A comparison of silver ion to streptavidin coated microplates.

Abstract:

:Direct comparisons are made between covalently linked streptavidin and silver ion coated microplates. Both coatings can immobilize biotinylated molecules. Silver ion coated microplate wells can immobilize 1.8 times higher amounts of biotin labeled horseradish peroxidase. The quantitation range and capacity for the capture of horseradish peroxidase using biotin labeled horseradish peroxidase are also greater for silver ion coated microplates. Approximately twice as many anti-horseradish peroxidase antibodies can be immobilized per well using silver ion coated microplates. Higher capacities are presumed to be due to the smaller footprint of silver ions as compared to streptavidin. A direct comparison between the two coatings for a beta-galactosidase ELISA showed that while the silver ion coated microplates gave higher readings, the streptavidin coated microplates exhibited smaller well-to-well variation. However, higher well to well variation for the silver microplates is attributed to the high density of anti-beta-galactosidase antibodies on the microplates and the weak binding of clone GAL-13 to beta-galactosidase, rather than the silver coating. These studies suggest silver ion coated microplates are a desirable alternative to streptavidin plates for quantitative immunoassays.

journal_name

J Microbiol Methods

authors

Bonen MR,García AA,Hoffman SA

doi

10.1016/s0167-7012(00)00248-7

subject

Has Abstract

pub_date

2001-03-01 00:00:00

pages

113-20

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167701200002487

journal_volume

44

pub_type

杂志文章
  • Effects of humic substances on fluorometric DNA quantification and DNA hybridization.

    abstract::DNA extracts from sediment and water samples are often contaminated with coextracted humic-like impurities. Estuarine humic substances and vascular plant extract were used to evaluate the effect of the presence of such impurities on DNA hybridization and quantification. The presence of humic substances and vascular pl...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(01)00296-2

    authors: Bachoon DS,Otero E,Hodson RE

    更新日期:2001-10-01 00:00:00

  • Rapid decay of Salmonella flagella antibodies during human gastroenteritis: a follow up study.

    abstract::An indirect enzyme-linked immunosorbent assay (ELISA) based on Salmonella re-polymerized flagella was employed to measure levels of immunoglobulin (Ig) G, IgM and IgA antibodies in sera from 303 Danish patients diagnosed with either Salmonella enteritidis or Salmonella typhimurium. The antibody-levels were assessed at...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2005.02.006

    authors: Dalby T,Strid MA,Beyer NH,Blom J,Mølbak K,Krogfelt KA

    更新日期:2005-08-01 00:00:00

  • Sequence specific primer extension RNA analysis (SeSPERA) for the investigation of substrate utilization of microbial communities.

    abstract::A simple, fast and cost-effective method for the taxon-specific separation of various 16S rRNAs was developed. This primer extension mediated separation of different RNA species was obtained on agarose gels. The method provides an easy way for the investigation of substrate mediated radioisotope incorporation into the...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2009.07.010

    authors: Franchini AG,Nikolausz M,Kästner M

    更新日期:2009-10-01 00:00:00

  • Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system.

    abstract::A rapid bead assay for detecting pathogenic bacteria with a simple microfluidic chip-based system was developed. Five oligonucleotide probes corresponding to the 16S rRNA of the targeted bacteria were coupled covalently to fluorescent beads. Four species of bacteria (Escherichia coli, Salmonella enterica subsp. enteri...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2006.03.014

    authors: Ikeda M,Yamaguchi N,Tani K,Nasu M

    更新日期:2006-11-01 00:00:00

  • Characterization of a single-chain variable fragment specific to Cronobacter spp. from hybridoma based on outer membrane protein A.

    abstract::Monoclonal antibody and polyclonal antibody specific to Cronobacter spp. had been reported in previous studies. However, the preparation of single-chain variable fragment (scFv) was faster and convenient. Hence, the aim of this study was to construct a scFv using outer membrane protein A (OmpA) of C. sakazakii as anti...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.08.004

    authors: Chen Q,Tao T,Bie X,Lu F,Li Y,Lu Z

    更新日期:2016-10-01 00:00:00

  • An improved high throughput sequencing method for studying oomycete communities.

    abstract::Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2015.01.013

    authors: Sapkota R,Nicolaisen M

    更新日期:2015-03-01 00:00:00

  • Applications of the green fluorescent protein as a molecular marker in environmental microorganisms.

