Abstract:
:Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by approximately 23%.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
O'Sullivan LA,Webster G,Fry JC,Parkes RJ,Weightman AJdoi
10.1016/j.mimet.2008.08.006subject
Has Abstractpub_date
2008-12-01 00:00:00pages
579-81issue
3eissn
0167-7012issn
1872-8359pii
S0167-7012(08)00307-2journal_volume
75pub_type
杂志文章abstract::A new, simple approach for the isolation and quantitative analysis of the cell wall (CW) proteins from invasively growing Saccharomyces cerevisiae strains is described in this contribution. The proposed method was proved compatible with agar-invasion assays and was demonstrated to be useful as a screening tool for rap...
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abstract::Characterization of clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and representatives of three MRSA clones from other hospitals was performed by arbitrarily primed polymerase chain reaction (AP-PCR) and antibiotic susceptibility patterns. All isolates were mecA-positive and two main antibioty...
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abstract::Rothia dentocariosa is an indigenous microbial flora of dental plaque. To examine the bacterium population in oral cavities, a novel selective medium, designated RDSM, was developed for the isolation of R. dentocariosa. RDSM was prepared by adding 0.5 μg/ml of lincomycin, 10 μg/ml of colistin, and 40% sucrose to Heart...
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journal_title:Journal of microbiological methods
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abstract::A method for rapidly and selectively isolating Salmonellae from buffer solutions and concentrating the bacteria by a factor of approximately 500 was developed. Anti-Salmonellae antibody was covalently linked to 40 microm polyacrylamide beads to prepare a solid phase with affinity for the bacteria. The beads were packe...
journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
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journal_title:Journal of microbiological methods
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abstract::The bclA gene codes for the protein backbone of the exosporium glycoprotein BclA of B. anthracis. BclA has a central collagen-like region formed by polymorphic GXX repeats and conserved amino- and carboxy-termini. It is noted here that the bclA gene is also present in the genome of Bacillus cereus and Bacillus thuring...
journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
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journal_title:Journal of microbiological methods
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abstract::Characterization of airborne bacterial cells requires efficient collection, concentration, and analysis techniques, particularly to overcome the challenge of their dilute nature in outdoor environments. This study aims to establish a rapid and reliable approach for quantification of bacteria in air samples. To do this...
journal_title:Journal of microbiological methods
pub_type: 杂志文章
doi:10.1016/j.mimet.2018.10.012
更新日期:2018-11-01 00:00:00
abstract::Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many crops and are used as inoculants. PGPB colonization is an important trait for success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to distinguishing, by fluorescence in situ...
journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
doi:10.1016/s0167-7012(02)00106-9
更新日期:2002-11-01 00:00:00
abstract::Fourier-transform infrared (FT-IR) spectroscopy is known as a high-resolution method for the rapid identification of pure cultures of microorganisms. Here, we evaluated FT-IR as a method for the quantification of bacterial populations in binary mixed cultures consisting of Pseudomonas putida and Rhodococcus ruber. A c...
journal_title:Journal of microbiological methods
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pub_type: 杂志文章,评审
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
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abstract::In this study, we aimed to identify the molecular carbapenemase types of the Enterobacteriaceae isolates and to evaluate the performance of manually prepared and commercially available combination disc methods and the modified Hodge test. One hundred and forty carbapenemase producing isolates and 45 isolates as contro...
journal_title:Journal of microbiological methods
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doi:10.1016/j.mimet.2016.03.010
更新日期:2016-06-01 00:00:00
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journal_title:Journal of microbiological methods
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abstract::We describe a method based on fluorescence in situ hybridisation (FISH) that allows the identification of individual cells by electron microscopy. We hybridised universal and specific fluorescein-labelled oligonucleotide probes to the ribosomal RNA of prokaryotic microorganisms in heterogeneous cell mixtures. We then ...
journal_title:Journal of microbiological methods
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
abstract::Representational Difference Analysis (RDA) is an established technique used for isolation of specific genetic differences between or within bacterial species. This method was used to investigate the genetic basis of serovar-specificity and the relationship between serovar and virulence in Haemophilus parasuis. An RDA ...
journal_title:Journal of microbiological methods
pub_type: 杂志文章
doi:10.1016/j.mimet.2006.09.011
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
doi:10.1016/j.mimet.2012.04.005
更新日期:2012-07-01 00:00:00
abstract::In order to study functional gene expression in Streptomyces coelicolor, a mini-transposon encoding the apramycin resistance gene aac(3)IV within its inverted repeat (IR) boundaries was constructed based on IS204, which was previously identified in the genome of Nocardia asteroides YP21. The mini-transposon and IS204 ...
journal_title:Journal of microbiological methods
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