Abstract：
:Thermophilic strains of Geobacillus, Anoxybacillus and Bacillus that are able to grow at 55 degrees C and above are recognized as commonly occurring contaminants during the production of milk powders. In particular, Anoxybacillus flavithermus strain C and Bacillus licheniformis strain F are often the most prevalent. We describe here the development of a TaqMan-based real-time-PCR assay using a small amplicon of the ribosomal 16S rRNA gene for the selective and quantitative detection of thermophilic bacilli in milk powders. We further present an effective, rapid and inexpensive method for the isolation of total bacterial DNA from milk powder for quantitative PCR analysis within 20 min. With this method, the detection of thermophilic bacilli in milk powder can be accomplished within 1 h. The detection limit for reconstituted and inoculated milk was 8 vegetative cfu ml(-1) and 64 spores ml(-1), respectively.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Rueckert A,Ronimus RS,Morgan HWdoi
10.1016/j.mimet.2004.09.008subject
Has Abstractpub_date
2005-02-01 00:00:00pages
155-67issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(04)00256-8journal_volume
60pub_type
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