Development of a L-rhamnose and D-arabitol supplemented MacConkey agar to identify pathogenic Yersinia enterocolitica among environmental Yersinias in swine production wastes.


:Supplemented MacConkey agar with L-rhamnose and D-arabitol distinguishes pathogenic from environmental strains of Yersinia enterocolitica recovered from swine production wastes. This medium has a sensitivity of 100% and a specificity of 97.4%.


J Microbiol Methods


Shehee MW,Sobsey MD




Has Abstract


2004-05-01 00:00:00














  • Performance evaluation of BD Phoenix™, an automated microbiology system, for the screening of IMP-producing Enterobacteriaceae.

    abstract::BD Phoenix™ is an automated bacterial identification and susceptibility testing system. Here, its performance in screening IMP-producing Enterobacteriaceae was evaluated. The system identified 97.8% of IMP producers as being nonsusceptible to imipenem or meropenem, which was higher than that identified by the broth mi...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Yamakawa H,Kosai K,Kawamoto Y,Akamatsu N,Matsuda J,Kaku N,Uno N,Morinaga Y,Hasegawa H,Yanagihara K

    更新日期:2018-02-01 00:00:00

  • High density whole genome fingerprinting of methicillin-resistant and -susceptible strains of Staphylococcus aureus in search of phenotype-specific molecular determinants.

    abstract::AFLP is a selective restriction fragment amplification method generating DNA fingerprints for microbial isolates. We present high-throughput AFLP (htAFLP) to characterize molecular markers associated with bacterial phenotypes. Methicillin-resistant and -susceptible isolates of Staphylococcus aureus have been used for ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Savelkoul PH,Melles DC,Buffing N,Gorkink R,Simons G,van Belkum A

    更新日期:2007-10-01 00:00:00

  • Codon-improved fluorescent proteins in investigation of Staphylococcus aureus host pathogen interactions.

    abstract::Here we present the use of three fluorescent proteins in Staphylococcus aureus, Cerulean, PA-GFP, and mRFPmars. All molecules have an improved codon adaptation for expression in the A + T rich organisms and extend the fluorescent protein portfolio in staphylococcal research. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Paprotka K,Giese B,Fraunholz MJ

    更新日期:2010-10-01 00:00:00

  • An improved high throughput sequencing method for studying oomycete communities.

    abstract::Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sapkota R,Nicolaisen M

    更新日期:2015-03-01 00:00:00

  • Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR.

    abstract::The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the M...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Yang S,Liebner S,Alawi M,Ebenhöh O,Wagner D

    更新日期:2014-08-01 00:00:00

  • New avenues of controlling microbial infections through anti-microbial and anti-biofilm potentials of green mono-and multi-metallic nanoparticles: A review.

    abstract::Nanoparticles synthesized through the green route deserve special mention because this green technology is not only energy-efficient and cost-effective but also amenable to the environment. Various biological resources have been used for the generation of these 'green nanoparticles'. Biological wastes have also been f...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Das P,Karankar VS

    更新日期:2019-12-01 00:00:00

  • Automatic Bacillus anthracis bacteria detection and segmentation in microscopic images using UNet+.

    abstract::Anthrax is one of the important diseases in humans and animals, caused by the gram-positive bacteria spores called Bacillus anthracis. The disease is still one of the health problems of developing countries. Due to fatigue and decreased visual acuity, microscopic diagnosis of diseases by humans may not be of good qual...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Hoorali F,Khosravi H,Moradi B

    更新日期:2020-10-01 00:00:00

  • A custom amplicon sequencing approach to detect resistance associated mutations and sequence types in Mycoplasma genitalium.

    abstract:BACKGROUND:Mycoplasma genitalium resistance to antibiotic treatments is increasing, with very limited treatment alternatives on the horizon. Surveillance via sequencing of multiple M. genitalium loci would allow: monitoring of known antibiotic resistance mutations, associations between resistance/treatment failure and ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Plummer EL,Murray GL,Bodiyabadu K,Su J,Garland SM,Bradshaw CS,Read TRH,Tabrizi SN,Danielewski JA

    更新日期:2020-12-01 00:00:00

  • Quantification of bacterial invasion into adherent cells by flow cytometry.

    abstract::Quantification of invasive, intracellular bacteria is critical in many areas of cellular microbiology and immunology. We describe a novel and fast approach to determine invasion of bacterial pathogens in adherent cell types such as epithelial cells or fibroblasts based on flow cytometry. Using the CEACAM-mediated upta...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Pils S,Schmitter T,Neske F,Hauck CR

    更新日期:2006-05-01 00:00:00

  • Evaluation of molecular assays for identification Campylobacter fetus species and subspecies and development of a C. fetus specific real-time PCR assay.

    abstract::Phenotypic differentiation between Campylobacter fetus (C. fetus) subspecies fetus and C. fetus subspecies venerealis is hampered by poor reliability and reproducibility of biochemical assays. AFLP (amplified fragment length polymorphism) and MLST (multilocus sequence typing) are the molecular standards for C. fetus s...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: van der Graaf-van Bloois L,van Bergen MA,van der Wal FJ,de Boer AG,Duim B,Schmidt T,Wagenaar JA

