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Development of a method for heterologous gene expression in Enterobacter amnigenus, a potential host for the biological control of mosquito larvi.

Abstract：

:An integrative plasmid containing a 1.3 kb fragment of chromosomal DNA from Enterobacter amnigenus was constructed. The Omega fragment encoding spectinomycin/streptomycin resistance was cloned into the unique BglII site of the resulting plasmid, and the interrupted fragment was transferred via plasmid pMAK705 by electroporation into E. amnigenus with a selection for spectinomycin resistance. Cointegrants were resolved to generate an E. amnigenus strain that expressed spectinomycin resistance, but grew as rapidly as the parental strain. The cloned fragment encodes a putative homologue of the proW gene of Escherichia coli that is not essential for E. amnigenus growth. The integrative plasmid is now available to introduce any heterologous DNA into the E. amnigenus chromosome, for the construction of promoter-probe vectors for the studies of gene regulation, or to construct plasmids suitable for the isolation of secretion signals. Immediate applications of this system will include the expression and secretion of crystal toxins from bacilli for the biological control of mosquito larvae infected with the bacterial host.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Nantapong N,Tanapongpipat S,Cole J,Panyim S

doi

10.1016/s0167-7012(01)00384-0

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

329-34

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167701201003840

journal_volume

49

pub_type

杂志文章

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