当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > Rapid antibiotic susceptibility testing by resazurin using thin film platinum as a bio-electrode.

Rapid antibiotic susceptibility testing by resazurin using thin film platinum as a bio-electrode.

Abstract：

:Traditional antibiotic susceptibility testing methods take several days to confirm and start disease treatment. The lack of new antibiotics or drugs warrants a need for optimization of current diagnostic tools for immediate antibiotic susceptibility testing. We present a rapid screening method to evaluate the response of bacteria to antibiotics based upon the electrochemical measurement of live bacterial cell metabolic activity using an electroactive redox dye, resazurin. A thin film of Pt deposited over a glass substrate using the direct current sputtering technique was used as a working bio-electrode for electrochemical readouts. X-ray diffraction and scanning electron microscopy was carried out to characterize the Pt thin film. We tested the efficacy of the method using two different strains, Klebsiella pneumoniae (ATCC-700603) and Escherichia coli (ATCC-25922), against ampicillin, kanamycin and tetracycline. The dye, on incubation with viable bacteria, undergoes reduction and lowers the corresponding peak current value. However, in the presence of an effective antibacterial agent, reduction did not occur due to bacterial cell death and absence of a reducing environment. The electrochemical changes in peak current values were monitored using the differential pulse voltammetry technique and interpretation of the results obtained was based upon changes in peak current values. Our results depict a new methodology where a concentration of 104 cells/mL cells can be detected in less than 4 h. The results were also compared with the conventional disc diffusion method for susceptibility testing which has a bacterial incubation time of 18-24 h. The method can potentially be used for monitoring the susceptibility of bacterial strains towards existing antibacterial agents in an easy, rapid, reliable and inexpensive manner without any pre-cultivation of bacteria.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Mishra P,Singh D,Mishra KP,Kaur G,Dhull N,Tomar M,Gupta V,Kumar B,Ganju L

doi

10.1016/j.mimet.2019.05.009

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

69-76

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(19)30180-0

journal_volume

162

pub_type

杂志文章

相关文献

JOURNAL OF MICROBIOLOGICAL METHODS文献大全
  • Orientation and expression of methicillin-resistant Staphylococcus aureus small RNAs by direct multiplexed measurements using the nCounter of NanoString technology.

    abstract：:Staphylococcus aureus is a versatile bacterial opportunist responsible for a wide spectrum of infections. Several genomes of this major human pathogen have been publicly available for almost 10 years, but comprehensive links between virulence or epidemicity and genome content of the bacterium are still missing. This p...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.12.025

    authors： Beaume M,Hernandez D,Docquier M,Delucinge-Vivier C,Descombes P,François P

    更新日期：2011-02-01 00:00:00

  • A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material.

    abstract：:This study investigated the various commercially available kits and 'in-house' methods to extract DNA from Gram-negative and Gram-positive bacteria, yeast and fungal agents in commonly employed blood culture material. The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template prepar...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(00)00174-3

    authors： Millar BC,Jiru X,Moore JE,Earle JA

    更新日期：2000-10-01 00:00:00

  • Adhesion of Yersinia enterocolitica to non-cultured epithelial cells from pig and rabbit ilea.

    abstract：:A simple and reliable method was developed to isolate intact epithelial cells from pig and rabbit ilea and these were used to investigate the adhesion of Yersinia enterocolitica. Hydrophobic interaction was eliminated by treating the bacterial culture with 0.8 M tetramethyl urea (TMU). Virulent strains of Y. enterocol...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.08.009

    authors： Hussein HM,Fenwick SG,Lumsden JS

    更新日期：2007-02-01 00:00:00

  • Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers.

    abstract：:A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.09.002

    authors： Santos CS,Ramos JN,Vieira VV,Pinheiro CS,Meyer R,Alcantara-Neves NM,Ramos RT,Silva A,Hirata R Jr,Felicori L,de Alegría Puig CR,Navas J,Azevedo V,Mattos-Guaraldi AL,Pacheco LGC

    更新日期：2017-11-01 00:00:00

  • Laser interferometric and cultivation methods for measurement of colistin/ampicilin and saponin interactions with smooth and rough of Proteus mirabilis lipopolysaccharides and cells.