    abstract::In this review, we examine numerous applications of the green fluorescent protein (GFP) marker gene in environmental microbiology research. The GFP and its variants are reviewed and applications in plant-microbe interactions, biofilms, biodegradation, bacterial-protozoan interactions, gene transfer, and biosensors are...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/s0167-7012(99)00024-x

    authors: Errampalli D,Leung K,Cassidy MB,Kostrzynska M,Blears M,Lee H,Trevors JT

    更新日期:1999-04-01 00:00:00

  • Serotype discrimination of encapsulated Streptococcus pneumoniae strains by Fourier-transform infrared spectroscopy and chemometrics.

    abstract::Fourier-transform infrared (FTIR) spectroscopy was evaluated as an alternative method for serotyping encapsulated Streptococcus pneumonia strains. Sixty-nine invasive strains from the Microbiology Laboratory of the Medicine Faculty of University of Lisbon were selected for the analysis. The encapsulated strains used i...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2013.02.008

    authors: Vaz M,Meirinhos-Soares L,Sousa CC,Ramirez M,Melo-Cristino J,Lopes JA

    更新日期:2013-05-01 00:00:00

  • A review on various maleic anhydride antimicrobial polymers.

    abstract::The basic requirement of human beings is better health but the serious health effects and numerous infections caused by rapid growth of harmful pathogens resulting in a large number of deaths and is a significant challenge to modern science. Microbes infecting humans can be stopped in two ways: disinfectants and antim...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.mimet.2019.105650

    authors: Nagaraja A,Jalageri MD,Puttaiahgowda YM,Raghava Reddy K,Raghu AV

    更新日期:2019-08-01 00:00:00

  • Modification of the classical Lancefield assay of group A streptococcal killing to reduce inter-donor variation.

    abstract::The lack of a surrogate-of-immunity assay presents a major barrier to Streptococcus pyogenes research. Modification of the Lancefield assay to include an antibody digestion step reduced inter-donor variation and permitted detection of the anti-streptococcal activity of intravenous immunoglobulin and convalescent serum...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.03.015

    authors: Reglinski M,Lynskey NN,Sriskandan S

    更新日期:2016-05-01 00:00:00

  • Specific detection of cytopathogenic Listeria monocytogenes using a two-step method of immunoseparation and cytotoxicity analysis.

    abstract::The development of rapid methods for detection of viable Listeria monocytogenes is crucial to prevent listeriosis and product recalls. While immunomagnetic separation has been used for isolating Listeria spp., lack of specificity and pathogenicity determination render this method unsatisfactory. A two-step method usin...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2004.10.006

    authors: Gray KM,Bhunia AK

    更新日期:2005-02-01 00:00:00

  • New avenues of controlling microbial infections through anti-microbial and anti-biofilm potentials of green mono-and multi-metallic nanoparticles: A review.

    abstract::Nanoparticles synthesized through the green route deserve special mention because this green technology is not only energy-efficient and cost-effective but also amenable to the environment. Various biological resources have been used for the generation of these 'green nanoparticles'. Biological wastes have also been f...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.mimet.2019.105766

    authors: Das P,Karankar VS

    更新日期:2019-12-01 00:00:00

  • An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux AB labelled bacteria as growth medium.

    abstract::An automated modification of the most-probable-number (MPN) technique has been developed for enumeration of phagotrophic protozoa. The method is based on detection of prey depletion in micro titre plates rather than on presence of protozoa. A transconjugant Pseudomonas fluorescens DR54 labelled with a luxAB gene casse...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(99)00086-x

    authors: Ekelund F,Christensen S,Rønn R,Buhl E,Jacobsen CS

    更新日期:1999-11-01 00:00:00

  • A new high-throughput AFLP approach for identification of new genetic polymorphism in the genome of the clonal microorganism Mycobacterium tuberculosis.

    abstract::We have here applied high-throughput amplified fragment length polymorphism (htAFLP) analysis to strains belonging to the five classical species of the Mycobacterium tuberculosis complex. Using 20 strains, three enzyme combinations and eight selective amplification primer pairs, 24 AFLP reactions were performed per st...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2003.09.018

    authors: van den Braak N,Simons G,Gorkink R,Reijans M,Eadie K,Kremers K,van Soolingen D,Savelkoul P,Verbrugh H,van Belkum A

