Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR.

Abstract:

:The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the MOTHUR software.

journal_name

J Microbiol Methods

authors

Yang S,Liebner S,Alawi M,Ebenhöh O,Wagner D

doi

10.1016/j.mimet.2014.05.006

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

3-5

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(14)00127-4

journal_volume

103

pub_type

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