    abstract：:Laser interferometry is commonly used in permeability studies of soluble substances. In this study a modification that allowed testing partially insoluble mixtures is presented. The modification relies on the measurement of diffusion from 1% agarose gel. As a model for this study, two Proteus mirabilis strains were us...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.01.020

    authors： Arabski M,Wasik S,Dworecki K,Kaca W

    更新日期：2009-05-01 00:00:00

  • Assessing the performance of novel software Strain Solution on automated discrimination of Escherichia coli serotypes and their mixtures using matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    abstract：:O157, O26, and O111 are the most important O serogroups of enterohemorrhagic Escherichia coli worldwide. Recently we reported a strategy for discriminating these serotypes from the others using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) based on the S10-spc-alpha operon...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.11.005

    authors： Ojima-Kato T,Yamamoto N,Iijima Y,Tamura H

    更新日期：2015-12-01 00:00:00

  • Pictures of microbiology -- the biofilm imaging facility under Dr. David C. White.

    abstract：:This contribution honoring David C. White (DC) summarizes the five years I interacted with him on a daily basis in his laboratory. Over this time we worked on many different projects all tied together by the unifying principle now recognized as central to bacterial life in nature: biofilms. My goal is to convey some o...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.07.003

    authors： Palmer RJ Jr

    更新日期：2008-07-01 00:00:00

  • A quantitative analysis of DNA extraction and purification from compost.

    abstract：:We quantified both DNA and humic acid concentrations during the extraction and purification of DNA from compost. The DNA extraction method consisted of bead-beating with SDS for cell lysis, poly(ethylene glycol)-8000 precipitation for preliminary DNA purification, and chromatography on a 10-ml Sephadex G-200 column fo...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(03)00006-x

    authors： Howeler M,Ghiorse WC,Walker LP

    更新日期：2003-07-01 00:00:00

  • An evaluation of the impact of clinical bacterial isolates on epithelial cell monolayer integrity by the electric Cell-Substrate Impedance Sensing (ECIS) method.

    abstract：:Virulence is the relative capacity of a pathogenic microorganism to cause damage in susceptible host cells such as those found in airway passages and the gut. In this study, the effect of clinical bacterial isolates on the monolayer integrity of cultured human alveolar basal epithelial cells (A549) was evaluated using...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2020.105833

    authors： Nahid MA,Campbell CE,Fong KSK,Barnhill JC,Washington MA

    更新日期：2020-02-01 00:00:00

  • Detection of extended-spectrum β-lactamases producing Enterobacteriaceae using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based MBT STAR-BL software module with β-lactamase inhibition assay depends on the bacterial stra

    abstract：:Rapid and sensitive detection of extended-spectrum β-lactamases (ESBLs) is essential for infection control and antimicrobial treatment. Recently, a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based MBT STAR-BL software module has been used for detecting β-lactamase activ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.105734

    authors： Ota Y,Furuhashi K,Nagao Y,Nanba T,Yamanaka K,Ishikawa J,Nagura O,Iwaizumi M,Hamada E,Maekawa M

    更新日期：2019-12-01 00:00:00

  • Interrogation of single nucleotide polymorphisms in gnd provides a novel method for molecular serogrouping of clinically important Shiga toxin producing Escherichia coli (STEC) targeted by regulation in the United States, including the "big six" non-O157

    abstract：:Escherichia coli O157:H7 has frequently been associated with foodborne infections and is considered an adulterant in raw non-intact beef in the U.S. Shiga toxin-producing E. coli (STEC) belonging to serogroups O26, O45, O103, O111, O121, and O145 (known as the "big six" non-O157) were estimated to cause >70% of foodbo...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.07.005

    authors： Elder JR,Bugarel M,den Bakker HC,Loneragan GH,Nightingale KK

    更新日期：2016-10-01 00:00:00

  • Optimization of a Nile Red method for rapid lipid determination in autotrophic, marine microalgae is species dependent.

    abstract：:Several studies have been conducted to develop rapid methods for quantification of lipid content in microalgae, as an alternative for time consuming gravimetric methods. Different studies showed that lipid staining with Nile Red in whole cell suspensions and subsequently quantification by the use of a spectrofluoromet...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.09.009

    authors： Balduyck L,Veryser C,Goiris K,Bruneel C,Muylaert K,Foubert I

    更新日期：2015-11-01 00:00:00

  • Rapid differentiation of bacterial communities using high resolution melting analysis.

    abstract：:Analysis of microbial communities is of broad interest in biology and high throughput sequencing is now the preferred method. However, some studies may not need the level of detail high throughput sequencing provides and its cost may limit the number of samples that can be sequenced. High resolution melting analysis (...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.07.006

    authors： Everman S,Wang SY

    更新日期：2017-09-01 00:00:00

  • Addition of thymidine to culture media for accurate examination of thymidine-dependent small-colony variants of methicillin-resistant Staphylococcus aureus: a pilot study.