    更新日期:2004-01-01 00:00:00

  • Identification of mutans streptococcal species by the PCR products of the dex genes.

    abstract::A pair of polymerase chain reaction (PCR) primers was designed on the basis of the nucleotide sequence homology of dextranase genes (dex) of Streptococcus mutans, S. sobrinus and S. downei. The primer pair amplified a 530-bp DNA fragment on the dex genes of mutans streptococcal species: S. mutans, S. sobrinus, S. down...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(01)00263-9

    authors: Igarashi T,Ichikawa K,Yamamoto A,Goto N

    更新日期:2001-08-01 00:00:00

  • Rapid quantification of a microbial surfactant by a simple turbidometric method.

    abstract::Quantification of the biosurfactants produced by a variety of microorganisms is a time taking and difficult task due to the lack of rapid, efficient and accurate methods. This work presents a simple turbidometric method for quantification of crude biosurfactants based on their property to become insoluble at low pH va...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2008.09.010

    authors: Mukherjee S,Das P,Sen R

    更新日期:2009-01-01 00:00:00

  • gyrB as a phylogenetic discriminator for members of the Bacillus anthracis-cereus-thuringiensis group.

    abstract::Bacillus anthracis, the causative agent of the human disease anthrax, Bacillus cereus, a food-borne pathogen capable of causing human illness, and Bacillus thuringiensis, a well-characterized insecticidal toxin producer, all cluster together within a very tight clade (B. cereus group) phylogenetically and are indistin...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2003.11.004

    authors: La Duc MT,Satomi M,Agata N,Venkateswaran K

    更新日期:2004-03-01 00:00:00

  • Simplified amplified-fragment length polymorphism method for genotyping Mycobacterium tuberculosis isolates.

    abstract::A simplified amplified-fragment length polymorphism (AFLP) method was developed and applied to genotype 52 Mycobacterium tuberculosis isolates. This method can be carried out using only one restriction enzyme (XhoI), one double strand adapter, and one PCR primer. The amounts of DNA and DNA polymerase, and the concentr...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2009.07.007

    authors: Viader-Salvadó JM,Flores-Gracia J,Vega-Alonso AS,Treviño-Alvarado VM,Molina-Torres CA,Vera-Cabrera L,Guerrero-Olazarán M

    更新日期:2009-09-01 00:00:00

  • A novel 96-well gel-based assay for determining antifungal activity against filamentous fungi.

    abstract::In recent years the global rise in antibiotic resistance and environmental consciousness lead to a renewed fervour to find and develop novel antibiotics, including antifungals. However, the influence of the environment on antifungal activity is often disregarded and many in vitro assays may cause the activity of certa...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2012.09.025

    authors: Troskie AM,Vlok NM,Rautenbach M

    更新日期:2012-12-01 00:00:00

  • Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study.

    abstract::Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2019.105783

    authors: Qadir S,Ellis O,Keizur EM,Stafylis C,Cortado R,Klausner JD,Adolescent Trials Network (ATN) CARES Team.

    更新日期:2020-01-01 00:00:00

  • Fast fluorometric enumeration of E. coli using passive chip.

    abstract::In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were f...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2019.105680

    authors: Kasap EN,Doğan Ü,Çoğun F,Yıldırım E,Boyacı İH,Çetin D,Suludere Z,Tamer U,Ertaş N

    更新日期:2019-09-01 00:00:00

  • Biotransformation of 12-hydroxystearic acid to gamma-decalactone: Comparison of two separation systems.

    abstract::Biotransformation of natural products to the natural flavoring, gamma-decalactone (GDL), has attracted considerable attention. However, improving its yield is challenging due to its high feedback inhibition of yeast cells, which lowers the productivity of the biotransformation process. In this study, we compared two i...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106041

    authors: Rong S,Guan X,Li Q,Guan S,Cai B,Zhang S

    更新日期:2020-09-03 00:00:00

  • Genotypic characterization of Salmonella by multilocus sequence typing, pulsed-field gel electrophoresis and amplified fragment length polymorphism.

    abstract::Molecular typing is an important tool in surveillance and outbreak investigations of human Salmonella infections. In this study, three molecular typing methods were used to investigate the discriminatory ability, reproducibility and the genetic relationship between 110 Salmonella enterica subspecies enterica isolates....

