Improved detection of microbial DNA after bead-beating before DNA isolation.

Abstract:

:Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.

journal_name

J Microbiol Methods

authors

de Boer R,Peters R,Gierveld S,Schuurman T,Kooistra-Smid M,Savelkoul P

doi

10.1016/j.mimet.2009.11.009

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

209-11

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(09)00375-3

journal_volume

80

pub_type

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