Abstract：
:Fourier-transform infrared (FT-IR) spectroscopy is known as a high-resolution method for the rapid identification of pure cultures of microorganisms. Here, we evaluated FT-IR as a method for the quantification of bacterial populations in binary mixed cultures consisting of Pseudomonas putida and Rhodococcus ruber. A calibration procedure based on Principal Component Regression was developed for estimating the ratio of the bacterial species. Data for method calibration were gained from pure cultures and artificially assembled communities of known ratios of the two member populations. Moreover, to account for physiological variability, FT-IR measurements were performed with organisms sampled at different growth phases. Measurements and data analyses were subsequently applied to growing mixed cultures revealing that growth of R. ruber was almost completely suppressed in co-culture with P. putida. Population ratios obtained by fatty acid analysis as an independent reference method were in high agreement with the FT-IR derived ratios.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Schäwe R,Fetzer I,Tönniges A,Härtig C,Geyer W,Harms H,Chatzinotas Adoi
10.1016/j.mimet.2011.05.001subject
Has Abstractpub_date
2011-08-01 00:00:00pages
182-7issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(11)00169-2journal_volume
86pub_type
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