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Identification of mutans streptococcal species by the PCR products of the dex genes.

Abstract：

:A pair of polymerase chain reaction (PCR) primers was designed on the basis of the nucleotide sequence homology of dextranase genes (dex) of Streptococcus mutans, S. sobrinus and S. downei. The primer pair amplified a 530-bp DNA fragment on the dex genes of mutans streptococcal species: S. mutans, S. sobrinus, S. downei, S. rattus and S. cricetus. HaeIII digestion of the 530-bp fragments generated species-specific subfragments, which were easily distinguishable from each other by agarose gel electrophoresis. These results suggest that the PCR-amplification of the dex gene followed by the HaeIII digestion is useful for rapid identification of the five species of mutans streptococci.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Igarashi T,Ichikawa K,Yamamoto A,Goto N

doi

10.1016/s0167-7012(01)00263-9

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

99-105

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(01)00263-9

journal_volume

46

pub_type

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