00:00

  • Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds.

    abstract：:A real-time quantitative polymerase chain reaction (Q-PCR) assay was developed for detecting and quantifying Escherichia coli in water samples from agricultural watersheds. The assay included optimization of DNA extraction and purification from water samples, and Q-PCR amplification conditions using newly designed spe...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.02.016

    authors： Khan IU,Gannon V,Kent R,Koning W,Lapen DR,Miller J,Neumann N,Phillips R,Robertson W,Topp E,van Bochove E,Edge TA

    更新日期：2007-06-01 00:00:00

  • Modules for C-terminal epitope tagging of Tetrahymena genes.

    abstract：:Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.07.009

    authors： Kataoka K,Schoeberl UE,Mochizuki K

    更新日期：2010-09-01 00:00:00

  • Variation between observed and true Terminal Restriction Fragment length is dependent on true TRF length and purine content.

    abstract：:Terminal Restriction Fragment (TRF) pattern analysis has become a widely used and informative tool for studying microbial communities. Variation between sequence-determined or true TRF length and observed TRF length (TRF drift) has been previously reported and can significantly affect identification of bacterial speci...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(03)00003-4

    authors： Kaplan CW,Kitts CL

    更新日期：2003-07-01 00:00:00

  • A highly sensitive and multiplexed method for focused transcript analysis.

    abstract：:We describe a novel, multiplexed method for focused transcript analysis of tens to hundreds of genes. In this method TRAC (transcript analysis with aid of affinity capture) mRNA targets, a set of amplifiable detection probes of distinct sizes and biotinylated oligo(dT) capture probe are hybridized in solution. The for...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.03.013

    authors： Kataja K,Satokari RM,Arvas M,Takkinen K,Söderlund H

    更新日期：2006-10-01 00:00:00

  • Bacterial identification using a SCIEX 5800 TOF/TOF MALDI research instrument and an external database.

    abstract：:In our current study we were identifying 26 bacterial isolates using a SCIEX 5800 TOF/TOF MALDI instrument and an external database. The results were compared with the results of a Vitek® MS system and in case of discrepancies at the species level 16s rRNA sequencing was performed for further verification. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.105685

    authors： Huber CA,Pflüger V,Reed S,Cottrell K,Sidjabat HE,Ranasinghe A,Zowawi HM,Harris P,Paterson DL

    更新日期：2019-09-01 00:00:00

  • Optimization of media components for production of antimicrobial compound by Brevibacillus brevis EGS9 isolated from mangrove ecosystem.

    abstract：:The aim of the present study was to show the efficiency of multi-factorial experimental design to investigate factors affecting the antimicrobial compound production by Brevibacillus brevis EGS9 isolated from mangrove sediment soil. The isolate utilizing different carbon and nitrogen sources showed different growth ch...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.09.010

    authors： T A,P SK

    更新日期：2017-11-01 00:00:00

  • A new microarray platform for whole-genome expression profiling of Mycobacterium tuberculosis.

    abstract：:Microarrays have allowed gene expression profiling to progress from the gene level to the genome level, and oligonucleotide microarrays have become the platform of choice for large-scale, targeted gene expression studies. cDNA arrays and spotted oligonucleotide arrays have gradually given way to in situ synthesized ol...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.12.009

    authors： Venkataraman B,Vasudevan M,Gupta A

    更新日期：2014-02-01 00:00:00

  • Development of a rapid detection and enumeration method for thermophilic bacilli in milk powders.

    abstract：:Thermophilic strains of Geobacillus, Anoxybacillus and Bacillus that are able to grow at 55 degrees C and above are recognized as commonly occurring contaminants during the production of milk powders. In particular, Anoxybacillus flavithermus strain C and Bacillus licheniformis strain F are often the most prevalent. W...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2004.09.008

    authors： Rueckert A,Ronimus RS,Morgan HW

    更新日期：2005-02-01 00:00:00

  • Analysis of a diverse assemblage of diazotrophic bacteria from Spartina alterniflora using DGGE and clone library screening.

    abstract：:Methods to assess the diversity of the diazotroph assemblage in the rhizosphere of the salt marsh cordgrass, Spartina alterniflora were examined. The effectiveness of nifH PCR-denaturing gradient gel electrophoresis (DGGE) was compared to that of nifH clone library analysis. Seventeen DGGE gel bands were sequenced and...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2008.02.005

    authors： Lovell CR,Decker PV,Bagwell CE,Thompson S,Matsui GY

    更新日期：2008-05-01 00:00:00

  • Rapid antibiotic susceptibility phenotypic characterization of Staphylococcus aureus using automated microscopy of small numbers of cells.

    abstract：:Staphylococcus aureus remains a leading, virulent pathogen capable of expressing complex drug resistance that requires up to 2-4 days for laboratory analysis. In this study, we evaluate the ability of automated microscopy of immobilized live bacterial cells to differentiate susceptible from non-susceptible responses o...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.12.021

    authors： Price CS,Kon SE,Metzger S

    更新日期：2014-03-01 00:00:00