Antigenicity and immunogenicity of fused B-subunit of heat labile toxin of Escherichia coli and colonization factor antigen I polyepitopes.

Abstract:

:Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I-positive strain which may be due to solvent inaccessibility of epitope residues on intact fimbriae.

journal_name

J Microbiol Methods

authors

Savar NS,Dashti A,Darzi Eslam E,Jahanian-Najafabadi A,Jafari A

doi

10.1016/j.mimet.2014.07.035

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

40-46

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(14)00231-0

journal_volume

106

pub_type

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