Abstract：
:Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Wilharm G,Lepka D,Faber F,Hofmann J,Kerrinnes T,Skiebe Edoi
10.1016/j.mimet.2009.12.002subject
Has Abstractpub_date
2010-02-01 00:00:00pages
215-6issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(09)00383-2journal_volume
80pub_type
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