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The use of the cMyc epitope tag can be problematic for protein detection in Legionella pneumophila.

Abstract：

:In various recent experiments with respect to protein secretion by L. pneumophila, protein detection based on the cMyc-tag seemed to be problematic. For one specific protein, we studied the reason for this problem in more detail by comparing protein expression in the presence and absence of the cMyc-tag. We demonstrated a decrease in the amount of mRNA transcript when the cMyc coding sequence is present. We can conclude that this epitope tag is not a preferential tag for protein detection in L. pneumophila.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

De Buck E,Lebeau I,Van Mellaert L,Geukens N,Anné J,Lammertyn E

doi

10.1016/j.mimet.2004.05.010

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

131-4

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167701204001502

journal_volume

59

pub_type

杂志文章

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