    abstract：:Small-colony variants (SCVs) are slow-growing subpopulations of various auxotrophic bacterial strains. Thymidine-dependent SCVs (TD-SCVs) are unable to synthesize thymidine; hence, these variants fail to grow in a medium without thymidine. In this study, we used 10 TD-SCVs of Staphylococcus aureus, of which four strai...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.01.007

    authors： Horiuchi K,Matsumoto T,Ota Y,Kasuga E,Negishi T,Yaguchi T,Sugano M,Honda T

    更新日期：2015-03-01 00:00:00

  • Improving qPCR efficiency in environmental samples by selective removal of humic acids with DAX-8.

    abstract：:Quantitative PCR is becoming the method of choice for the detection of pathogenic microorganisms and other targets in the environment. A major obstacle when amplifying DNA is the presence of inhibiting substances like humic acids that decrease the efficiency of PCR. We combined the polymeric adsorbent Supelite™ DAX-8 ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2010.12.027

    authors： Schriewer A,Wehlmann A,Wuertz S

    更新日期：2011-04-01 00:00:00

  • Efficient Mutagenesis Independent of Ligation (EMILI).

    abstract：:Site-directed mutagenesis is one of the most widely used techniques in life sciences. Here we describe an improved and simplified method for introducing mutations at desired sites. It consists of an inverse PCR using a plasmid template and two partially complementary primers. The synthesis step is followed by annealin...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.08.003

    authors： Füzik T,Ulbrich P,Ruml T

    更新日期：2014-11-01 00:00:00

  • Robust biofilm assay for quantification and high throughput screening applications.

    abstract：:Bacterial biofilms are populations of bacteria within a self-produced adherent extracellular matrix that are notoriously resistant to treatment. Existing methods for biofilm quantification are often limited in their dynamic range of detection (signal-to-background), throughput, and require modifications to the protoco...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.02.018

    authors： Rajamani S,Sandy R,Kota K,Lundh L,Gomba G,Recabo K,Duplantier A,Panchal RG

    更新日期：2019-04-01 00:00:00

  • Comparison of multiplex PCR hybridization-based and singleplex real-time PCR-based assays for detection of low prevalence pathogens in spiked samples.

    abstract：:Molecular diagnostic devices are increasingly finding utility in clinical laboratories. Demonstration of the effectiveness of these devices is dependent upon comparing results from clinical samples tested with the new device to an alternative testing method. The preparation of mock clinical specimens will be necessary...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.11.005

    authors： Hockman D,Dong M,Zheng H,Kumar S,Huff MD,Grigorenko E,Beanan M,Duncan R

    更新日期：2017-01-01 00:00:00

  • A bench-top automated workstation for nucleic acid isolation from clinical sample types.

    abstract：:Systems that automate extraction of nucleic acid from cells or viruses in complex clinical matrices have tremendous value even in the absence of an integrated downstream detector. We describe our bench-top automated workstation that integrates our previously-reported extraction method - TruTip - with our newly-develop...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2018.03.021

    authors： Thakore N,Garber S,Bueno A,Qu P,Norville R,Villanueva M,Chandler DP,Holmberg R,Cooney CG

    更新日期：2018-05-01 00:00:00

  • Use of Enterococcus faecalis and Bacillus atrophaeus as surrogates to establish and maintain laboratory proficiency for concentration of water samples using ultrafiltration.

    abstract：:The U.S. Environmental Protection Agency's (EPA) Water Laboratory Alliance (WLA) currently uses ultrafiltration (UF) for concentration of biosafety level 3 (BSL-3) agents from large volumes (up to 100-L) of drinking water prior to analysis. Most UF procedures require comprehensive training and practice to achieve and ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.08.013

    authors： Mapp L,Klonicki P,Takundwa P,Hill VR,Schneeberger C,Knee J,Raynor M,Hwang N,Chambers Y,Miller K,Pope M

    更新日期：2015-11-01 00:00:00

  • A simple method for high molecular-weight genomic DNA extraction suitable for long-read sequencing from spores of an obligate biotroph oomycete.

    abstract：:Long-read sequencing technologies are having a major impact on our approaches to studying non-model organisms and microbial communities. By significantly reducing the cost and facilitating the genome assembly pipelines, any laboratory can now develop its own genomics program regardless of the complexity of the genome ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2020.106054

    authors： Penouilh-Suzette C,Fourré S,Besnard G,Godiard L,Pecrix Y

    更新日期：2020-09-12 00:00:00

  • Long-term monitoring of biofilm growth and disinfection using a quartz crystal microbalance and reflectance measurements.

