A development and an improvement of selectable markers in Pleurotus ostreatus transformation.

Abstract:

:Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7±11.5transformants/μg plasmid DNA.

journal_name

J Microbiol Methods

authors

Matsunaga Y,Ando M,Izumitsu K,Suzuki K,Honda Y,Irie T

doi

10.1016/j.mimet.2017.01.007

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

27-29

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(17)30007-6

journal_volume

134

pub_type

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