Abstract:
:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and chromogenic media are widely used in clinical microbiology laboratories to facilitate the rapid selection and identification of pathogens. The aim of this study was to evaluate whether usage of chromogenic media limits the diagnostic performance of MALDI-TOF MS for microbial identification. A total of 386 microorganisms collected and analyzed at five laboratories were included. Isolates were cultured on relevant chromogenic media and non-selective agar plates in parallel and identified using the Bruker MALDI-TOF MS. Among the tested isolates, no misidentification was recorded and there was no medium-related difference in the identification level. However, score values were overall slightly but significantly lower for isolates grown on chromogenic media. In conclusion, the use of chromogenic culture media tested here had no relevant impact on MALDI-TOF MS performance for diagnostic purposes.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Lüthje P,Pranada AB,Carruthers-Lay D,Desjardins M,Gaillot O,Wareham D,Ciesielczuk H,Özenci Vdoi
10.1016/j.mimet.2017.03.001subject
Has Abstractpub_date
2017-05-01 00:00:00pages
17-20eissn
0167-7012issn
1872-8359pii
S0167-7012(17)30062-3journal_volume
136pub_type
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