    abstract：:A biofilm reactor was constructed to monitor the long-term growth and removal of biofilms as monitored by the use of a quartz crystal microbalance (QCM) and a novel optical method. The optical method measures the reflectance of white light off the surface of the quartz crystal microbalance electrode (gold) for determi...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.01.016

    authors： Reipa V,Almeida J,Cole KD

    更新日期：2006-09-01 00:00:00

  • Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes.

    abstract：:Two rapid in situ enumeration methods using fluorescent probes were used to assess the physiological activities of Klebsiella pneumoniae biofilms on stainless steel. Fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and rhodamine 123 (Rh 123), were chosen to perform this study. CTC is a soluble redox ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/0167-7012(94)90058-2

    authors： Yu FP,McFeters GA

    更新日期：1994-01-01 00:00:00

  • Bacterial identification using a SCIEX 5800 TOF/TOF MALDI research instrument and an external database.

    abstract：:In our current study we were identifying 26 bacterial isolates using a SCIEX 5800 TOF/TOF MALDI instrument and an external database. The results were compared with the results of a Vitek® MS system and in case of discrepancies at the species level 16s rRNA sequencing was performed for further verification. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2019.105685

    authors： Huber CA,Pflüger V,Reed S,Cottrell K,Sidjabat HE,Ranasinghe A,Zowawi HM,Harris P,Paterson DL

    更新日期：2019-09-01 00:00:00

  • Use of the frc gene as a molecular marker to characterize oxalate-oxidizing bacterial abundance and diversity structure in soil.

    abstract：:Oxalate catabolism, which can have both medical and environmental implications, is performed by phylogenetically diverse bacteria. The formyl-CoA-transferase gene was chosen as a molecular marker of the oxalotrophic function. Degenerated primers were deduced from an alignment of frc gene sequences available in databas...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2008.09.020

    authors： Khammar N,Martin G,Ferro K,Job D,Aragno M,Verrecchia E

    更新日期：2009-02-01 00:00:00

  • Application of Amido black staining to enumerating bacteria grown on membrane filters.

    abstract：:A method to detect and enumerate bacterial colonies grown on membrane filters (MF) was described. The colonies were stained with an ethanolic solution of 0.1% Amido black 10B. The procedure yielded the rapid detection of colonies as compared to a conventional plate counting method. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(03)00170-2

    authors： Inatomi K

    更新日期：2003-11-01 00:00:00

  • Attenuated colicin-based screening to discover and create novel resistance genes.

    abstract：:I formulated a systematic approach to screen for colicin resistance-associated genes by using an attenuated colicin mutant and demonstrated its utility in a screen of genes related to colicin E5 resistance. Screening of an Escherichia coli genome library revealed rstA as a partial resistance gene to colicin E5. Transc...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.03.003

    authors： Futai K

    更新日期：2014-05-01 00:00:00

  • Universal external RNA controls for microbial gene expression analysis using microarray and qRT-PCR.

    abstract：:Gene expression analysis provides significant insight to understand regulatory mechanisms of biology, yet acquisition and reproduction of quality data, as well as data confirmation and verification remain challenging due to a lack of proper quality controls across different assay platforms. We present a set of six uni...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.10.014

    authors： Liu ZL,Slininger PJ

    更新日期：2007-03-01 00:00:00

  • Robust at-line quantification of poly(3-hydroxyalkanoate) biosynthesis by flow cytometry using a BODIPY 493/503-SYTO 62 double-staining.

    abstract：:Poly(3-hydroxyalkanoates) (PHAs) are bio-based and biodegradable polyesters which have been considered as a promising alternative to petrol-based plastics. Their bacterial production is a dynamic process in which intracellular polymerization and depolymerization are closely linked and depend on the availability of car...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.10.003

    authors： Karmann S,Follonier S,Bassas-Galia M,Panke S,Zinn M

    更新日期：2016-12-01 00:00:00

  • Random tag insertions by Transposon Integration mediated Mutagenesis (TIM).

    abstract：:Transposon Integration mediated Mutagenesis (TIM) is a broadly applicable tool for protein engineering. This method combines random integration of modified bacteriophage Mu transposons with their subsequent defined excision employing type IIS restriction endonuclease AarI. TIM enables deletion or insertion of an arbit...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2008.06.017

    authors： Hoeller BM,Reiter B,Abad S,Graze I,Glieder A

    更新日期：2008-10-01 00:00:00