    更新日期:2013-10-01 00:00:00

  • A simple binomial test for estimating sequencing errors in public repository 16S rRNA sequences.

    abstract::Sequences in public databases may contain a number of sequencing errors. A double binomial model describing the distribution of indel-excluded similarity coefficients (S) among repeatedly sequenced 16S rRNA was previously developed and it produced a confidence interval of S useful for testing sequence identity among s...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Zo YG,Colwell RR

    更新日期:2008-02-01 00:00:00

  • A new chromogenic agar medium for detection of potentially virulent Yersinia enterocolitica.

    abstract::Several outbreaks of foodborne yersiniosis have been documented and this disease continues to be source of infections transmitted through foods. The selective agars most commonly used to isolate Yersinia enterocolitica in clinical, food and environmental samples, cefsulodin-irgasan-novobiocin (CIN) and MacConkey (MAC)...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Weagant SD

    更新日期:2008-02-01 00:00:00

  • qPCR assays to quantify genes and gene expression associated with microbial perchlorate reduction.

    abstract::Quantitative PCR (qPCR) assays targeting cld (developed in this work) and pcrA (previously described) were used to quantify these perchlorate-related genes in a perchlorate-reducing enrichment culture. Transcript copies were quantified in perchlorate-reducing Rhodocyclaceae strain JDS4. Oxygen and nitrate inhibited ex...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: De Long SK,Kinney KA,Kirisits MJ

    更新日期:2010-11-01 00:00:00

  • Efficient Mutagenesis Independent of Ligation (EMILI).

    abstract::Site-directed mutagenesis is one of the most widely used techniques in life sciences. Here we describe an improved and simplified method for introducing mutations at desired sites. It consists of an inverse PCR using a plasmid template and two partially complementary primers. The synthesis step is followed by annealin...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Füzik T,Ulbrich P,Ruml T

    更新日期:2014-11-01 00:00:00

  • Proteome mining for the identification and in-silico characterization of putative drug targets of multi-drug resistant Clostridium difficile strain 630.

    abstract::Clostridium difficile is an enteric pathogen that causes approximately 20% to 30% of antibiotic-associated diarrhea. In recent years, there has been a substantial rise in the rate of C. difficile infections as well as the emergence of virulent and antibiotic resistant C. difficile strains. So, there is an urgent need ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Lohani M,Dhasmana A,Haque S,Wahid M,Jawed A,Dar SA,Mandal RK,Areeshi MY,Khan S

    更新日期:2017-05-01 00:00:00

  • Random tag insertions by Transposon Integration mediated Mutagenesis (TIM).

    abstract::Transposon Integration mediated Mutagenesis (TIM) is a broadly applicable tool for protein engineering. This method combines random integration of modified bacteriophage Mu transposons with their subsequent defined excision employing type IIS restriction endonuclease AarI. TIM enables deletion or insertion of an arbit...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Hoeller BM,Reiter B,Abad S,Graze I,Glieder A

    更新日期:2008-10-01 00:00:00

  • Comparative performance of Aspergillus galactomannan ELISA and PCR in sputum from patients with ABPA and CPA.

    abstract:OBJECTIVES:Galactomannan (GM) and Aspergillus DNA detection are useful tools for the diagnosis of invasive pulmonary aspergillosis (IPA), primarily in blood and bronchoscopy samples. This study aimed to evaluate the utility of both markers for detection of Aspergillus in sputum from patients with allergic bronchopulmon...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Fayemiwo S,Moore CB,Foden P,Denning DW,Richardson MD

    更新日期:2017-09-01 00:00:00

  • Use of the frc gene as a molecular marker to characterize oxalate-oxidizing bacterial abundance and diversity structure in soil.

    abstract::Oxalate catabolism, which can have both medical and environmental implications, is performed by phylogenetically diverse bacteria. The formyl-CoA-transferase gene was chosen as a molecular marker of the oxalotrophic function. Degenerated primers were deduced from an alignment of frc gene sequences available in databas...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Khammar N,Martin G,Ferro K,Job D,Aragno M,Verrecchia E

    更新日期:2009-02-01 00:00:00

  • Improving efficiency of viability-PCR for selective detection of live cells.

    abstract::Viability PCR (v-PCR) as a method to selectively detect intact live cells has gained considerable interest over the last years with an increasing number of applications. The principle is based on treatment of microbiological samples with a viability dye prior to extraction of genomic DNA and its amplification. The dye...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Nkuipou-Kenfack E,Engel H,Fakih S,Nocker A

    更新日期:2013-04-01 00:00:00

  • Strain development and optimized fermentation conditions for blood meal using Aspergillus niger and Aspergillus oryzae.

