Abstract:
:Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I-positive strain which may be due to solvent inaccessibility of epitope residues on intact fimbriae.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Savar NS,Dashti A,Darzi Eslam E,Jahanian-Najafabadi A,Jafari Adoi
10.1016/j.mimet.2014.07.035subject
Has Abstractpub_date
2014-11-01 00:00:00pages
40-46eissn
0167-7012issn
1872-8359pii
S0167-7012(14)00231-0journal_volume
106pub_type
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