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2005.03.006

    authors: Torpdahl M,Skov MN,Sandvang D,Baggesen DL

    更新日期:2005-11-01 00:00:00

  • A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material.

    abstract::This study investigated the various commercially available kits and 'in-house' methods to extract DNA from Gram-negative and Gram-positive bacteria, yeast and fungal agents in commonly employed blood culture material. The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template prepar...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(00)00174-3

    authors: Millar BC,Jiru X,Moore JE,Earle JA

    更新日期:2000-10-01 00:00:00

  • A simple method for high molecular-weight genomic DNA extraction suitable for long-read sequencing from spores of an obligate biotroph oomycete.

    abstract::Long-read sequencing technologies are having a major impact on our approaches to studying non-model organisms and microbial communities. By significantly reducing the cost and facilitating the genome assembly pipelines, any laboratory can now develop its own genomics program regardless of the complexity of the genome ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.106054

    authors: Penouilh-Suzette C,Fourré S,Besnard G,Godiard L,Pecrix Y

    更新日期:2020-09-12 00:00:00

  • Evaluation of molecular assays for identification Campylobacter fetus species and subspecies and development of a C. fetus specific real-time PCR assay.

    abstract::Phenotypic differentiation between Campylobacter fetus (C. fetus) subspecies fetus and C. fetus subspecies venerealis is hampered by poor reliability and reproducibility of biochemical assays. AFLP (amplified fragment length polymorphism) and MLST (multilocus sequence typing) are the molecular standards for C. fetus s...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2013.06.005

    authors: van der Graaf-van Bloois L,van Bergen MA,van der Wal FJ,de Boer AG,Duim B,Schmidt T,Wagenaar JA

    更新日期:2013-10-01 00:00:00

  • A dynamic regression analysis tool for quantitative assessment of bacterial growth written in Python.

    abstract::Herein, an open-source method to generate quantitative bacterial growth data from high-throughput microplate assays is described. The bacterial lag time, maximum specific growth rate, doubling time and delta OD are reported. Our method was validated by carbohydrate utilization of lactobacilli, and visual inspection re...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2016.11.015

    authors: Hoeflinger JL,Hoeflinger DE,Miller MJ

    更新日期:2017-01-01 00:00:00

  • Comparison of PCR and serotyping of Group B Streptococcus in pregnant women: the Oslo GBS-study.

    abstract::Streptococcus agalactiae (GBS) is a leading cause of invasive neonatal infection. Serotyping of GBS is important in following epidemiological trends and vaccine development. Capsular serotyping of GBS by latex agglutination has been the predominant typing method, but more recently capsular genotyping has been introduc...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2014.11.001

    authors: Brigtsen AK,Dedi L,Melby KK,Holberg-Petersen M,Radtke A,Lyng RV,Andresen LL,Jacobsen AF,Fugelseth D,Whitelaw A

    更新日期:2015-01-01 00:00:00

  • Long term storage of fastidious bacteria (Neisseria spp. and Haemophilus spp.) with swab preservation at -80 °C.

    abstract::Preservation of strains is important to further studies and characterization; we have shown a technique of long-term preservation of Neisseria spp. and Haemophilus spp. by freezing cotton tip swabs impregnated with fastidious organisms at -80 °C without the addition of cryoprotectants. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/j.mimet.2020.105969

    authors: Villa L,Torreblanca A,Otero L,Carreño F,Campo R,López-Escobar M,Vazquez F

    更新日期:2020-08-01 00:00:00

  • Quantitative reverse transcription polymerase chain reaction analysis of Porphyromonas gingivalis gene expression in vivo.

    abstract::An etiological relationship between periodontitis, a significant oral health problem, and the anaerobe Porphyromonas gingivalis may be related to the expression of a variety of putative virulence factors. The objective of the experiments described here was to develop a quantitative reverse transcription polymerase cha...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章

    doi:10.1016/s0167-7012(01)00362-1

    authors: Shelburne CE,Gleason RM,Germaine GR,Wolff LF,Mullally BH,Coulter WA,Lopatin DE

    更新日期:2002-04-01 00:00:00