Abstract:
:Streptococcus agalactiae (GBS) is a leading cause of invasive neonatal infection. Serotyping of GBS is important in following epidemiological trends and vaccine development. Capsular serotyping of GBS by latex agglutination has been the predominant typing method, but more recently capsular genotyping has been introduced as an alternative method. The purpose of this study was to compare the relative performance of these methods in a contemporary population of pregnant women. We typed isolates from an unselected population of 426 colonized women at delivery using latex agglutination and a combination of four PCR methods. Antibiotic resistance was tested in 449 isolates. Capsular genotyping gave a result in all except three of 426 isolates. Fifty-nine of 426 isolates could not be typed by latex agglutination. Agreement between serotyping and genotyping was shown in 303 (71.1%) of the isolates. 10.2% of the isolates were resistant to erythromycin, 9.6% to clindamycin, 76.6% to tetracycline and none to penicillin. In conclusion, a substantial proportion of the colonizing strains were non-typeable by serotyping, but typeable by genotyping. This suggests that a diagnostic genotyping strategy is preferable to serotyping of the GBS polysaccharide capsule in colonized, pregnant women.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Brigtsen AK,Dedi L,Melby KK,Holberg-Petersen M,Radtke A,Lyng RV,Andresen LL,Jacobsen AF,Fugelseth D,Whitelaw Adoi
10.1016/j.mimet.2014.11.001subject
Has Abstractpub_date
2015-01-01 00:00:00pages
31-5eissn
0167-7012issn
1872-8359pii
S0167-7012(14)00307-8journal_volume
108pub_type
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