Abstract:
:MALDI-ToF MS can be used to identify microorganisms directly from blood cultures. This study compared two methods of sample preparation. Similar levels of genus- (91% vs 90%) and species-level identifications (79% vs 74%) were obtained with differential centrifugation and SDS methods. The SDS method is faster and requires minimal handling.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Robinson AM,Ussher JEdoi
10.1016/j.mimet.2016.05.026subject
Has Abstractpub_date
2016-08-01 00:00:00pages
74-76eissn
0167-7012issn
1872-8359pii
S0167-7012(16)30123-3journal_volume
127pub_type
杂志文章abstract::Group B streptococcus (GBS; Streptococcus agalactiae) serotype III is one of the most common and virulent serotypes of the species. It can be divided into several subtypes, which vary in their distribution among invasive isolates from different patient groups. In this study, we used 91 well-characterized GBS serotype ...
journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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doi:10.1016/j.mimet.2011.12.010
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abstract::Molecular typing is an important tool in surveillance and outbreak investigations of human Salmonella infections. In this study, three molecular typing methods were used to investigate the discriminatory ability, reproducibility and the genetic relationship between 110 Salmonella enterica subspecies enterica isolates....
journal_title:Journal of microbiological methods
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abstract::A simple, rapid, sensitive microtiter plate method detecting N-acyl homoserine lactone (HSL)-related compounds was established using an Agrobacterium tumefaciens strain harboring a traG::lacZ/traR reporter gene responsive to HSLs. This strain did not produce its own HSL, but the traG::lacZ reporter gene was induced on...
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
doi:10.1016/j.mimet.2004.12.013
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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doi:10.1016/j.mimet.2010.07.013
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章,评审
doi:10.1016/j.mimet.2006.11.007
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abstract::Escherichia coli O157:H7 has frequently been associated with foodborne infections and is considered an adulterant in raw non-intact beef in the U.S. Shiga toxin-producing E. coli (STEC) belonging to serogroups O26, O45, O103, O111, O121, and O145 (known as the "big six" non-O157) were estimated to cause >70% of foodbo...
journal_title:Journal of microbiological methods
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abstract::Leishmaniasis, caused by protozoa belonging to the genus Leishmania, is an important public health problem found in >90 countries and with still limited options for treatment. Development of new anti-leishmanial drugs is an urgent need and the identification of new active compounds is a limiting factor that can be acc...
journal_title:Journal of microbiological methods
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abstract::Rapid and accurate detection of the pathogens that cause gastrointestinal infection is important for appropriate therapy and proper infection control. This study assesses the performance of a new molecular assay for simultaneous detection of 13 different gastrointestinal bacteria in stool specimens. Using the Allplex ...
journal_title:Journal of microbiological methods
pub_type: 杂志文章
doi:10.1016/j.mimet.2017.10.016
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abstract::The purpose of this study was to determine the interactions between arsenic (As) resistant bacteria and As, using surface-enhanced Raman scattering (SERS) and Fourier transform infrared (FTIR) spectroscopy. According to our 16S rDNA results, eight bacteria isolated from the environment can be identified to four genera...
journal_title:Journal of microbiological methods
pub_type: 杂志文章
doi:10.1016/j.mimet.2012.03.018
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