Abstract:
:Chlamydophila pneumoniae (C. pneumoniae) has been detected in macrophages (Mø) and dendritic cells (DC) in vascular diseases. To understand the importance of these cell types in C. pneumoniae infection and transmission, we infected DC and cultivated them with Mø in a coculture model system which precludes cell-to-cell contact during chlamydial infection. C. pneumoniae inside living DC were labeled and tracked with a red fluorescent ceramide dye. Subsequently, red-coloured chlamydial inclusions were detected 3 and 5 days later in cocultured Mø. Moreover, standard assays revealed infectious elementary bodies in infected DC and cocultured Mø. Assays for chlamydial gene expression indicated vital and dividing chlamydiae in both cell types. In summary, the results suggest that the transwell system employed here is a suitable model to investigate the transmission of C. pneumoniae from DC to Mø. Importantly, the observations presented demonstrate that transmission is independent of cell-to-cell contact.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Wittkop U,Peppmueller M,Njau F,Leibold W,Klos A,Krausse-Opatz B,Hudson AP,Zeidler H,Haller H,Wagner ADdoi
10.1016/j.mimet.2007.12.010subject
Has Abstractpub_date
2008-03-01 00:00:00pages
288-95issue
3eissn
0167-7012issn
1872-8359pii
S0167-7012(07)00426-5journal_volume
72pub_type
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