    abstract::To hydrolyze blood meal (BM) effectively, two mutants were generated using ultra-violet mutagenesis. Single-factor experiments, the Plackett-Burman technique and response surface methodology were used to optimize the fermentation conditions. This study successfully generated a mutant and innovatively provided importan...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Zheng Y,Zhang H,Wang D,Gao P,Shan A

    更新日期:2014-06-01 00:00:00

  • Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus.

    abstract::The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were obtained showing re...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Schubert M,Stührk C,Fuhr MJ,Schwarze FW

    更新日期:2013-11-01 00:00:00

  • Use of both 16S rRNA and engineered functional genes with real-time PCR to quantify an engineered, PCB-degrading Rhodococcus in soil.

    abstract::A real-time PCR (RTm-PCR) assay using fluorescently labeled oligonucleotides (TaqMan probes) was used to detect and quantify the recombinant Rhodococcus sp. strain RHA1(fcb) in soil. One primer and probe set targeted a hypervariable region of the 16S rRNA gene unique to strain RHA1(fcb) and its phylogenetic relatives,...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Rodrigues JL,Aiello MR,Urbance JW,Tsoi TV,Tiedje JM

    更新日期:2002-10-01 00:00:00

  • The effect of electromagnetic field on antimicrobial activity of lime oil.

    abstract::The antimicrobial activity of lime oil was significantly enhanced against Staphylococcus epidermidis in a pulsed electromagnetic field. These findings support other workers who found that electromagnetic fields augment antimicrobial activity. ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Matewele P

    更新日期:2010-11-01 00:00:00

  • An automated technique for most-probable-number (MPN) analysis of densities of phagotrophic protists with lux AB labelled bacteria as growth medium.

    abstract::An automated modification of the most-probable-number (MPN) technique has been developed for enumeration of phagotrophic protozoa. The method is based on detection of prey depletion in micro titre plates rather than on presence of protozoa. A transconjugant Pseudomonas fluorescens DR54 labelled with a luxAB gene casse...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Ekelund F,Christensen S,Rønn R,Buhl E,Jacobsen CS

    更新日期:1999-11-01 00:00:00

  • Investigations of structure and metabolism within Shewanella oneidensis MR-1 biofilms.

    abstract::Biofilms possess spatially and temporally varying metabolite concentration profiles at the macroscopic and microscopic scales. This results in varying growth environments that may ultimately drive species diversity, determine biofilm structure and the spatial distribution of the community members. Using non-invasive n...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: McLean JS,Majors PD,Reardon CL,Bilskis CL,Reed SB,Romine MF,Fredrickson JK

    更新日期:2008-07-01 00:00:00

  • Multiple-locus variable-number of tandem-repeats analysis distinguishes Vibrio parahaemolyticus pandemic O3:K6 strains.

    abstract::A specific serotype of Vibrio parahaemolyticus, O3:K6, has recently been linked to epidemics of gastroenteritis in Southeast Asia, Japan, and North America. These pandemic O3:K6 strains appear to have recently spread across continents from a single origin to reach global coverage, based on profiling of strains by seve...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Kimura B,Sekine Y,Takahashi H,Tanaka Y,Obata H,Kai A,Morozumi S,Fujii T

    更新日期:2008-03-01 00:00:00

  • Candida albicans viability after exposure to amphotericin B: assessment using metabolic assays and colony forming units.

    abstract::Metabolic assays are a preferred method for evaluation of Candida albicans viability after exposure to antimicrobial agents in cases in which the culture is a complex mixture of yeast and filamentous forms. There is a lack of published data indicating the strength of the correlation between metabolic assays and viable...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Khot PD,Suci PA,Tyler BJ

    更新日期:2008-03-01 00:00:00

  • Comparison of microbiological culture methods in screening allograft tissue. Swab versus nutrient broth.

    abstract::In order to reduce the risk of transmission of infectious diseases through transplantation of tissue allografts, one should examine tissues for the presence of microorganisms. However, there are no detailed tissue banking guidelines describing the culture method or incubation time to be used. Therefore, we compared tw...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Saegeman VS,Lismont D,Verduyckt B,Ectors NL,Verhaegen J

    更新日期:2007-08-01 00:00:00

  • Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-13C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface.

    abstract::Specific fatty acids from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) recovered from a per 13C-labeled bacteria can be detected in environmental samples and used as measures of bacterial transport in the subsurface. Detection of palmitic acid (16:0) and oleic acid (18:1) at m/z 271 (255+16) and 299 (28...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Lytle CA,Fuller ME,Gan YM,Peacock A,DeFlaun MF,Onstott TC,White DC

    更新日期:2001-04-01 00:00:00

  • Development and validation of evolutionary algorithm software as an optimization tool for biological and environmental applications.

    abstract::A flexible, extendable tool for the optimization of (micro)biological processes and protocols using evolutionary algorithms was developed. It has been tested using three different theoretical optimization problems: 2 two-dimensional problems, one with three maxima and one with five maxima and a river autopurification ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sys K,Boon N,Verstraete W

    更新日期:2004-06-01 00:00:00